Negatively Regulated by miR-29c-3p, MTFR1 Promotes the Progression and Glycolysis in Lung Adenocarcinoma via the AMPK/mTOR Signalling Pathway

Background: Lung adenocarcinoma (LUAD) is the major form of lung cancer that presents a major peril to public health. Owing to the high rates of morbidity, mortality and chemoresistance, it is necessary to develop more effective therapeutic targets of LUAD. Mitochondrial fission regulator 1 (MTFR1)...

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Autores principales:	Yongmeng Li, Yanfei Liu, Kai Jin, Rui Dong, Cun Gao, Libo Si, Zitong Feng, Huiying Zhang, Hui Tian
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	lung cancer microRNA mitochondrial fission regulator 1 Warburg effect proliferation invasion Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9badcc4e38704b4abec3618eded133c8
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spelling	oai:doaj.org-article:9badcc4e38704b4abec3618eded133c82021-12-02T00:36:30ZNegatively Regulated by miR-29c-3p, MTFR1 Promotes the Progression and Glycolysis in Lung Adenocarcinoma via the AMPK/mTOR Signalling Pathway2296-634X10.3389/fcell.2021.771824https://doaj.org/article/9badcc4e38704b4abec3618eded133c82021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.771824/fullhttps://doaj.org/toc/2296-634XBackground: Lung adenocarcinoma (LUAD) is the major form of lung cancer that presents a major peril to public health. Owing to the high rates of morbidity, mortality and chemoresistance, it is necessary to develop more effective therapeutic targets of LUAD. Mitochondrial fission regulator 1 (MTFR1) affects the occurrence and development of some diseases by regulating mitochondrial dynamics and is dysregulated in LUAD. However, the functions and molecular mechanisms of MTFR1 in LUAD have not been investigated.Methods: Immunohistochemical (IHC) analysis, real-time quantitative polymerase chain reaction (RT-qPCR), bioinformatic analysis and western blot (WB) were performed to assess the expression of MTFR1 at both protein and mRNA levels. The biological functions of MTFR1 in LUAD cells were assessed based on various in vivo and in vitro experiments. The dual-luciferase reporter assay and some rescue experiments were performed to evaluate the underlying mechanism of MTFR1 in LUAD.Results: MTFR1 was upregulated in LUAD cells and tissues and correlated with dismal clinicopathologic features and a worse prognosis of patients with LUAD. Functionally, MTFR1 overexpression stimulated the proliferation, invasion, migration and glycolytic capacity and impeded the apoptosis of LUAD cells; however, opposite results were obtained when MTFR1 expression was knocked down. MTFR1, which was directly targeted by miR-29c-3p, may exert its biological functions through the AMPK/mTOR signalling pathway.Conclusion: MTFR1 promotes the progression of LUAD. Therefore, targeting MTFR1 can offer an effective therapeutic strategy for LUAD treatment.Yongmeng LiYanfei LiuKai JinRui DongCun GaoLibo SiZitong FengHuiying ZhangHui TianFrontiers Media S.A.articlelung cancermicroRNAmitochondrial fission regulator 1Warburg effectproliferationinvasionBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lung cancer microRNA mitochondrial fission regulator 1 Warburg effect proliferation invasion Biology (General) QH301-705.5
spellingShingle	lung cancer microRNA mitochondrial fission regulator 1 Warburg effect proliferation invasion Biology (General) QH301-705.5 Yongmeng Li Yanfei Liu Kai Jin Rui Dong Cun Gao Libo Si Zitong Feng Huiying Zhang Hui Tian Negatively Regulated by miR-29c-3p, MTFR1 Promotes the Progression and Glycolysis in Lung Adenocarcinoma via the AMPK/mTOR Signalling Pathway
description	Background: Lung adenocarcinoma (LUAD) is the major form of lung cancer that presents a major peril to public health. Owing to the high rates of morbidity, mortality and chemoresistance, it is necessary to develop more effective therapeutic targets of LUAD. Mitochondrial fission regulator 1 (MTFR1) affects the occurrence and development of some diseases by regulating mitochondrial dynamics and is dysregulated in LUAD. However, the functions and molecular mechanisms of MTFR1 in LUAD have not been investigated.Methods: Immunohistochemical (IHC) analysis, real-time quantitative polymerase chain reaction (RT-qPCR), bioinformatic analysis and western blot (WB) were performed to assess the expression of MTFR1 at both protein and mRNA levels. The biological functions of MTFR1 in LUAD cells were assessed based on various in vivo and in vitro experiments. The dual-luciferase reporter assay and some rescue experiments were performed to evaluate the underlying mechanism of MTFR1 in LUAD.Results: MTFR1 was upregulated in LUAD cells and tissues and correlated with dismal clinicopathologic features and a worse prognosis of patients with LUAD. Functionally, MTFR1 overexpression stimulated the proliferation, invasion, migration and glycolytic capacity and impeded the apoptosis of LUAD cells; however, opposite results were obtained when MTFR1 expression was knocked down. MTFR1, which was directly targeted by miR-29c-3p, may exert its biological functions through the AMPK/mTOR signalling pathway.Conclusion: MTFR1 promotes the progression of LUAD. Therefore, targeting MTFR1 can offer an effective therapeutic strategy for LUAD treatment.
format	article
author	Yongmeng Li Yanfei Liu Kai Jin Rui Dong Cun Gao Libo Si Zitong Feng Huiying Zhang Hui Tian
author_facet	Yongmeng Li Yanfei Liu Kai Jin Rui Dong Cun Gao Libo Si Zitong Feng Huiying Zhang Hui Tian
author_sort	Yongmeng Li
title	Negatively Regulated by miR-29c-3p, MTFR1 Promotes the Progression and Glycolysis in Lung Adenocarcinoma via the AMPK/mTOR Signalling Pathway
title_short	Negatively Regulated by miR-29c-3p, MTFR1 Promotes the Progression and Glycolysis in Lung Adenocarcinoma via the AMPK/mTOR Signalling Pathway
title_full	Negatively Regulated by miR-29c-3p, MTFR1 Promotes the Progression and Glycolysis in Lung Adenocarcinoma via the AMPK/mTOR Signalling Pathway
title_fullStr	Negatively Regulated by miR-29c-3p, MTFR1 Promotes the Progression and Glycolysis in Lung Adenocarcinoma via the AMPK/mTOR Signalling Pathway
title_full_unstemmed	Negatively Regulated by miR-29c-3p, MTFR1 Promotes the Progression and Glycolysis in Lung Adenocarcinoma via the AMPK/mTOR Signalling Pathway
title_sort	negatively regulated by mir-29c-3p, mtfr1 promotes the progression and glycolysis in lung adenocarcinoma via the ampk/mtor signalling pathway
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/9badcc4e38704b4abec3618eded133c8
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Negatively Regulated by miR-29c-3p, MTFR1 Promotes the Progression and Glycolysis in Lung Adenocarcinoma via the AMPK/mTOR Signalling Pathway

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