Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Abstract We aimed to isolate Acinetobacter baumannii (A. baumannii) from wound infections, determine their resistance and virulence profile, and assess the impact of Silver nanoparticles (AgNPs) on the bacterial growth, virulence and biofilm-related gene expression. AgNPs were synthesized and charac...

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Autores principales:	Helal F. Hetta, Israa M. S. Al-Kadmy, Saba Saadoon Khazaal, Suhad Abbas, Ahmed Suhail, Mohamed A. El-Mokhtar, Noura H. Abd Ellah, Esraa A. Ahmed, Rasha B. Abd-ellatief, Eman A. El-Masry, Gaber El-Saber Batiha, Azza A. Elkady, Nahed A. Mohamed, Abdelazeem M. Algammal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9ba6ee0ed2ad4962a9e40a48c316f855
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spelling	oai:doaj.org-article:9ba6ee0ed2ad4962a9e40a48c316f8552021-12-02T15:00:25ZAntibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii10.1038/s41598-021-90208-42045-2322https://doaj.org/article/9ba6ee0ed2ad4962a9e40a48c316f8552021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90208-4https://doaj.org/toc/2045-2322Abstract We aimed to isolate Acinetobacter baumannii (A. baumannii) from wound infections, determine their resistance and virulence profile, and assess the impact of Silver nanoparticles (AgNPs) on the bacterial growth, virulence and biofilm-related gene expression. AgNPs were synthesized and characterized using TEM, XRD and FTIR spectroscopy. A. baumannii (n = 200) were isolated and identified. Resistance pattern was determined and virulence genes (afa/draBC, cnf1, cnf2, csgA, cvaC, fimH, fyuA, ibeA, iutA, kpsMT II, PAI, papC, PapG II, III, sfa/focDE and traT) were screened using PCR. Biofilm formation was evaluated using Microtiter plate method. Then, the antimicrobial activity of AgNPs was evaluated by the well-diffusion method, growth kinetics and MIC determination. Inhibition of biofilm formation and the ability to disperse biofilms in exposure to AgNPs were evaluated. The effect of AgNPs on the expression of virulence and biofilm-related genes (bap, OmpA, abaI, csuA/B, A1S_2091, A1S_1510, A1S_0690, A1S_0114) were estimated using QRT-PCR. In vitro infection model for analyzing the antibacterial activity of AgNPs was done using a co-culture infection model of A. baumannii with human fibroblast skin cell line HFF-1 or Vero cell lines. A. baumannii had high level of resistance to antibiotics. Most of the isolates harbored the fimH, afa/draBC, cnf1, csgA and cnf2, and the majority of A. baumannii produced strong biofilms. AgNPs inhibited the growth of A. baumannii efficiently with MIC ranging from 4 to 25 µg/ml. A. baumannii showed a reduced growth rate in the presence of AgNPs. The inhibitory activity and the anti-biofilm activity of AgNPs were more pronounced against the weak biofilm producers. Moreover, AgNPs decreased the expression of kpsMII , afa/draBC,bap, OmpA, and csuA/B genes. The in vitro infection model revealed a significant antibacterial activity of AgNPs against extracellular and intracellular A. baumannii. AgNPs highly interrupted bacterial multiplication and biofilm formation. AgNPs downregulated the transcription level of important virulence and biofilm-related genes. Our findings provide an additional step towards understanding the mechanisms by which sliver nanoparticles interfere with the microbial spread and persistence.Helal F. HettaIsraa M. S. Al-KadmySaba Saadoon KhazaalSuhad AbbasAhmed SuhailMohamed A. El-MokhtarNoura H. Abd EllahEsraa A. AhmedRasha B. Abd-ellatiefEman A. El-MasryGaber El-Saber BatihaAzza A. ElkadyNahed A. MohamedAbdelazeem M. AlgammalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Helal F. Hetta Israa M. S. Al-Kadmy Saba Saadoon Khazaal Suhad Abbas Ahmed Suhail Mohamed A. El-Mokhtar Noura H. Abd Ellah Esraa A. Ahmed Rasha B. Abd-ellatief Eman A. El-Masry Gaber El-Saber Batiha Azza A. Elkady Nahed A. Mohamed Abdelazeem M. Algammal Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii
description	Abstract We aimed to isolate Acinetobacter baumannii (A. baumannii) from wound infections, determine their resistance and virulence profile, and assess the impact of Silver nanoparticles (AgNPs) on the bacterial growth, virulence and biofilm-related gene expression. AgNPs were synthesized and characterized using TEM, XRD and FTIR spectroscopy. A. baumannii (n = 200) were isolated and identified. Resistance pattern was determined and virulence genes (afa/draBC, cnf1, cnf2, csgA, cvaC, fimH, fyuA, ibeA, iutA, kpsMT II, PAI, papC, PapG II, III, sfa/focDE and traT) were screened using PCR. Biofilm formation was evaluated using Microtiter plate method. Then, the antimicrobial activity of AgNPs was evaluated by the well-diffusion method, growth kinetics and MIC determination. Inhibition of biofilm formation and the ability to disperse biofilms in exposure to AgNPs were evaluated. The effect of AgNPs on the expression of virulence and biofilm-related genes (bap, OmpA, abaI, csuA/B, A1S_2091, A1S_1510, A1S_0690, A1S_0114) were estimated using QRT-PCR. In vitro infection model for analyzing the antibacterial activity of AgNPs was done using a co-culture infection model of A. baumannii with human fibroblast skin cell line HFF-1 or Vero cell lines. A. baumannii had high level of resistance to antibiotics. Most of the isolates harbored the fimH, afa/draBC, cnf1, csgA and cnf2, and the majority of A. baumannii produced strong biofilms. AgNPs inhibited the growth of A. baumannii efficiently with MIC ranging from 4 to 25 µg/ml. A. baumannii showed a reduced growth rate in the presence of AgNPs. The inhibitory activity and the anti-biofilm activity of AgNPs were more pronounced against the weak biofilm producers. Moreover, AgNPs decreased the expression of kpsMII , afa/draBC,bap, OmpA, and csuA/B genes. The in vitro infection model revealed a significant antibacterial activity of AgNPs against extracellular and intracellular A. baumannii. AgNPs highly interrupted bacterial multiplication and biofilm formation. AgNPs downregulated the transcription level of important virulence and biofilm-related genes. Our findings provide an additional step towards understanding the mechanisms by which sliver nanoparticles interfere with the microbial spread and persistence.
format	article
author	Helal F. Hetta Israa M. S. Al-Kadmy Saba Saadoon Khazaal Suhad Abbas Ahmed Suhail Mohamed A. El-Mokhtar Noura H. Abd Ellah Esraa A. Ahmed Rasha B. Abd-ellatief Eman A. El-Masry Gaber El-Saber Batiha Azza A. Elkady Nahed A. Mohamed Abdelazeem M. Algammal
author_facet	Helal F. Hetta Israa M. S. Al-Kadmy Saba Saadoon Khazaal Suhad Abbas Ahmed Suhail Mohamed A. El-Mokhtar Noura H. Abd Ellah Esraa A. Ahmed Rasha B. Abd-ellatief Eman A. El-Masry Gaber El-Saber Batiha Azza A. Elkady Nahed A. Mohamed Abdelazeem M. Algammal
author_sort	Helal F. Hetta
title	Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii
title_short	Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii
title_full	Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii
title_fullStr	Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii
title_full_unstemmed	Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii
title_sort	antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant acinetobacter baumannii
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9ba6ee0ed2ad4962a9e40a48c316f855
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Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

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