Phenolic Compound Composition and Biological Activities of Fractionated Soybean Pod Extract

The objective of this study was to determine the potential of dry extracts from soybean pods as a source of bioactive compounds. The phenolic compound composition of the extract (E) and fraction (F1-F3) as well as their respective lipophilicity were analyzed by UPLC-ESI-QTOF-MS. The biological activ...

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Autores principales:	Marzena Pabich, Beata Marciniak, Renata Kontek
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	soybean pods antioxidant activity cytotoxic activity phenolic composition UPLC-ESI-QTOF-MS phenolic compounds Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/9b9547dc559a45e485648dfa2bc56253
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spelling	oai:doaj.org-article:9b9547dc559a45e485648dfa2bc562532021-11-11T15:16:45ZPhenolic Compound Composition and Biological Activities of Fractionated Soybean Pod Extract10.3390/app1121102332076-3417https://doaj.org/article/9b9547dc559a45e485648dfa2bc562532021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/10233https://doaj.org/toc/2076-3417The objective of this study was to determine the potential of dry extracts from soybean pods as a source of bioactive compounds. The phenolic compound composition of the extract (E) and fraction (F1-F3) as well as their respective lipophilicity were analyzed by UPLC-ESI-QTOF-MS. The biological activity as measured by antioxidant and cytotoxic activity was also evaluated. The in vitro anticancer potential of the ethanolic extract and fraction of soybean pods was measured through MTT assay using human colorectal carcinoma (HTC-116) and prostate cancer (PC-3) cell lines. The F2 fraction, with medium lipophilicity, produced the most pronounced cytotoxic effect in PC-3 cells. Analysis of Pearson’s correlation demonstrated a high relationship between the content of phenolic compounds, the antioxidant activity of the extract and fractions, and cytotoxic activity towards PC-3 cells. UPLC-ESI-QTOF-MS analysis of soybean pod extract resulted in putative identification of 50 polyphenols belonging to three different chemical families (phenolic acids, flavonoids, and other polyphenols), the first time such an analysis has been performed. Among the fractions with variable lipophilicity, fraction F2 was the most abundant in phenolic compounds. This was confirmed in the quantitative analysis, as a result of which the highest total content of phenolic compounds was found in the extract (E) and fraction F2. Soybean pods might be useful material for obtaining preparations with anti-radical and anti-cancer properties. They can also be an active food additive or a component in dietary supplements.Marzena PabichBeata MarciniakRenata KontekMDPI AGarticlesoybean podsantioxidant activitycytotoxic activityphenolic compositionUPLC-ESI-QTOF-MSphenolic compoundsTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10233, p 10233 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	soybean pods antioxidant activity cytotoxic activity phenolic composition UPLC-ESI-QTOF-MS phenolic compounds Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	soybean pods antioxidant activity cytotoxic activity phenolic composition UPLC-ESI-QTOF-MS phenolic compounds Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Marzena Pabich Beata Marciniak Renata Kontek Phenolic Compound Composition and Biological Activities of Fractionated Soybean Pod Extract
description	The objective of this study was to determine the potential of dry extracts from soybean pods as a source of bioactive compounds. The phenolic compound composition of the extract (E) and fraction (F1-F3) as well as their respective lipophilicity were analyzed by UPLC-ESI-QTOF-MS. The biological activity as measured by antioxidant and cytotoxic activity was also evaluated. The in vitro anticancer potential of the ethanolic extract and fraction of soybean pods was measured through MTT assay using human colorectal carcinoma (HTC-116) and prostate cancer (PC-3) cell lines. The F2 fraction, with medium lipophilicity, produced the most pronounced cytotoxic effect in PC-3 cells. Analysis of Pearson’s correlation demonstrated a high relationship between the content of phenolic compounds, the antioxidant activity of the extract and fractions, and cytotoxic activity towards PC-3 cells. UPLC-ESI-QTOF-MS analysis of soybean pod extract resulted in putative identification of 50 polyphenols belonging to three different chemical families (phenolic acids, flavonoids, and other polyphenols), the first time such an analysis has been performed. Among the fractions with variable lipophilicity, fraction F2 was the most abundant in phenolic compounds. This was confirmed in the quantitative analysis, as a result of which the highest total content of phenolic compounds was found in the extract (E) and fraction F2. Soybean pods might be useful material for obtaining preparations with anti-radical and anti-cancer properties. They can also be an active food additive or a component in dietary supplements.
format	article
author	Marzena Pabich Beata Marciniak Renata Kontek
author_facet	Marzena Pabich Beata Marciniak Renata Kontek
author_sort	Marzena Pabich
title	Phenolic Compound Composition and Biological Activities of Fractionated Soybean Pod Extract
title_short	Phenolic Compound Composition and Biological Activities of Fractionated Soybean Pod Extract
title_full	Phenolic Compound Composition and Biological Activities of Fractionated Soybean Pod Extract
title_fullStr	Phenolic Compound Composition and Biological Activities of Fractionated Soybean Pod Extract
title_full_unstemmed	Phenolic Compound Composition and Biological Activities of Fractionated Soybean Pod Extract
title_sort	phenolic compound composition and biological activities of fractionated soybean pod extract
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9b9547dc559a45e485648dfa2bc56253
work_keys_str_mv	AT marzenapabich phenoliccompoundcompositionandbiologicalactivitiesoffractionatedsoybeanpodextract AT beatamarciniak phenoliccompoundcompositionandbiologicalactivitiesoffractionatedsoybeanpodextract AT renatakontek phenoliccompoundcompositionandbiologicalactivitiesoffractionatedsoybeanpodextract
_version_	1718435774354948096

Phenolic Compound Composition and Biological Activities of Fractionated Soybean Pod Extract

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