Rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip

In this paper, we describe the development of a rapid, simple, specific, and sensitive immunochromatographic strip test based on an antibody–antigen reaction for detection of tulathromycin (TULA) in samples of 0.01 M PBS pH 7.4, pure milk and honey. The monoclonal antibodies specifically recognized...

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Autores principales:	Liqiang Liu, Liguang Xu, Steven Suryoprabowo, Shanshan Song, Hua Kuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	immunochromatographic assay monoclonal antibody test strip tulathromycin Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/9b9b03401e344985a1bfa57a5c2ddc3f
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spelling	oai:doaj.org-article:9b9b03401e344985a1bfa57a5c2ddc3f2021-11-26T11:19:46ZRapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip0954-01051465-344310.1080/09540105.2017.1376040https://doaj.org/article/9b9b03401e344985a1bfa57a5c2ddc3f2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1376040https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443In this paper, we describe the development of a rapid, simple, specific, and sensitive immunochromatographic strip test based on an antibody–antigen reaction for detection of tulathromycin (TULA) in samples of 0.01 M PBS pH 7.4, pure milk and honey. The monoclonal antibodies specifically recognized the corresponding antigens produced in our laboratory. Under optimized conditions, the cut-off limits of the test strips for TULA in 0.01 M PBS pH 7.4 was found to be 2.5 ng/mL. Meanwhile, with simple preparation for detection in pure milk and honey samples, the cut-off limits for TULA were found to be 5 and 10 ng/mL, respectively. Each test can be evaluated in 5 min. In summary, this lateral-flow device provides a sensitive, effective, specific, and rapid method for detection of TULA residues in food matrix.Liqiang LiuLiguang XuSteven SuryoprabowoShanshan SongHua KuangTaylor & Francis Grouparticleimmunochromatographic assaymonoclonal antibodytest striptulathromycinAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 358-368 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	immunochromatographic assay monoclonal antibody test strip tulathromycin Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	immunochromatographic assay monoclonal antibody test strip tulathromycin Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Liqiang Liu Liguang Xu Steven Suryoprabowo Shanshan Song Hua Kuang Rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip
description	In this paper, we describe the development of a rapid, simple, specific, and sensitive immunochromatographic strip test based on an antibody–antigen reaction for detection of tulathromycin (TULA) in samples of 0.01 M PBS pH 7.4, pure milk and honey. The monoclonal antibodies specifically recognized the corresponding antigens produced in our laboratory. Under optimized conditions, the cut-off limits of the test strips for TULA in 0.01 M PBS pH 7.4 was found to be 2.5 ng/mL. Meanwhile, with simple preparation for detection in pure milk and honey samples, the cut-off limits for TULA were found to be 5 and 10 ng/mL, respectively. Each test can be evaluated in 5 min. In summary, this lateral-flow device provides a sensitive, effective, specific, and rapid method for detection of TULA residues in food matrix.
format	article
author	Liqiang Liu Liguang Xu Steven Suryoprabowo Shanshan Song Hua Kuang
author_facet	Liqiang Liu Liguang Xu Steven Suryoprabowo Shanshan Song Hua Kuang
author_sort	Liqiang Liu
title	Rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip
title_short	Rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip
title_full	Rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip
title_fullStr	Rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip
title_full_unstemmed	Rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip
title_sort	rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/9b9b03401e344985a1bfa57a5c2ddc3f
work_keys_str_mv	AT liqiangliu rapiddetectionoftulathromycininpuremilkandhoneywithanimmunochromatographicteststrip AT liguangxu rapiddetectionoftulathromycininpuremilkandhoneywithanimmunochromatographicteststrip AT stevensuryoprabowo rapiddetectionoftulathromycininpuremilkandhoneywithanimmunochromatographicteststrip AT shanshansong rapiddetectionoftulathromycininpuremilkandhoneywithanimmunochromatographicteststrip AT huakuang rapiddetectionoftulathromycininpuremilkandhoneywithanimmunochromatographicteststrip
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Rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip

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