The interactions of single-wall carbon nanohorns with polar epithelium

Yujie Shi,1 Zujin Shi,2 Suxin Li,1 Yuan Zhang,3 Bing He,1 Dong Peng,4 Jie Tian,4 Ming Zhao,5 Xueqing Wang,1 Qiang Zhang1 1Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, School of Pharmaceutical Sciences, 2Beijing National Laboratory for Molecular Sciences, State Ke...

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Autores principales:	Shi YJ, Shi ZJ, Li SX, Zhang Y, He B, Peng D, Tian J, Zhao M, Wang XQ, Zhang Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	carbon nanohorns epithelial cells transport mechanisms nanocarriers MSOT imaging Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9b9a12fa33cd44268f26f2e3875e9f0a
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spelling	oai:doaj.org-article:9b9a12fa33cd44268f26f2e3875e9f0a2021-12-02T00:37:57ZThe interactions of single-wall carbon nanohorns with polar epithelium1178-2013https://doaj.org/article/9b9a12fa33cd44268f26f2e3875e9f0a2017-06-01T00:00:00Zhttps://www.dovepress.com/the-interactions-of-single-wall-carbon-nanohorns-with-polar-epithelium-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yujie Shi,1 Zujin Shi,2 Suxin Li,1 Yuan Zhang,3 Bing He,1 Dong Peng,4 Jie Tian,4 Ming Zhao,5 Xueqing Wang,1 Qiang Zhang1 1Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, School of Pharmaceutical Sciences, 2Beijing National Laboratory for Molecular Sciences, State Key Lab of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing, People’s Republic of China; 3Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI, USA; 4Key Laboratory of Molecular Imaging of Chinese Academy of Sciences, Institute of Automation, Chinese Academy of Sciences, 5Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing, People’s Republic of China Abstract: Single-wall carbon nanohorns (SWCNHs), which have multitudes of horn interstices, an extensive surface area, and a spherical aggregate structure, offer many advantages over other carbon nanomaterials being used as a drug nanovector. The previous studies on the interaction between SWCNHs and cells have mostly emphasized on cellular uptake and intracellular trafficking, but seldom on epithelial cells. Polar epithelium as a typical biological barrier constitutes the prime obstacle for the transport of therapeutic agents to target site. This work tried to explore the permeability of SWCNHs through polar epithelium and their abilities to modulate transcellular transport, and evaluate the potential of SWCNHs in drug delivery. Madin-Darby canine kidney (MDCK) cell monolayer was used as a polar epithelial cell model, and as-grown SWCNHs, together with oxidized and fluorescein isothiocyanate-conjugated bovine serum albumin-labeled forms, were constructed and comprehensively investigated in vitro and in vivo. Various methods such as transmission electron microscopy and confocal imaging were used to visualize their intracellular uptake and localization, as well as to investigate the potential transcytotic process. The related mechanism was explored by specific inhibitors. Additionally, fast multispectral optoacoustic tomography imaging was used for monitoring the distribution and transport process of SWCNHs in vivo after oral administration in nude mice, as an evidence for their interaction with the intestinal epithelium. The results showed that SWCNHs had a strong bioadhesion property, and parts of them could be uptaken and transcytosed across the MDCK monolayer. Multiple mechanisms were involved in the uptake and transcytosis of SWCNHs with varying degrees. After oral administration, oxidized SWCNHs were distributed in the gastrointestinal tract and retained in the intestine for up to 36 h probably due to their surface adhesion and endocytosis into the intestinal epithelium. Overall, this comprehensive investigation demonstrated that SWCNHs can serve as a promising nanovector that can cross the barrier of polar epithelial cells and deliver drugs effectively. Keywords: carbon nanohorns, epithelial cells, transport mechanisms, nanocarriers, MSOT imagingShi YJShi ZJLi SXZhang YHe BPeng DTian JZhao MWang XQZhang QDove Medical Pressarticlecarbon nanohornsepithelial cellstransport mechanismsnanocarriersMSOT imagingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 4177-4194 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbon nanohorns epithelial cells transport mechanisms nanocarriers MSOT imaging Medicine (General) R5-920
spellingShingle	carbon nanohorns epithelial cells transport mechanisms nanocarriers MSOT imaging Medicine (General) R5-920 Shi YJ Shi ZJ Li SX Zhang Y He B Peng D Tian J Zhao M Wang XQ Zhang Q The interactions of single-wall carbon nanohorns with polar epithelium
description	Yujie Shi,1 Zujin Shi,2 Suxin Li,1 Yuan Zhang,3 Bing He,1 Dong Peng,4 Jie Tian,4 Ming Zhao,5 Xueqing Wang,1 Qiang Zhang1 1Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, School of Pharmaceutical Sciences, 2Beijing National Laboratory for Molecular Sciences, State Key Lab of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing, People’s Republic of China; 3Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI, USA; 4Key Laboratory of Molecular Imaging of Chinese Academy of Sciences, Institute of Automation, Chinese Academy of Sciences, 5Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing, People’s Republic of China Abstract: Single-wall carbon nanohorns (SWCNHs), which have multitudes of horn interstices, an extensive surface area, and a spherical aggregate structure, offer many advantages over other carbon nanomaterials being used as a drug nanovector. The previous studies on the interaction between SWCNHs and cells have mostly emphasized on cellular uptake and intracellular trafficking, but seldom on epithelial cells. Polar epithelium as a typical biological barrier constitutes the prime obstacle for the transport of therapeutic agents to target site. This work tried to explore the permeability of SWCNHs through polar epithelium and their abilities to modulate transcellular transport, and evaluate the potential of SWCNHs in drug delivery. Madin-Darby canine kidney (MDCK) cell monolayer was used as a polar epithelial cell model, and as-grown SWCNHs, together with oxidized and fluorescein isothiocyanate-conjugated bovine serum albumin-labeled forms, were constructed and comprehensively investigated in vitro and in vivo. Various methods such as transmission electron microscopy and confocal imaging were used to visualize their intracellular uptake and localization, as well as to investigate the potential transcytotic process. The related mechanism was explored by specific inhibitors. Additionally, fast multispectral optoacoustic tomography imaging was used for monitoring the distribution and transport process of SWCNHs in vivo after oral administration in nude mice, as an evidence for their interaction with the intestinal epithelium. The results showed that SWCNHs had a strong bioadhesion property, and parts of them could be uptaken and transcytosed across the MDCK monolayer. Multiple mechanisms were involved in the uptake and transcytosis of SWCNHs with varying degrees. After oral administration, oxidized SWCNHs were distributed in the gastrointestinal tract and retained in the intestine for up to 36 h probably due to their surface adhesion and endocytosis into the intestinal epithelium. Overall, this comprehensive investigation demonstrated that SWCNHs can serve as a promising nanovector that can cross the barrier of polar epithelial cells and deliver drugs effectively. Keywords: carbon nanohorns, epithelial cells, transport mechanisms, nanocarriers, MSOT imaging
format	article
author	Shi YJ Shi ZJ Li SX Zhang Y He B Peng D Tian J Zhao M Wang XQ Zhang Q
author_facet	Shi YJ Shi ZJ Li SX Zhang Y He B Peng D Tian J Zhao M Wang XQ Zhang Q
author_sort	Shi YJ
title	The interactions of single-wall carbon nanohorns with polar epithelium
title_short	The interactions of single-wall carbon nanohorns with polar epithelium
title_full	The interactions of single-wall carbon nanohorns with polar epithelium
title_fullStr	The interactions of single-wall carbon nanohorns with polar epithelium
title_full_unstemmed	The interactions of single-wall carbon nanohorns with polar epithelium
title_sort	interactions of single-wall carbon nanohorns with polar epithelium
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/9b9a12fa33cd44268f26f2e3875e9f0a
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The interactions of single-wall carbon nanohorns with polar epithelium

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