Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia

Abstract. Ansharieta R, Ramandinianto SC, Effendi MH, Plumeriastuti H. 2021. Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia. Biodiversitas 22: 1600-1605. The emergence...

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Autores principales:	RIBBY ANSHARIETA, SANCAKA CHASYER RAMANDINIANTO, Mustofa Helmi Effendi, HANI PLUMERIASTUTI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	escherichia coli esbl blactx-m gene blatem gene raw cow’s milk human health Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9bdc41efeab54e50ad4577cf18603a95
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spelling	oai:doaj.org-article:9bdc41efeab54e50ad4577cf18603a952021-11-22T00:57:38ZMolecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia1412-033X2085-472210.13057/biodiv/d220402https://doaj.org/article/9bdc41efeab54e50ad4577cf18603a952021-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7329https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Ansharieta R, Ramandinianto SC, Effendi MH, Plumeriastuti H. 2021. Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia. Biodiversitas 22: 1600-1605. The emergence of extended-spectrum ?-lactamase (ESBL) producing bacteria and its increasing level has become public health issue. The presence of these bacteria in food of animal origin is quite alarming. The objective of this study was to detect and characterize Escherichia coli producing ESBL encoding genes, isolated from 200 raw cow milk samples in East Java, Indonesia. The results of this study showed that 70.5% of isolates were confirmed as E. coli, based on the morphological growth of colonies on the EMB Agar and biochemical IMViC tests. In this study, the double-disc synergy test (DDST) method was used to confirm the ESBL, and previously sorted out presumptively by using Aztreonam antibiotic disc. The antibiotics used were amoxicillin-clavulanate, ceftazidime, and cefotaxime for DDST. In addition, ESBL confirmation with Multiplex PCR method for blaCTX-M and blaTEM genes were done. The presence of ESBL-producing by E. coli isolated from raw cow’s milk in East Java were 2.12% (3/141). The PCR results showed that the double blaCTX-M and blaTEM gene harbored by 2 ESBL isolates and one blaTEM gene as many as 1 ESBL isolate. Thus, the findings of our study indicate that milk can be a good reservoir of bacteria carrying blaCTX-M and blaTEM ESBL resistance genes with the potential to affect human health.RIBBY ANSHARIETASANCAKA CHASYER RAMANDINIANTOMustofa Helmi EffendiHANI PLUMERIASTUTIMBI & UNS Soloarticleescherichia coliesblblactx-m geneblatem generaw cow’s milkhuman healthBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	escherichia coli esbl blactx-m gene blatem gene raw cow’s milk human health Biology (General) QH301-705.5
spellingShingle	escherichia coli esbl blactx-m gene blatem gene raw cow’s milk human health Biology (General) QH301-705.5 RIBBY ANSHARIETA SANCAKA CHASYER RAMANDINIANTO Mustofa Helmi Effendi HANI PLUMERIASTUTI Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia
description	Abstract. Ansharieta R, Ramandinianto SC, Effendi MH, Plumeriastuti H. 2021. Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia. Biodiversitas 22: 1600-1605. The emergence of extended-spectrum ?-lactamase (ESBL) producing bacteria and its increasing level has become public health issue. The presence of these bacteria in food of animal origin is quite alarming. The objective of this study was to detect and characterize Escherichia coli producing ESBL encoding genes, isolated from 200 raw cow milk samples in East Java, Indonesia. The results of this study showed that 70.5% of isolates were confirmed as E. coli, based on the morphological growth of colonies on the EMB Agar and biochemical IMViC tests. In this study, the double-disc synergy test (DDST) method was used to confirm the ESBL, and previously sorted out presumptively by using Aztreonam antibiotic disc. The antibiotics used were amoxicillin-clavulanate, ceftazidime, and cefotaxime for DDST. In addition, ESBL confirmation with Multiplex PCR method for blaCTX-M and blaTEM genes were done. The presence of ESBL-producing by E. coli isolated from raw cow’s milk in East Java were 2.12% (3/141). The PCR results showed that the double blaCTX-M and blaTEM gene harbored by 2 ESBL isolates and one blaTEM gene as many as 1 ESBL isolate. Thus, the findings of our study indicate that milk can be a good reservoir of bacteria carrying blaCTX-M and blaTEM ESBL resistance genes with the potential to affect human health.
format	article
author	RIBBY ANSHARIETA SANCAKA CHASYER RAMANDINIANTO Mustofa Helmi Effendi HANI PLUMERIASTUTI
author_facet	RIBBY ANSHARIETA SANCAKA CHASYER RAMANDINIANTO Mustofa Helmi Effendi HANI PLUMERIASTUTI
author_sort	RIBBY ANSHARIETA
title	Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia
title_short	Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia
title_full	Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia
title_fullStr	Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia
title_full_unstemmed	Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia
title_sort	molecular identification of blactx-m and blatem genes encoding extended-spectrum ß-lactamase (esbl) producing escherichia coli isolated from raw cow’s milk in east java, indonesia
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/9bdc41efeab54e50ad4577cf18603a95
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Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia

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