Toxicity evaluation of biodegradable chitosan nanoparticles using a zebrafish embryo model

Yu-Lan Hu1, Wang Qi1, Feng Han2, Jian-Zhong Shao3, Jian-Qing Gao11Institute of Pharmaceutics, College of Pharmaceutical Sciences, 2Institute of Pharmacology, Toxicology and Biochemical Pharmaceutics, 3College of Life Sciences, Key Laboratory for Cell and Gene Engineering of Zhejiang Province, Zhejia...

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Autores principales:	Hu YL, Qi W, Han F, Shao JZ, Gao JQ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9be59109ad0b431291afff4fd136d05f
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spelling	oai:doaj.org-article:9be59109ad0b431291afff4fd136d05f2021-12-02T03:01:53ZToxicity evaluation of biodegradable chitosan nanoparticles using a zebrafish embryo model1176-91141178-2013https://doaj.org/article/9be59109ad0b431291afff4fd136d05f2011-12-01T00:00:00Zhttp://www.dovepress.com/toxicity-evaluation-of-biodegradable-chitosan-nanoparticles-using-a-ze-a8872https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yu-Lan Hu1, Wang Qi1, Feng Han2, Jian-Zhong Shao3, Jian-Qing Gao11Institute of Pharmaceutics, College of Pharmaceutical Sciences, 2Institute of Pharmacology, Toxicology and Biochemical Pharmaceutics, 3College of Life Sciences, Key Laboratory for Cell and Gene Engineering of Zhejiang Province, Zhejiang University, Hangzhou, Zhejiang, People&#39;s Republic of ChinaBackground: Although there are a number of reports regarding the toxicity evaluation of inorganic nanoparticles, knowledge on biodegradable nanomaterials, which have always been considered safe, is still limited. For example, the toxicity of chitosan nanoparticles, one of the most widely used drug/gene delivery vehicles, is largely unknown. In the present study, the zebrafish model was used for a safety evaluation of this nanocarrier.Methods: Chitosan nanoparticles with two particle sizes were prepared by ionic cross-linking of chitosan with sodium tripolyphosphate. Chitosan nanoparticles of different concentrations were incubated with zebrafish embryos, and ZnO nanoparticles were used as the positive control.Results: Embryo exposure to chitosan nanoparticles and ZnO nanoparticles resulted in a decreased hatching rate and increased mortality, which was concentration-dependent. Chitosan nanoparticles at a size of 200 nm caused malformations, including a bent spine, pericardial edema, and an opaque yolk in zebrafish embryos. Furthermore, embryos exposed to chitosan nanoparticles showed an increased rate of cell death, high expression of reactive oxygen species, as well as overexpression of heat shock protein 70, indicating that chitosan nanoparticles can cause physiological stress in zebrafish. The results also suggest that the toxicity of biodegradable nanocarriers such as chitosan nanoparticles must be addressed, especially considering the in vivo distribution of these nanoscaled particles.Conclusion: Our results add new insights into the potential toxicity of nanoparticles produced by biodegradable materials, and may help us to understand better the nanotoxicity of drug delivery carriers.Keywords: chitosan, nanoparticles, zebrafish embryo, nanotoxicologyHu YLQi WHan FShao JZGao JQDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 3351-3359 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Hu YL Qi W Han F Shao JZ Gao JQ Toxicity evaluation of biodegradable chitosan nanoparticles using a zebrafish embryo model
description	Yu-Lan Hu1, Wang Qi1, Feng Han2, Jian-Zhong Shao3, Jian-Qing Gao11Institute of Pharmaceutics, College of Pharmaceutical Sciences, 2Institute of Pharmacology, Toxicology and Biochemical Pharmaceutics, 3College of Life Sciences, Key Laboratory for Cell and Gene Engineering of Zhejiang Province, Zhejiang University, Hangzhou, Zhejiang, People&#39;s Republic of ChinaBackground: Although there are a number of reports regarding the toxicity evaluation of inorganic nanoparticles, knowledge on biodegradable nanomaterials, which have always been considered safe, is still limited. For example, the toxicity of chitosan nanoparticles, one of the most widely used drug/gene delivery vehicles, is largely unknown. In the present study, the zebrafish model was used for a safety evaluation of this nanocarrier.Methods: Chitosan nanoparticles with two particle sizes were prepared by ionic cross-linking of chitosan with sodium tripolyphosphate. Chitosan nanoparticles of different concentrations were incubated with zebrafish embryos, and ZnO nanoparticles were used as the positive control.Results: Embryo exposure to chitosan nanoparticles and ZnO nanoparticles resulted in a decreased hatching rate and increased mortality, which was concentration-dependent. Chitosan nanoparticles at a size of 200 nm caused malformations, including a bent spine, pericardial edema, and an opaque yolk in zebrafish embryos. Furthermore, embryos exposed to chitosan nanoparticles showed an increased rate of cell death, high expression of reactive oxygen species, as well as overexpression of heat shock protein 70, indicating that chitosan nanoparticles can cause physiological stress in zebrafish. The results also suggest that the toxicity of biodegradable nanocarriers such as chitosan nanoparticles must be addressed, especially considering the in vivo distribution of these nanoscaled particles.Conclusion: Our results add new insights into the potential toxicity of nanoparticles produced by biodegradable materials, and may help us to understand better the nanotoxicity of drug delivery carriers.Keywords: chitosan, nanoparticles, zebrafish embryo, nanotoxicology
format	article
author	Hu YL Qi W Han F Shao JZ Gao JQ
author_facet	Hu YL Qi W Han F Shao JZ Gao JQ
author_sort	Hu YL
title	Toxicity evaluation of biodegradable chitosan nanoparticles using a zebrafish embryo model
title_short	Toxicity evaluation of biodegradable chitosan nanoparticles using a zebrafish embryo model
title_full	Toxicity evaluation of biodegradable chitosan nanoparticles using a zebrafish embryo model
title_fullStr	Toxicity evaluation of biodegradable chitosan nanoparticles using a zebrafish embryo model
title_full_unstemmed	Toxicity evaluation of biodegradable chitosan nanoparticles using a zebrafish embryo model
title_sort	toxicity evaluation of biodegradable chitosan nanoparticles using a zebrafish embryo model
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/9be59109ad0b431291afff4fd136d05f
work_keys_str_mv	AT huyl toxicityevaluationofbiodegradablechitosannanoparticlesusingazebrafishembryomodel AT qiw toxicityevaluationofbiodegradablechitosannanoparticlesusingazebrafishembryomodel AT hanf toxicityevaluationofbiodegradablechitosannanoparticlesusingazebrafishembryomodel AT shaojz toxicityevaluationofbiodegradablechitosannanoparticlesusingazebrafishembryomodel AT gaojq toxicityevaluationofbiodegradablechitosannanoparticlesusingazebrafishembryomodel
_version_	1718401997647904768

Toxicity evaluation of biodegradable chitosan nanoparticles using a zebrafish embryo model

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