Identification of protein-protein and ribonucleoprotein complexes containing Hfq

Abstract Hfq is a RNA-binding protein that plays a pivotal role in the control of gene expression in bacteria by stabilizing sRNAs and facilitating their pairing with multiple target mRNAs. It has already been shown that Hfq, directly or indirectly, interacts with many proteins: RNase E, Rho, poly(A...

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Autores principales:	Joël Caillet, Bruno Baron, Irina V. Boni, Célia Caillet-Saguy, Eliane Hajnsdorf
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9b8eca38eb3a496aa437f2331b7e5aeb
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spelling	oai:doaj.org-article:9b8eca38eb3a496aa437f2331b7e5aeb2021-12-02T15:09:00ZIdentification of protein-protein and ribonucleoprotein complexes containing Hfq10.1038/s41598-019-50562-w2045-2322https://doaj.org/article/9b8eca38eb3a496aa437f2331b7e5aeb2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-50562-whttps://doaj.org/toc/2045-2322Abstract Hfq is a RNA-binding protein that plays a pivotal role in the control of gene expression in bacteria by stabilizing sRNAs and facilitating their pairing with multiple target mRNAs. It has already been shown that Hfq, directly or indirectly, interacts with many proteins: RNase E, Rho, poly(A)polymerase, RNA polymerase… In order to detect more Hfq-related protein-protein interactions we have used two approaches, TAP-tag combined with RNase A treatment to access the role of RNA in these complexes, and protein-protein crosslinking, which freezes protein-protein complexes formed in vivo. In addition, we have performed microscale thermophoresis to evaluate the role of RNA in some of the complexes detected and used far-western blotting to confirm some protein-protein interactions. Taken together, the results show unambiguously a direct interaction between Hfq and EF-Tu. However a very large number of the interactions of proteins with Hfq in E. coli involve RNAs. These RNAs together with the interacting protein, may play an active role in the formation of Hfq-containing complexes with previously unforeseen implications for the riboregulatory functions of Hfq.Joël CailletBruno BaronIrina V. BoniCélia Caillet-SaguyEliane HajnsdorfNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joël Caillet Bruno Baron Irina V. Boni Célia Caillet-Saguy Eliane Hajnsdorf Identification of protein-protein and ribonucleoprotein complexes containing Hfq
description	Abstract Hfq is a RNA-binding protein that plays a pivotal role in the control of gene expression in bacteria by stabilizing sRNAs and facilitating their pairing with multiple target mRNAs. It has already been shown that Hfq, directly or indirectly, interacts with many proteins: RNase E, Rho, poly(A)polymerase, RNA polymerase… In order to detect more Hfq-related protein-protein interactions we have used two approaches, TAP-tag combined with RNase A treatment to access the role of RNA in these complexes, and protein-protein crosslinking, which freezes protein-protein complexes formed in vivo. In addition, we have performed microscale thermophoresis to evaluate the role of RNA in some of the complexes detected and used far-western blotting to confirm some protein-protein interactions. Taken together, the results show unambiguously a direct interaction between Hfq and EF-Tu. However a very large number of the interactions of proteins with Hfq in E. coli involve RNAs. These RNAs together with the interacting protein, may play an active role in the formation of Hfq-containing complexes with previously unforeseen implications for the riboregulatory functions of Hfq.
format	article
author	Joël Caillet Bruno Baron Irina V. Boni Célia Caillet-Saguy Eliane Hajnsdorf
author_facet	Joël Caillet Bruno Baron Irina V. Boni Célia Caillet-Saguy Eliane Hajnsdorf
author_sort	Joël Caillet
title	Identification of protein-protein and ribonucleoprotein complexes containing Hfq
title_short	Identification of protein-protein and ribonucleoprotein complexes containing Hfq
title_full	Identification of protein-protein and ribonucleoprotein complexes containing Hfq
title_fullStr	Identification of protein-protein and ribonucleoprotein complexes containing Hfq
title_full_unstemmed	Identification of protein-protein and ribonucleoprotein complexes containing Hfq
title_sort	identification of protein-protein and ribonucleoprotein complexes containing hfq
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/9b8eca38eb3a496aa437f2331b7e5aeb
work_keys_str_mv	AT joelcaillet identificationofproteinproteinandribonucleoproteincomplexescontaininghfq AT brunobaron identificationofproteinproteinandribonucleoproteincomplexescontaininghfq AT irinavboni identificationofproteinproteinandribonucleoproteincomplexescontaininghfq AT celiacailletsaguy identificationofproteinproteinandribonucleoproteincomplexescontaininghfq AT elianehajnsdorf identificationofproteinproteinandribonucleoproteincomplexescontaininghfq
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Identification of protein-protein and ribonucleoprotein complexes containing Hfq

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