A multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments

Abstract Tumor tissue contains a continuous distribution of static and dynamically changing oxygen environments with levels ranging from physiologically normal oxygen down to anoxia. However, in vitro studies are often performed under oxygen levels that are far higher than those found in vivo. A num...

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Autores principales:	Ming Yao, Glenn Walker, Michael P. Gamcsik
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9b8ffc8ada3e4cc39c4da97b11ed98ed
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spelling	oai:doaj.org-article:9b8ffc8ada3e4cc39c4da97b11ed98ed2021-12-02T10:54:23ZA multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments10.1038/s41598-021-83579-12045-2322https://doaj.org/article/9b8ffc8ada3e4cc39c4da97b11ed98ed2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83579-1https://doaj.org/toc/2045-2322Abstract Tumor tissue contains a continuous distribution of static and dynamically changing oxygen environments with levels ranging from physiologically normal oxygen down to anoxia. However, in vitro studies are often performed under oxygen levels that are far higher than those found in vivo. A number of devices are available to alter the oxygen environment in cell culture, including designs from our laboratory. However, in our devices and most other designs, changing the media in order to feed or dose cells remains a disruptive factor in maintaining a consistent hypoxic environment. This report presents a novel 96-well plate design that recirculates the local oxygen environment to shield cells during media changes and facilitates toxicity studies of cells cultured under varying oxygen levels. The principle behind the design is presented and the response of human pancreatic cancer PANC-1 cells treated with tirapazamine and doxorubicin under eight different static or cycling oxygen levels was measured. As expected, tirapazamine is progressively more toxic as oxygen levels decrease but retains some toxicity as oxygen is cycled between hypoxic and normoxic levels. Doxorubicin sensitivity is largely unaffected by changing oxygen levels. This technology is ideal for assessing the effects of oxygen as a variable in toxicity screens.Ming YaoGlenn WalkerMichael P. GamcsikNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ming Yao Glenn Walker Michael P. Gamcsik A multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments
description	Abstract Tumor tissue contains a continuous distribution of static and dynamically changing oxygen environments with levels ranging from physiologically normal oxygen down to anoxia. However, in vitro studies are often performed under oxygen levels that are far higher than those found in vivo. A number of devices are available to alter the oxygen environment in cell culture, including designs from our laboratory. However, in our devices and most other designs, changing the media in order to feed or dose cells remains a disruptive factor in maintaining a consistent hypoxic environment. This report presents a novel 96-well plate design that recirculates the local oxygen environment to shield cells during media changes and facilitates toxicity studies of cells cultured under varying oxygen levels. The principle behind the design is presented and the response of human pancreatic cancer PANC-1 cells treated with tirapazamine and doxorubicin under eight different static or cycling oxygen levels was measured. As expected, tirapazamine is progressively more toxic as oxygen levels decrease but retains some toxicity as oxygen is cycled between hypoxic and normoxic levels. Doxorubicin sensitivity is largely unaffected by changing oxygen levels. This technology is ideal for assessing the effects of oxygen as a variable in toxicity screens.
format	article
author	Ming Yao Glenn Walker Michael P. Gamcsik
author_facet	Ming Yao Glenn Walker Michael P. Gamcsik
author_sort	Ming Yao
title	A multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments
title_short	A multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments
title_full	A multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments
title_fullStr	A multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments
title_full_unstemmed	A multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments
title_sort	multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9b8ffc8ada3e4cc39c4da97b11ed98ed
work_keys_str_mv	AT mingyao amultiwellplatebasedsystemfortoxicityscreeningundermultiplestaticorcyclingoxygenenvironments AT glennwalker amultiwellplatebasedsystemfortoxicityscreeningundermultiplestaticorcyclingoxygenenvironments AT michaelpgamcsik amultiwellplatebasedsystemfortoxicityscreeningundermultiplestaticorcyclingoxygenenvironments AT mingyao multiwellplatebasedsystemfortoxicityscreeningundermultiplestaticorcyclingoxygenenvironments AT glennwalker multiwellplatebasedsystemfortoxicityscreeningundermultiplestaticorcyclingoxygenenvironments AT michaelpgamcsik multiwellplatebasedsystemfortoxicityscreeningundermultiplestaticorcyclingoxygenenvironments
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A multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments

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