Rod and cone pathway signalling is altered in the P2X7 receptor knock out mouse.

The P2X7 receptor (P2X7-R) is expressed in the retina and brain and has been implicated in neurodegenerative diseases. However, whether it is expressed by neurons and plays a role as a neurotransmitter receptor has been the subject of controversy. In this study, we first show that the novel vesicula...

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Autores principales:	Kirstan A Vessey, Erica L Fletcher
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9bcacc4a0cef4ad2b0c00a94efff2f1c
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spelling	oai:doaj.org-article:9bcacc4a0cef4ad2b0c00a94efff2f1c2021-11-18T07:30:37ZRod and cone pathway signalling is altered in the P2X7 receptor knock out mouse.1932-620310.1371/journal.pone.0029990https://doaj.org/article/9bcacc4a0cef4ad2b0c00a94efff2f1c2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22253851/?tool=EBIhttps://doaj.org/toc/1932-6203The P2X7 receptor (P2X7-R) is expressed in the retina and brain and has been implicated in neurodegenerative diseases. However, whether it is expressed by neurons and plays a role as a neurotransmitter receptor has been the subject of controversy. In this study, we first show that the novel vesicular transporter for ATP, VNUT, is expressed in the retina, verifying the presence of the molecular machinery for ATP to act as neurotransmitter at P2X7-Rs. Secondly we show the presence of P2X7-R mRNA and protein in the retina and cortex and absence of the full length variant 1 of the receptor in the P2X7-R knock out (P2X7-KO) mouse. The role of the P2X7-R in neuronal function of the retina was assessed by comparing the electroretinogram response of P2X7-KO with WT mice. The rod photoreceptor response was found to be similar, while both rod and cone pathway post-photoreceptor responses were significantly larger in P2X7-KO mice. This suggests that activation of P2X7-Rs modulates output of second order retinal neurons. In line with this finding, P2X7-Rs were found in the outer plexiform layer and on inner retinal cell classes, including horizontal, amacrine and ganglion cells. The receptor co-localized with conventional synapses in the IPL and was expressed on amacrine cells post-synaptic to rod bipolar ribbon synapses. In view of the changes in visual function in the P2X7-KO mouse and the immunocytochemical location of the receptor in the normal retina, it is likely the P2X7-R provides excitatory input to photoreceptor terminals or to inhibitory cells that shape both the rod and cone pathway response.Kirstan A VesseyErica L FletcherPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e29990 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kirstan A Vessey Erica L Fletcher Rod and cone pathway signalling is altered in the P2X7 receptor knock out mouse.
description	The P2X7 receptor (P2X7-R) is expressed in the retina and brain and has been implicated in neurodegenerative diseases. However, whether it is expressed by neurons and plays a role as a neurotransmitter receptor has been the subject of controversy. In this study, we first show that the novel vesicular transporter for ATP, VNUT, is expressed in the retina, verifying the presence of the molecular machinery for ATP to act as neurotransmitter at P2X7-Rs. Secondly we show the presence of P2X7-R mRNA and protein in the retina and cortex and absence of the full length variant 1 of the receptor in the P2X7-R knock out (P2X7-KO) mouse. The role of the P2X7-R in neuronal function of the retina was assessed by comparing the electroretinogram response of P2X7-KO with WT mice. The rod photoreceptor response was found to be similar, while both rod and cone pathway post-photoreceptor responses were significantly larger in P2X7-KO mice. This suggests that activation of P2X7-Rs modulates output of second order retinal neurons. In line with this finding, P2X7-Rs were found in the outer plexiform layer and on inner retinal cell classes, including horizontal, amacrine and ganglion cells. The receptor co-localized with conventional synapses in the IPL and was expressed on amacrine cells post-synaptic to rod bipolar ribbon synapses. In view of the changes in visual function in the P2X7-KO mouse and the immunocytochemical location of the receptor in the normal retina, it is likely the P2X7-R provides excitatory input to photoreceptor terminals or to inhibitory cells that shape both the rod and cone pathway response.
format	article
author	Kirstan A Vessey Erica L Fletcher
author_facet	Kirstan A Vessey Erica L Fletcher
author_sort	Kirstan A Vessey
title	Rod and cone pathway signalling is altered in the P2X7 receptor knock out mouse.
title_short	Rod and cone pathway signalling is altered in the P2X7 receptor knock out mouse.
title_full	Rod and cone pathway signalling is altered in the P2X7 receptor knock out mouse.
title_fullStr	Rod and cone pathway signalling is altered in the P2X7 receptor knock out mouse.
title_full_unstemmed	Rod and cone pathway signalling is altered in the P2X7 receptor knock out mouse.
title_sort	rod and cone pathway signalling is altered in the p2x7 receptor knock out mouse.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/9bcacc4a0cef4ad2b0c00a94efff2f1c
work_keys_str_mv	AT kirstanavessey rodandconepathwaysignallingisalteredinthep2x7receptorknockoutmouse AT ericalfletcher rodandconepathwaysignallingisalteredinthep2x7receptorknockoutmouse
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Rod and cone pathway signalling is altered in the P2X7 receptor knock out mouse.

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