The mycotoxin deoxynivalenol potentiates intestinal inflammation by Salmonella typhimurium in porcine ileal loops.

The mycotoxin deoxynivalenol potentiates intestinal inflammation by Salmonella typhimurium in porcine ileal loops.

<h4>Background and aims</h4>Both deoxynivalenol (DON) and nontyphoidal salmonellosis are emerging threats with possible hazardous effects on both human and animal health. The objective of this study was to examine whether DON at low but relevant concentrations interacts with the intestin...

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Autores principales: Virginie Vandenbroucke, Siska Croubels, An Martel, Elin Verbrugghe, Joline Goossens, Kim Van Deun, Filip Boyen, Arthur Thompson, Neil Shearer, Patrick De Backer, Freddy Haesebrouck, Frank Pasmans
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Publicado: Public Library of Science (PLoS) 2011
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spelling oai:doaj.org-article:9b99c11d052d4838b094680acba64c782021-11-18T06:46:55ZThe mycotoxin deoxynivalenol potentiates intestinal inflammation by Salmonella typhimurium in porcine ileal loops.1932-620310.1371/journal.pone.0023871https://doaj.org/article/9b99c11d052d4838b094680acba64c782011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21909370/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background and aims</h4>Both deoxynivalenol (DON) and nontyphoidal salmonellosis are emerging threats with possible hazardous effects on both human and animal health. The objective of this study was to examine whether DON at low but relevant concentrations interacts with the intestinal inflammation induced by Salmonella Typhimurium.<h4>Methodology</h4>By using a porcine intestinal ileal loop model, we investigated whether intake of low concentrations of DON interacts with the early intestinal inflammatory response induced by Salmonella Typhimurium.<h4>Results</h4>A significant higher expression of IL-12 and TNFα and a clear potentiation of the expression of IL-1β, IL-8, MCP-1 and IL-6 was seen in loops co-exposed to 1 µg/mL of DON and Salmonella Typhimurium compared to loops exposed to Salmonella Typhimurium alone. This potentiation coincided with a significantly enhanced Salmonella invasion in and translocation over the intestinal epithelial IPEC-J2 cells, exposed to non-cytotoxic concentrations of DON for 24 h. Exposure of Salmonella Typhimurium to 0.250 µg/mL of DON affected the bacterial gene expression level of a limited number of genes, however none of these expression changes seemed to give an explanation for the increased invasion and translocation of Salmonella Typhimurium and the potentiated inflammatory response in combination with DON.<h4>Conclusion</h4>These data imply that the intake of low and relevant concentrations of DON renders the intestinal epithelium more susceptible to Salmonella Typhimurium with a subsequent potentiation of the inflammatory response in the gut.Virginie VandenbrouckeSiska CroubelsAn MartelElin VerbruggheJoline GoossensKim Van DeunFilip BoyenArthur ThompsonNeil ShearerPatrick De BackerFreddy HaesebrouckFrank PasmansPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e23871 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Virginie Vandenbroucke
Siska Croubels
An Martel
Elin Verbrugghe
Joline Goossens
Kim Van Deun
Filip Boyen
Arthur Thompson
Neil Shearer
Patrick De Backer
Freddy Haesebrouck
Frank Pasmans
The mycotoxin deoxynivalenol potentiates intestinal inflammation by Salmonella typhimurium in porcine ileal loops.
description <h4>Background and aims</h4>Both deoxynivalenol (DON) and nontyphoidal salmonellosis are emerging threats with possible hazardous effects on both human and animal health. The objective of this study was to examine whether DON at low but relevant concentrations interacts with the intestinal inflammation induced by Salmonella Typhimurium.<h4>Methodology</h4>By using a porcine intestinal ileal loop model, we investigated whether intake of low concentrations of DON interacts with the early intestinal inflammatory response induced by Salmonella Typhimurium.<h4>Results</h4>A significant higher expression of IL-12 and TNFα and a clear potentiation of the expression of IL-1β, IL-8, MCP-1 and IL-6 was seen in loops co-exposed to 1 µg/mL of DON and Salmonella Typhimurium compared to loops exposed to Salmonella Typhimurium alone. This potentiation coincided with a significantly enhanced Salmonella invasion in and translocation over the intestinal epithelial IPEC-J2 cells, exposed to non-cytotoxic concentrations of DON for 24 h. Exposure of Salmonella Typhimurium to 0.250 µg/mL of DON affected the bacterial gene expression level of a limited number of genes, however none of these expression changes seemed to give an explanation for the increased invasion and translocation of Salmonella Typhimurium and the potentiated inflammatory response in combination with DON.<h4>Conclusion</h4>These data imply that the intake of low and relevant concentrations of DON renders the intestinal epithelium more susceptible to Salmonella Typhimurium with a subsequent potentiation of the inflammatory response in the gut.
format article
author Virginie Vandenbroucke
Siska Croubels
An Martel
Elin Verbrugghe
Joline Goossens
Kim Van Deun
Filip Boyen
Arthur Thompson
Neil Shearer
Patrick De Backer
Freddy Haesebrouck
Frank Pasmans
author_facet Virginie Vandenbroucke
Siska Croubels
An Martel
Elin Verbrugghe
Joline Goossens
Kim Van Deun
Filip Boyen
Arthur Thompson
Neil Shearer
Patrick De Backer
Freddy Haesebrouck
Frank Pasmans
author_sort Virginie Vandenbroucke
title The mycotoxin deoxynivalenol potentiates intestinal inflammation by Salmonella typhimurium in porcine ileal loops.
title_short The mycotoxin deoxynivalenol potentiates intestinal inflammation by Salmonella typhimurium in porcine ileal loops.
title_full The mycotoxin deoxynivalenol potentiates intestinal inflammation by Salmonella typhimurium in porcine ileal loops.
title_fullStr The mycotoxin deoxynivalenol potentiates intestinal inflammation by Salmonella typhimurium in porcine ileal loops.
title_full_unstemmed The mycotoxin deoxynivalenol potentiates intestinal inflammation by Salmonella typhimurium in porcine ileal loops.
title_sort mycotoxin deoxynivalenol potentiates intestinal inflammation by salmonella typhimurium in porcine ileal loops.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/9b99c11d052d4838b094680acba64c78
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