Immunomodulatory properties of graphene oxide for osteogenesis and angiogenesis

Deting Xue,1,* Erman Chen,1,* Huiming Zhong,2,* Wei Zhang,1 Shengdong Wang,1 Muhammad Umar Joomun,1 Tianyi Yao,3 Yanbin Tan,1 ShiSheng Lin,3 Qiang Zheng,1 Zhijun Pan1 1Department of Orthopaedics, The Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou 3...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xue D, Chen E, Zhong H, Zhang W, Wang S, Joomun MU, Yao T, Tan Y, Lin S, Zheng Q, Pan Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Graphene oxide osteo-immunomodulatory bone marrow mesenchymal stem cells osteogenesis angiogenesis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9be2aed91a1e499696871e077f772393
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9be2aed91a1e499696871e077f772393
record_format	dspace
spelling	oai:doaj.org-article:9be2aed91a1e499696871e077f7723932021-12-02T07:24:26ZImmunomodulatory properties of graphene oxide for osteogenesis and angiogenesis1178-2013https://doaj.org/article/9be2aed91a1e499696871e077f7723932018-09-01T00:00:00Zhttps://www.dovepress.com/immunomodulatory-properties-of-graphene-oxide-for-osteogenesis-and-ang-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Deting Xue,1,* Erman Chen,1,* Huiming Zhong,2,* Wei Zhang,1 Shengdong Wang,1 Muhammad Umar Joomun,1 Tianyi Yao,3 Yanbin Tan,1 ShiSheng Lin,3 Qiang Zheng,1 Zhijun Pan1 1Department of Orthopaedics, The Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou 310009, People’s Republic of China; 2Department of Emergency, The Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou 310009, People’s Republic of China; 3Department of Information Science and Electronic Engineering and State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, People’s Republic of China *These authors contributed equally to this work Background: The osteo-immunomodulatory properties of biomaterials play an important role in the outcomes of bone regeneration. Graphene oxide (GO) has been widely applied in many research fields due to its unique properties. However, the immunomodulatory properties of GO as a biomaterial for bone tissue engineering are still unclear. Materials and methods: In this study, we evaluated the Inflammatory response of RAW264.7 cells influenced by GO. Then the osteogenic differentiation of BMSCs, and angiogenic differentiation of human umbilical vein endothelial cells (HUVECs) by stimulation with GO/RAW 264.7-conditioned culture medium were accessed. We also further investigated the possible mechanisms underlying the osteo- and angio-immunomodulatory effects of GO. Results: Our results showed that GO stimulates the secretion of oncostatin M, tumor necrosis factor alpha and other factors through the nuclear factor-κB pathway. GO/RAW264.7-conditioned medium promoted the osteogenic differentiation of BMSCs, stimulated upregulation of the HUVECs of vascular-related receptors, and promoted their tube formation in vitro. Conclusion: In conclusion, our research shows that GO, as a biomaterial, can induce the formation of a beneficial osteo-immunomodulatory environment and is a promising biomaterial for bone tissue engineering. Keywords: graphene oxide, osteo-immunomodulatory, bone marrow mesenchymal stem cells, osteogenesis, angiogenesis, RAW264.7 cells, HUVECsXue DChen EZhong HZhang WWang SJoomun MUYao TTan YLin SZheng QPan ZDove Medical PressarticleGraphene oxideosteo-immunomodulatorybone marrow mesenchymal stem cellsosteogenesisangiogenesisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 5799-5810 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Graphene oxide osteo-immunomodulatory bone marrow mesenchymal stem cells osteogenesis angiogenesis Medicine (General) R5-920
spellingShingle	Graphene oxide osteo-immunomodulatory bone marrow mesenchymal stem cells osteogenesis angiogenesis Medicine (General) R5-920 Xue D Chen E Zhong H Zhang W Wang S Joomun MU Yao T Tan Y Lin S Zheng Q Pan Z Immunomodulatory properties of graphene oxide for osteogenesis and angiogenesis
description	Deting Xue,1,* Erman Chen,1,* Huiming Zhong,2,* Wei Zhang,1 Shengdong Wang,1 Muhammad Umar Joomun,1 Tianyi Yao,3 Yanbin Tan,1 ShiSheng Lin,3 Qiang Zheng,1 Zhijun Pan1 1Department of Orthopaedics, The Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou 310009, People’s Republic of China; 2Department of Emergency, The Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou 310009, People’s Republic of China; 3Department of Information Science and Electronic Engineering and State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, People’s Republic of China *These authors contributed equally to this work Background: The osteo-immunomodulatory properties of biomaterials play an important role in the outcomes of bone regeneration. Graphene oxide (GO) has been widely applied in many research fields due to its unique properties. However, the immunomodulatory properties of GO as a biomaterial for bone tissue engineering are still unclear. Materials and methods: In this study, we evaluated the Inflammatory response of RAW264.7 cells influenced by GO. Then the osteogenic differentiation of BMSCs, and angiogenic differentiation of human umbilical vein endothelial cells (HUVECs) by stimulation with GO/RAW 264.7-conditioned culture medium were accessed. We also further investigated the possible mechanisms underlying the osteo- and angio-immunomodulatory effects of GO. Results: Our results showed that GO stimulates the secretion of oncostatin M, tumor necrosis factor alpha and other factors through the nuclear factor-κB pathway. GO/RAW264.7-conditioned medium promoted the osteogenic differentiation of BMSCs, stimulated upregulation of the HUVECs of vascular-related receptors, and promoted their tube formation in vitro. Conclusion: In conclusion, our research shows that GO, as a biomaterial, can induce the formation of a beneficial osteo-immunomodulatory environment and is a promising biomaterial for bone tissue engineering. Keywords: graphene oxide, osteo-immunomodulatory, bone marrow mesenchymal stem cells, osteogenesis, angiogenesis, RAW264.7 cells, HUVECs
format	article
author	Xue D Chen E Zhong H Zhang W Wang S Joomun MU Yao T Tan Y Lin S Zheng Q Pan Z
author_facet	Xue D Chen E Zhong H Zhang W Wang S Joomun MU Yao T Tan Y Lin S Zheng Q Pan Z
author_sort	Xue D
title	Immunomodulatory properties of graphene oxide for osteogenesis and angiogenesis
title_short	Immunomodulatory properties of graphene oxide for osteogenesis and angiogenesis
title_full	Immunomodulatory properties of graphene oxide for osteogenesis and angiogenesis
title_fullStr	Immunomodulatory properties of graphene oxide for osteogenesis and angiogenesis
title_full_unstemmed	Immunomodulatory properties of graphene oxide for osteogenesis and angiogenesis
title_sort	immunomodulatory properties of graphene oxide for osteogenesis and angiogenesis
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/9be2aed91a1e499696871e077f772393
work_keys_str_mv	AT xued immunomodulatorypropertiesofgrapheneoxideforosteogenesisandangiogenesis AT chene immunomodulatorypropertiesofgrapheneoxideforosteogenesisandangiogenesis AT zhongh immunomodulatorypropertiesofgrapheneoxideforosteogenesisandangiogenesis AT zhangw immunomodulatorypropertiesofgrapheneoxideforosteogenesisandangiogenesis AT wangs immunomodulatorypropertiesofgrapheneoxideforosteogenesisandangiogenesis AT joomunmu immunomodulatorypropertiesofgrapheneoxideforosteogenesisandangiogenesis AT yaot immunomodulatorypropertiesofgrapheneoxideforosteogenesisandangiogenesis AT tany immunomodulatorypropertiesofgrapheneoxideforosteogenesisandangiogenesis AT lins immunomodulatorypropertiesofgrapheneoxideforosteogenesisandangiogenesis AT zhengq immunomodulatorypropertiesofgrapheneoxideforosteogenesisandangiogenesis AT panz immunomodulatorypropertiesofgrapheneoxideforosteogenesisandangiogenesis
_version_	1718399467354324992

Immunomodulatory properties of graphene oxide for osteogenesis and angiogenesis

Ejemplares similares