Changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion

Abstract After spinal cord injury (SCI), patients face many physical and psychological issues including intestinal dysfunction and comorbidities, strongly affecting quality of life. The gut microbiota has recently been suggested to influence the course of the disease in these patients. However, to d...

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Autores principales: Gabriele Bazzocchi, Silvia Turroni, Maria Chiara Bulzamini, Federica D’Amico, Angelica Bava, Mirco Castiglioni, Valentina Cagnetta, Ernesto Losavio, Maurizio Cazzaniga, Laura Terenghi, Luisa De Palma, Giuseppina Frasca, Beatrice Aiachini, Sonia Cremascoli, Antonino Massone, Claudia Oggerino, Maria Pia Onesta, Lucia Rapisarda, Maria Cristina Pagliacci, Sauro Biscotto, Michele Scarazzato, Tiziana Giovannini, Mimosa Balloni, Marco Candela, Patrizia Brigidi, Carlotte Kiekens
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Publicado: Nature Portfolio 2021
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spelling oai:doaj.org-article:9bb5424d0eb64cb2876bbe61a76285952021-12-02T16:04:13ZChanges in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion10.1038/s41598-021-92027-z2045-2322https://doaj.org/article/9bb5424d0eb64cb2876bbe61a76285952021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92027-zhttps://doaj.org/toc/2045-2322Abstract After spinal cord injury (SCI), patients face many physical and psychological issues including intestinal dysfunction and comorbidities, strongly affecting quality of life. The gut microbiota has recently been suggested to influence the course of the disease in these patients. However, to date only two studies have profiled the gut microbiota in SCI patients, months after a traumatic injury. Here we characterized the gut microbiota in a large Italian SCI population, within a short time from a not only traumatic injury. Feces were collected within the first week at the rehabilitation center (no later than 60 days after SCI), and profiled by 16S rRNA gene-based next-generation sequencing. Microbial profiles were compared to those publicly available of healthy age- and gender-matched Italians, and correlated to patient metadata, including type of SCI, spinal unit location, nutrition and concomitant antibiotic therapies. The gut microbiota of SCI patients shows distinct dysbiotic signatures, i.e. increase in potentially pathogenic, pro-inflammatory and mucus-degrading bacteria, and depletion of short-chain fatty acid producers. While robust to most host variables, such dysbiosis varies by lesion level and completeness, with the most neurologically impaired patients showing an even more unbalanced microbial profile. The SCI-related gut microbiome dysbiosis is very likely secondary to injury and closely related to the degree of completeness and severity of the lesion, regardless of etiology and time interval. This microbial layout could variously contribute to increased gut permeability and inflammation, potentially predisposing patients to the onset of severe comorbidities.Gabriele BazzocchiSilvia TurroniMaria Chiara BulzaminiFederica D’AmicoAngelica BavaMirco CastiglioniValentina CagnettaErnesto LosavioMaurizio CazzanigaLaura TerenghiLuisa De PalmaGiuseppina FrascaBeatrice AiachiniSonia CremascoliAntonino MassoneClaudia OggerinoMaria Pia OnestaLucia RapisardaMaria Cristina PagliacciSauro BiscottoMichele ScarazzatoTiziana GiovanniniMimosa BalloniMarco CandelaPatrizia BrigidiCarlotte KiekensNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Gabriele Bazzocchi
Silvia Turroni
Maria Chiara Bulzamini
Federica D’Amico
Angelica Bava
Mirco Castiglioni
Valentina Cagnetta
Ernesto Losavio
Maurizio Cazzaniga
Laura Terenghi
Luisa De Palma
Giuseppina Frasca
Beatrice Aiachini
Sonia Cremascoli
Antonino Massone
Claudia Oggerino
Maria Pia Onesta
Lucia Rapisarda
Maria Cristina Pagliacci
Sauro Biscotto
Michele Scarazzato
Tiziana Giovannini
Mimosa Balloni
Marco Candela
Patrizia Brigidi
Carlotte Kiekens
Changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion
description Abstract After spinal cord injury (SCI), patients face many physical and psychological issues including intestinal dysfunction and comorbidities, strongly affecting quality of life. The gut microbiota has recently been suggested to influence the course of the disease in these patients. However, to date only two studies have profiled the gut microbiota in SCI patients, months after a traumatic injury. Here we characterized the gut microbiota in a large Italian SCI population, within a short time from a not only traumatic injury. Feces were collected within the first week at the rehabilitation center (no later than 60 days after SCI), and profiled by 16S rRNA gene-based next-generation sequencing. Microbial profiles were compared to those publicly available of healthy age- and gender-matched Italians, and correlated to patient metadata, including type of SCI, spinal unit location, nutrition and concomitant antibiotic therapies. The gut microbiota of SCI patients shows distinct dysbiotic signatures, i.e. increase in potentially pathogenic, pro-inflammatory and mucus-degrading bacteria, and depletion of short-chain fatty acid producers. While robust to most host variables, such dysbiosis varies by lesion level and completeness, with the most neurologically impaired patients showing an even more unbalanced microbial profile. The SCI-related gut microbiome dysbiosis is very likely secondary to injury and closely related to the degree of completeness and severity of the lesion, regardless of etiology and time interval. This microbial layout could variously contribute to increased gut permeability and inflammation, potentially predisposing patients to the onset of severe comorbidities.
format article
author Gabriele Bazzocchi
Silvia Turroni
Maria Chiara Bulzamini
Federica D’Amico
Angelica Bava
Mirco Castiglioni
Valentina Cagnetta
Ernesto Losavio
Maurizio Cazzaniga
Laura Terenghi
Luisa De Palma
Giuseppina Frasca
Beatrice Aiachini
Sonia Cremascoli
Antonino Massone
Claudia Oggerino
Maria Pia Onesta
Lucia Rapisarda
Maria Cristina Pagliacci
Sauro Biscotto
Michele Scarazzato
Tiziana Giovannini
Mimosa Balloni
Marco Candela
Patrizia Brigidi
Carlotte Kiekens
author_facet Gabriele Bazzocchi
Silvia Turroni
Maria Chiara Bulzamini
Federica D’Amico
Angelica Bava
Mirco Castiglioni
Valentina Cagnetta
Ernesto Losavio
Maurizio Cazzaniga
Laura Terenghi
Luisa De Palma
Giuseppina Frasca
Beatrice Aiachini
Sonia Cremascoli
Antonino Massone
Claudia Oggerino
Maria Pia Onesta
Lucia Rapisarda
Maria Cristina Pagliacci
Sauro Biscotto
Michele Scarazzato
Tiziana Giovannini
Mimosa Balloni
Marco Candela
Patrizia Brigidi
Carlotte Kiekens
author_sort Gabriele Bazzocchi
title Changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion
title_short Changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion
title_full Changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion
title_fullStr Changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion
title_full_unstemmed Changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion
title_sort changes in gut microbiota in the acute phase after spinal cord injury correlate with severity of the lesion
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/9bb5424d0eb64cb2876bbe61a7628595
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