EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE

Abstract. The purpose of this article was to make a broad audience of experts familiar to new developments in the method of flow cytometry that have found applications in medical and biologic studies, in order of their further implementation into everyday practice. These applications include the fol...

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Autores principales:	S. V. Khaidukov, A. V. Zurochka
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	flow cytometry tetramer technology basophils cytokines multiparametric analysis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/9ba72e9cba3c40428bfac6d8d8c3023e
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spelling	oai:doaj.org-article:9ba72e9cba3c40428bfac6d8d8c3023e2021-11-18T08:03:37ZEXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE1563-06252313-741X10.15789/1563-0625-2008-1-5-12https://doaj.org/article/9ba72e9cba3c40428bfac6d8d8c3023e2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/70https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. The purpose of this article was to make a broad audience of experts familiar to new developments in the method of flow cytometry that have found applications in medical and biologic studies, in order of their further implementation into everyday practice. These applications include the following approcahes: detection of antigen-specific T-cells by using of tetramer technology, flow cytometric determination of cytokines in biological liquids, determination of in vitro sensitivity of basophilic granulocytes to allergen effects, detection of Th1 and Т-reg cells by their cell surface markers. When using multiparametric analysis, a multi-step gating and other new technologies, the flow cytometry technique allows of location and tracing the majority of processes occurring in development of immune response. When studying these dynamic events, we get an opportunity to react adequately to apropriate changes, and to develop new approaches to correct altered cellular activities, that they should determinate.S. V. KhaidukovA. V. ZurochkaSPb RAACIarticleflow cytometrytetramer technologybasophilscytokinesmultiparametric analysisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 10, Iss 1, Pp 5-12 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	flow cytometry tetramer technology basophils cytokines multiparametric analysis Immunologic diseases. Allergy RC581-607
spellingShingle	flow cytometry tetramer technology basophils cytokines multiparametric analysis Immunologic diseases. Allergy RC581-607 S. V. Khaidukov A. V. Zurochka EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE
description	Abstract. The purpose of this article was to make a broad audience of experts familiar to new developments in the method of flow cytometry that have found applications in medical and biologic studies, in order of their further implementation into everyday practice. These applications include the following approcahes: detection of antigen-specific T-cells by using of tetramer technology, flow cytometric determination of cytokines in biological liquids, determination of in vitro sensitivity of basophilic granulocytes to allergen effects, detection of Th1 and Т-reg cells by their cell surface markers. When using multiparametric analysis, a multi-step gating and other new technologies, the flow cytometry technique allows of location and tracing the majority of processes occurring in development of immune response. When studying these dynamic events, we get an opportunity to react adequately to apropriate changes, and to develop new approaches to correct altered cellular activities, that they should determinate.
format	article
author	S. V. Khaidukov A. V. Zurochka
author_facet	S. V. Khaidukov A. V. Zurochka
author_sort	S. V. Khaidukov
title	EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE
title_short	EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE
title_full	EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE
title_fullStr	EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE
title_full_unstemmed	EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE
title_sort	expansion of opportunities of the flow cytometry method for clinical and immunologycal practice
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/9ba72e9cba3c40428bfac6d8d8c3023e
work_keys_str_mv	AT svkhaidukov expansionofopportunitiesoftheflowcytometrymethodforclinicalandimmunologycalpractice AT avzurochka expansionofopportunitiesoftheflowcytometrymethodforclinicalandimmunologycalpractice
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EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE

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