Mouse transcobalamin has features resembling both human transcobalamin and haptocorrin.

In humans, the cobalamin (Cbl) -binding protein transcobalamin (TC) transports Cbl from the intestine and into all the cells of the body, whereas the glycoprotein haptocorrin (HC), which is present in both blood and exocrine secretions, is able to bind also corrinoids other than Cbl. The aim of this...

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Autores principales:	Katrine Hygum, Dorte L Lildballe, Eva H Greibe, Anne L Morkbak, Steen S Poulsen, Boe S Sorensen, Torben E Petersen, Ebba Nexo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9bbaddec7bbf48a2a4f4052f0a8a0582
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spelling	oai:doaj.org-article:9bbaddec7bbf48a2a4f4052f0a8a05822021-11-18T06:52:56ZMouse transcobalamin has features resembling both human transcobalamin and haptocorrin.1932-620310.1371/journal.pone.0020638https://doaj.org/article/9bbaddec7bbf48a2a4f4052f0a8a05822011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21655200/?tool=EBIhttps://doaj.org/toc/1932-6203In humans, the cobalamin (Cbl) -binding protein transcobalamin (TC) transports Cbl from the intestine and into all the cells of the body, whereas the glycoprotein haptocorrin (HC), which is present in both blood and exocrine secretions, is able to bind also corrinoids other than Cbl. The aim of this study is to explore the expression of the Cbl-binding protein HC as well as TC in mice. BLAST analysis showed no homologous gene coding for HC in mice. Submaxillary glands and serum displayed one protein capable of binding Cbl. This Cbl-binding protein was purified from 300 submaxillary glands by affinity chromatography. Subsequent sequencing identified the protein as TC. Further characterization in terms of glycosylation status and binding specificity to the Cbl-analogue cobinamide revealed that mouse TC does not bind Concanavalin A sepharose (like human TC), but is capable of binding cobinamide (like human HC). Antibodies raised against mouse TC identified the protein in secretory cells of the submaxillary gland and in the ducts of the mammary gland, i.e. at locations where HC is also found in humans. Analysis of the TC-mRNA level showed a high TC transcript level in these glands and also in the kidney. By precipitation to insolubilised antibodies against mouse TC, we also showed that >97% of the Cbl-binding capacity and >98% of the Cbl were precipitated in serum. This indicates that TC is the only Cbl-binding protein in the mouse circulation. Our data show that TC but not HC is present in the mouse. Mouse TC is observed in tissues where humans express TC and/or HC. Mouse TC has features in common with both human TC and HC. Our results suggest that the Cbl-binding proteins present in the circulation and exocrine glands may vary amongst species.Katrine HygumDorte L LildballeEva H GreibeAnne L MorkbakSteen S PoulsenBoe S SorensenTorben E PetersenEbba NexoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e20638 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katrine Hygum Dorte L Lildballe Eva H Greibe Anne L Morkbak Steen S Poulsen Boe S Sorensen Torben E Petersen Ebba Nexo Mouse transcobalamin has features resembling both human transcobalamin and haptocorrin.
description	In humans, the cobalamin (Cbl) -binding protein transcobalamin (TC) transports Cbl from the intestine and into all the cells of the body, whereas the glycoprotein haptocorrin (HC), which is present in both blood and exocrine secretions, is able to bind also corrinoids other than Cbl. The aim of this study is to explore the expression of the Cbl-binding protein HC as well as TC in mice. BLAST analysis showed no homologous gene coding for HC in mice. Submaxillary glands and serum displayed one protein capable of binding Cbl. This Cbl-binding protein was purified from 300 submaxillary glands by affinity chromatography. Subsequent sequencing identified the protein as TC. Further characterization in terms of glycosylation status and binding specificity to the Cbl-analogue cobinamide revealed that mouse TC does not bind Concanavalin A sepharose (like human TC), but is capable of binding cobinamide (like human HC). Antibodies raised against mouse TC identified the protein in secretory cells of the submaxillary gland and in the ducts of the mammary gland, i.e. at locations where HC is also found in humans. Analysis of the TC-mRNA level showed a high TC transcript level in these glands and also in the kidney. By precipitation to insolubilised antibodies against mouse TC, we also showed that >97% of the Cbl-binding capacity and >98% of the Cbl were precipitated in serum. This indicates that TC is the only Cbl-binding protein in the mouse circulation. Our data show that TC but not HC is present in the mouse. Mouse TC is observed in tissues where humans express TC and/or HC. Mouse TC has features in common with both human TC and HC. Our results suggest that the Cbl-binding proteins present in the circulation and exocrine glands may vary amongst species.
format	article
author	Katrine Hygum Dorte L Lildballe Eva H Greibe Anne L Morkbak Steen S Poulsen Boe S Sorensen Torben E Petersen Ebba Nexo
author_facet	Katrine Hygum Dorte L Lildballe Eva H Greibe Anne L Morkbak Steen S Poulsen Boe S Sorensen Torben E Petersen Ebba Nexo
author_sort	Katrine Hygum
title	Mouse transcobalamin has features resembling both human transcobalamin and haptocorrin.
title_short	Mouse transcobalamin has features resembling both human transcobalamin and haptocorrin.
title_full	Mouse transcobalamin has features resembling both human transcobalamin and haptocorrin.
title_fullStr	Mouse transcobalamin has features resembling both human transcobalamin and haptocorrin.
title_full_unstemmed	Mouse transcobalamin has features resembling both human transcobalamin and haptocorrin.
title_sort	mouse transcobalamin has features resembling both human transcobalamin and haptocorrin.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/9bbaddec7bbf48a2a4f4052f0a8a0582
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Mouse transcobalamin has features resembling both human transcobalamin and haptocorrin.

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