Changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion
Abstract After spinal cord injury (SCI), patients face many physical and psychological issues including intestinal dysfunction and comorbidities, strongly affecting quality of life. The gut microbiota has recently been suggested to influence the course of the disease in these patients. However, to d...
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Nature Portfolio
2021
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oai:doaj.org-article:9bb5424d0eb64cb2876bbe61a76285952021-12-02T16:04:13ZChanges in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion10.1038/s41598-021-92027-z2045-2322https://doaj.org/article/9bb5424d0eb64cb2876bbe61a76285952021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92027-zhttps://doaj.org/toc/2045-2322Abstract After spinal cord injury (SCI), patients face many physical and psychological issues including intestinal dysfunction and comorbidities, strongly affecting quality of life. The gut microbiota has recently been suggested to influence the course of the disease in these patients. However, to date only two studies have profiled the gut microbiota in SCI patients, months after a traumatic injury. Here we characterized the gut microbiota in a large Italian SCI population, within a short time from a not only traumatic injury. Feces were collected within the first week at the rehabilitation center (no later than 60 days after SCI), and profiled by 16S rRNA gene-based next-generation sequencing. Microbial profiles were compared to those publicly available of healthy age- and gender-matched Italians, and correlated to patient metadata, including type of SCI, spinal unit location, nutrition and concomitant antibiotic therapies. The gut microbiota of SCI patients shows distinct dysbiotic signatures, i.e. increase in potentially pathogenic, pro-inflammatory and mucus-degrading bacteria, and depletion of short-chain fatty acid producers. While robust to most host variables, such dysbiosis varies by lesion level and completeness, with the most neurologically impaired patients showing an even more unbalanced microbial profile. The SCI-related gut microbiome dysbiosis is very likely secondary to injury and closely related to the degree of completeness and severity of the lesion, regardless of etiology and time interval. This microbial layout could variously contribute to increased gut permeability and inflammation, potentially predisposing patients to the onset of severe comorbidities.Gabriele BazzocchiSilvia TurroniMaria Chiara BulzaminiFederica D’AmicoAngelica BavaMirco CastiglioniValentina CagnettaErnesto LosavioMaurizio CazzanigaLaura TerenghiLuisa De PalmaGiuseppina FrascaBeatrice AiachiniSonia CremascoliAntonino MassoneClaudia OggerinoMaria Pia OnestaLucia RapisardaMaria Cristina PagliacciSauro BiscottoMichele ScarazzatoTiziana GiovanniniMimosa BalloniMarco CandelaPatrizia BrigidiCarlotte KiekensNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
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Medicine R Science Q Gabriele Bazzocchi Silvia Turroni Maria Chiara Bulzamini Federica D’Amico Angelica Bava Mirco Castiglioni Valentina Cagnetta Ernesto Losavio Maurizio Cazzaniga Laura Terenghi Luisa De Palma Giuseppina Frasca Beatrice Aiachini Sonia Cremascoli Antonino Massone Claudia Oggerino Maria Pia Onesta Lucia Rapisarda Maria Cristina Pagliacci Sauro Biscotto Michele Scarazzato Tiziana Giovannini Mimosa Balloni Marco Candela Patrizia Brigidi Carlotte Kiekens Changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion |
| description |
Abstract After spinal cord injury (SCI), patients face many physical and psychological issues including intestinal dysfunction and comorbidities, strongly affecting quality of life. The gut microbiota has recently been suggested to influence the course of the disease in these patients. However, to date only two studies have profiled the gut microbiota in SCI patients, months after a traumatic injury. Here we characterized the gut microbiota in a large Italian SCI population, within a short time from a not only traumatic injury. Feces were collected within the first week at the rehabilitation center (no later than 60 days after SCI), and profiled by 16S rRNA gene-based next-generation sequencing. Microbial profiles were compared to those publicly available of healthy age- and gender-matched Italians, and correlated to patient metadata, including type of SCI, spinal unit location, nutrition and concomitant antibiotic therapies. The gut microbiota of SCI patients shows distinct dysbiotic signatures, i.e. increase in potentially pathogenic, pro-inflammatory and mucus-degrading bacteria, and depletion of short-chain fatty acid producers. While robust to most host variables, such dysbiosis varies by lesion level and completeness, with the most neurologically impaired patients showing an even more unbalanced microbial profile. The SCI-related gut microbiome dysbiosis is very likely secondary to injury and closely related to the degree of completeness and severity of the lesion, regardless of etiology and time interval. This microbial layout could variously contribute to increased gut permeability and inflammation, potentially predisposing patients to the onset of severe comorbidities. |
| format |
article |
| author |
Gabriele Bazzocchi Silvia Turroni Maria Chiara Bulzamini Federica D’Amico Angelica Bava Mirco Castiglioni Valentina Cagnetta Ernesto Losavio Maurizio Cazzaniga Laura Terenghi Luisa De Palma Giuseppina Frasca Beatrice Aiachini Sonia Cremascoli Antonino Massone Claudia Oggerino Maria Pia Onesta Lucia Rapisarda Maria Cristina Pagliacci Sauro Biscotto Michele Scarazzato Tiziana Giovannini Mimosa Balloni Marco Candela Patrizia Brigidi Carlotte Kiekens |
| author_facet |
Gabriele Bazzocchi Silvia Turroni Maria Chiara Bulzamini Federica D’Amico Angelica Bava Mirco Castiglioni Valentina Cagnetta Ernesto Losavio Maurizio Cazzaniga Laura Terenghi Luisa De Palma Giuseppina Frasca Beatrice Aiachini Sonia Cremascoli Antonino Massone Claudia Oggerino Maria Pia Onesta Lucia Rapisarda Maria Cristina Pagliacci Sauro Biscotto Michele Scarazzato Tiziana Giovannini Mimosa Balloni Marco Candela Patrizia Brigidi Carlotte Kiekens |
| author_sort |
Gabriele Bazzocchi |
| title |
Changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion |
| title_short |
Changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion |
| title_full |
Changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion |
| title_fullStr |
Changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion |
| title_full_unstemmed |
Changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion |
| title_sort |
changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/9bb5424d0eb64cb2876bbe61a7628595 |
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