Characterizing short and long term iron stress responses in iron deficiency tolerant and susceptible soybean (Glycine max L. Merr.)

Nutrient deficiencies limit growth and yield in many different crop species. The calcareous soils in the upper Midwestern United States favor the development of iron deficiency chlorosis (IDC) in soybean (Glycine max L. Merr.). Even minor symptoms result in end of season yield loss. To identify mole...

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Autores principales:	Leorrie Atencio, Justin Salazar, Adrienne N. Moran Lauter, Michael D. Gonzales, Jamie A. O'Rourke, Michelle A. Graham
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Glycine max Iron deficiency Iron stress Abiotic stress Transcriptomics Plant ecology QK900-989
Acceso en línea:	https://doaj.org/article/9be61b3e6f4d4b3583afef3368720105
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spelling	oai:doaj.org-article:9be61b3e6f4d4b3583afef33687201052021-12-04T04:36:31ZCharacterizing short and long term iron stress responses in iron deficiency tolerant and susceptible soybean (Glycine max L. Merr.)2667-064X10.1016/j.stress.2021.100012https://doaj.org/article/9be61b3e6f4d4b3583afef33687201052021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667064X21000117https://doaj.org/toc/2667-064XNutrient deficiencies limit growth and yield in many different crop species. The calcareous soils in the upper Midwestern United States favor the development of iron deficiency chlorosis (IDC) in soybean (Glycine max L. Merr.). Even minor symptoms result in end of season yield loss. To identify molecular pathways and networks underlying tolerance to iron deficiency stress in soybean, we leveraged two near isogenic lines (Clark and Isoclark) that differ in their tolerance to iron stress. Clark and Isoclark were grown in hydroponics in one of three treatments: iron sufficient media for ten days, iron deficient media for ten days, or iron sufficient media for eight days followed by transfer to iron deficient media for two days. Following phenotyping, plant tissues were harvested for RNA-seq analysis. To facilitate analyses, we clustered differentially expressed genes across genotypes and timepoints in both roots and leaves. With this experimental design, we could identify iron stress response differences between Clark and Isoclark, evaluate the impact of iron stress duration, and link expression clusters with discrete biological functions and the transcription factor families that regulate them. We identified thousands of differentially expressed genes associated with soybean stress tolerant responses including the cell cycle, gene silencing, iron acquisition, and defense. In Clark, the number of differentially expressed genes and magnitude of expression increased with increasing iron stress duration. In contrast, Isoclark decreased the number and magnitude of differentially expressed genes across time. Differences in gene expression corresponded to phenotypic differences. The shifting expression patterns between timepoints and tissues suggests novel mechanisms for iron stress signaling between source and sink tissues.Leorrie AtencioJustin SalazarAdrienne N. Moran LauterMichael D. GonzalesJamie A. O'RourkeMichelle A. GrahamElsevierarticleGlycine maxIron deficiencyIron stressAbiotic stressTranscriptomicsPlant ecologyQK900-989ENPlant Stress, Vol 2, Iss , Pp 100012- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Glycine max Iron deficiency Iron stress Abiotic stress Transcriptomics Plant ecology QK900-989
spellingShingle	Glycine max Iron deficiency Iron stress Abiotic stress Transcriptomics Plant ecology QK900-989 Leorrie Atencio Justin Salazar Adrienne N. Moran Lauter Michael D. Gonzales Jamie A. O'Rourke Michelle A. Graham Characterizing short and long term iron stress responses in iron deficiency tolerant and susceptible soybean (Glycine max L. Merr.)
description	Nutrient deficiencies limit growth and yield in many different crop species. The calcareous soils in the upper Midwestern United States favor the development of iron deficiency chlorosis (IDC) in soybean (Glycine max L. Merr.). Even minor symptoms result in end of season yield loss. To identify molecular pathways and networks underlying tolerance to iron deficiency stress in soybean, we leveraged two near isogenic lines (Clark and Isoclark) that differ in their tolerance to iron stress. Clark and Isoclark were grown in hydroponics in one of three treatments: iron sufficient media for ten days, iron deficient media for ten days, or iron sufficient media for eight days followed by transfer to iron deficient media for two days. Following phenotyping, plant tissues were harvested for RNA-seq analysis. To facilitate analyses, we clustered differentially expressed genes across genotypes and timepoints in both roots and leaves. With this experimental design, we could identify iron stress response differences between Clark and Isoclark, evaluate the impact of iron stress duration, and link expression clusters with discrete biological functions and the transcription factor families that regulate them. We identified thousands of differentially expressed genes associated with soybean stress tolerant responses including the cell cycle, gene silencing, iron acquisition, and defense. In Clark, the number of differentially expressed genes and magnitude of expression increased with increasing iron stress duration. In contrast, Isoclark decreased the number and magnitude of differentially expressed genes across time. Differences in gene expression corresponded to phenotypic differences. The shifting expression patterns between timepoints and tissues suggests novel mechanisms for iron stress signaling between source and sink tissues.
format	article
author	Leorrie Atencio Justin Salazar Adrienne N. Moran Lauter Michael D. Gonzales Jamie A. O'Rourke Michelle A. Graham
author_facet	Leorrie Atencio Justin Salazar Adrienne N. Moran Lauter Michael D. Gonzales Jamie A. O'Rourke Michelle A. Graham
author_sort	Leorrie Atencio
title	Characterizing short and long term iron stress responses in iron deficiency tolerant and susceptible soybean (Glycine max L. Merr.)
title_short	Characterizing short and long term iron stress responses in iron deficiency tolerant and susceptible soybean (Glycine max L. Merr.)
title_full	Characterizing short and long term iron stress responses in iron deficiency tolerant and susceptible soybean (Glycine max L. Merr.)
title_fullStr	Characterizing short and long term iron stress responses in iron deficiency tolerant and susceptible soybean (Glycine max L. Merr.)
title_full_unstemmed	Characterizing short and long term iron stress responses in iron deficiency tolerant and susceptible soybean (Glycine max L. Merr.)
title_sort	characterizing short and long term iron stress responses in iron deficiency tolerant and susceptible soybean (glycine max l. merr.)
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/9be61b3e6f4d4b3583afef3368720105
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Characterizing short and long term iron stress responses in iron deficiency tolerant and susceptible soybean (Glycine max L. Merr.)

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