Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack

Background: Plant tissue culture involves the use of explants obtained from plants to induce organogenesis with the help of plant growth regulators (PGRs). Micropropagation techniques provide a faster and economical solution to the limitations associated with traditional methods of plant cultivation...

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Autores principales:	Ankita Rajendra Parab, Bee Lynn Chew, Lit Chow Yeow, Sreeramanan Subramaniam
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Apical buds Cytokinins Ficus carica var. Black Jack. Ficus carica Micropropagation Multiple shoot induction Biotechnology TP248.13-248.65 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9b9e07aa19b043c58c56b21a3464def6
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spelling	oai:doaj.org-article:9b9e07aa19b043c58c56b21a3464def62021-11-06T04:20:44ZOrganogenesis on apical buds in common fig (Ficus carica) var. Black Jack0717-345810.1016/j.ejbt.2021.10.001https://doaj.org/article/9b9e07aa19b043c58c56b21a3464def62021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0717345821000488https://doaj.org/toc/0717-3458Background: Plant tissue culture involves the use of explants obtained from plants to induce organogenesis with the help of plant growth regulators (PGRs). Micropropagation techniques provide a faster and economical solution to the limitations associated with traditional methods of plant cultivation. The present study focuses on the multiple shoot induction and proliferation of Ficus carica var. Black Jack. Factors that influence the growth of in vitro multiple shoots on the apical buds, which include growth media and PGRs, were investigated in this study. Different concentrations of cytokinins like 6-benzylaminopurine (BAP), Thidiazuron (TDZ), and Kinetin (Kin) were used on woody plant medium (WPM) for the optimization of media for multiple shoot induction and proliferation. Results: Apical buds of Ficus carica var. Black Jack growing in WPM supplemented with BAP produced the healthiest plantlets, with the highest number of multiple shoots. The most efficient medium composition which produced the highest number of multiple shoots (37.8) per growing explant was WPM supplemented with 20 µM BAP. Proliferated multiple shoots were efficiently rooted using WPM + 20 µM BAP + 8 µM indole-3-acetic acid (IAA). This optimized medium composition significantly enhanced the production of multiple, disease-free plantlets using single apical bud explants of Ficus carica var. Black Jack. Conclusions: In the present study the observations indicate that WPM supplemented with 20 µM BAP is the best-suited medium for organogenesis and multiple shoot culture of Ficus carica var. Black Jack, and this technique can be potentially applied for commercialization of the plant.How to cite: Parab AR, Chew BL, Yeow LC, et al. Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2020.01.010Ankita Rajendra ParabBee Lynn ChewLit Chow YeowSreeramanan SubramaniamElsevierarticleApical budsCytokininsFicus carica var. Black Jack.Ficus caricaMicropropagationMultiple shoot inductionBiotechnologyTP248.13-248.65Biology (General)QH301-705.5ENElectronic Journal of Biotechnology, Vol 54, Iss , Pp 69-76 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Apical buds Cytokinins Ficus carica var. Black Jack. Ficus carica Micropropagation Multiple shoot induction Biotechnology TP248.13-248.65 Biology (General) QH301-705.5
spellingShingle	Apical buds Cytokinins Ficus carica var. Black Jack. Ficus carica Micropropagation Multiple shoot induction Biotechnology TP248.13-248.65 Biology (General) QH301-705.5 Ankita Rajendra Parab Bee Lynn Chew Lit Chow Yeow Sreeramanan Subramaniam Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack
description	Background: Plant tissue culture involves the use of explants obtained from plants to induce organogenesis with the help of plant growth regulators (PGRs). Micropropagation techniques provide a faster and economical solution to the limitations associated with traditional methods of plant cultivation. The present study focuses on the multiple shoot induction and proliferation of Ficus carica var. Black Jack. Factors that influence the growth of in vitro multiple shoots on the apical buds, which include growth media and PGRs, were investigated in this study. Different concentrations of cytokinins like 6-benzylaminopurine (BAP), Thidiazuron (TDZ), and Kinetin (Kin) were used on woody plant medium (WPM) for the optimization of media for multiple shoot induction and proliferation. Results: Apical buds of Ficus carica var. Black Jack growing in WPM supplemented with BAP produced the healthiest plantlets, with the highest number of multiple shoots. The most efficient medium composition which produced the highest number of multiple shoots (37.8) per growing explant was WPM supplemented with 20 µM BAP. Proliferated multiple shoots were efficiently rooted using WPM + 20 µM BAP + 8 µM indole-3-acetic acid (IAA). This optimized medium composition significantly enhanced the production of multiple, disease-free plantlets using single apical bud explants of Ficus carica var. Black Jack. Conclusions: In the present study the observations indicate that WPM supplemented with 20 µM BAP is the best-suited medium for organogenesis and multiple shoot culture of Ficus carica var. Black Jack, and this technique can be potentially applied for commercialization of the plant.How to cite: Parab AR, Chew BL, Yeow LC, et al. Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2020.01.010
format	article
author	Ankita Rajendra Parab Bee Lynn Chew Lit Chow Yeow Sreeramanan Subramaniam
author_facet	Ankita Rajendra Parab Bee Lynn Chew Lit Chow Yeow Sreeramanan Subramaniam
author_sort	Ankita Rajendra Parab
title	Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack
title_short	Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack
title_full	Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack
title_fullStr	Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack
title_full_unstemmed	Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack
title_sort	organogenesis on apical buds in common fig (ficus carica) var. black jack
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/9b9e07aa19b043c58c56b21a3464def6
work_keys_str_mv	AT ankitarajendraparab organogenesisonapicalbudsincommonfigficuscaricavarblackjack AT beelynnchew organogenesisonapicalbudsincommonfigficuscaricavarblackjack AT litchowyeow organogenesisonapicalbudsincommonfigficuscaricavarblackjack AT sreeramanansubramaniam organogenesisonapicalbudsincommonfigficuscaricavarblackjack
_version_	1718443932457631744

Organogenesis on apical buds in common fig (Ficus carica) var. Black Jack

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