Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling

Abstract Particulate autologous tooth roots are increasingly used for alveolar bone augmentation; however, the proteomic profile of acid dentin lysate and the respective cellular response have not been investigated. Here we show that TGF-β1 is among the 226 proteins of acid dentin lysate (ADL) prepa...

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Autores principales:	Jila Nasirzade, Zahra Kargarpour, Goran Mitulović, Franz Josef Strauss, Layla Panahipour, Frank Schwarz, Reinhard Gruber
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9be7b8c26a6840f7b771ad19afe7eaa9
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spelling	oai:doaj.org-article:9be7b8c26a6840f7b771ad19afe7eaa92021-12-02T17:52:41ZProteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling10.1038/s41598-021-89996-62045-2322https://doaj.org/article/9be7b8c26a6840f7b771ad19afe7eaa92021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89996-6https://doaj.org/toc/2045-2322Abstract Particulate autologous tooth roots are increasingly used for alveolar bone augmentation; however, the proteomic profile of acid dentin lysate and the respective cellular response have not been investigated. Here we show that TGF-β1 is among the 226 proteins of acid dentin lysate (ADL) prepared from porcine teeth. RNA sequencing identified 231 strongly regulated genes when gingival fibroblasts were exposed to ADL. Out of these genes, about one third required activation of the TGF-β receptor type I kinase including interleukin 11 (IL11) and NADPH oxidase 4 (NOX4). Reverse transcription-quantitative polymerase chain reaction and immunoassay confirmed the TGF-β-dependent expression of IL11 and NOX4. The activation of canonical TGF-β signaling by ADL was further confirmed by the phosphorylation of Smad3 and translocation of Smad2/3, using Western blot and immunofluorescence staining, respectively. Finally, we showed that TGF-β activity released from dentin by acid lysis adsorbs to titanium and collagen membranes. These findings suggest that dentin particles are a rich source of TGF-β causing a major response of gingival fibroblasts.Jila NasirzadeZahra KargarpourGoran MitulovićFranz Josef StraussLayla PanahipourFrank SchwarzReinhard GruberNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jila Nasirzade Zahra Kargarpour Goran Mitulović Franz Josef Strauss Layla Panahipour Frank Schwarz Reinhard Gruber Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling
description	Abstract Particulate autologous tooth roots are increasingly used for alveolar bone augmentation; however, the proteomic profile of acid dentin lysate and the respective cellular response have not been investigated. Here we show that TGF-β1 is among the 226 proteins of acid dentin lysate (ADL) prepared from porcine teeth. RNA sequencing identified 231 strongly regulated genes when gingival fibroblasts were exposed to ADL. Out of these genes, about one third required activation of the TGF-β receptor type I kinase including interleukin 11 (IL11) and NADPH oxidase 4 (NOX4). Reverse transcription-quantitative polymerase chain reaction and immunoassay confirmed the TGF-β-dependent expression of IL11 and NOX4. The activation of canonical TGF-β signaling by ADL was further confirmed by the phosphorylation of Smad3 and translocation of Smad2/3, using Western blot and immunofluorescence staining, respectively. Finally, we showed that TGF-β activity released from dentin by acid lysis adsorbs to titanium and collagen membranes. These findings suggest that dentin particles are a rich source of TGF-β causing a major response of gingival fibroblasts.
format	article
author	Jila Nasirzade Zahra Kargarpour Goran Mitulović Franz Josef Strauss Layla Panahipour Frank Schwarz Reinhard Gruber
author_facet	Jila Nasirzade Zahra Kargarpour Goran Mitulović Franz Josef Strauss Layla Panahipour Frank Schwarz Reinhard Gruber
author_sort	Jila Nasirzade
title	Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling
title_short	Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling
title_full	Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling
title_fullStr	Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling
title_full_unstemmed	Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling
title_sort	proteomic and genomic analysis of acid dentin lysate with focus on tgf-β signaling
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9be7b8c26a6840f7b771ad19afe7eaa9
work_keys_str_mv	AT jilanasirzade proteomicandgenomicanalysisofaciddentinlysatewithfocusontgfbsignaling AT zahrakargarpour proteomicandgenomicanalysisofaciddentinlysatewithfocusontgfbsignaling AT goranmitulovic proteomicandgenomicanalysisofaciddentinlysatewithfocusontgfbsignaling AT franzjosefstrauss proteomicandgenomicanalysisofaciddentinlysatewithfocusontgfbsignaling AT laylapanahipour proteomicandgenomicanalysisofaciddentinlysatewithfocusontgfbsignaling AT frankschwarz proteomicandgenomicanalysisofaciddentinlysatewithfocusontgfbsignaling AT reinhardgruber proteomicandgenomicanalysisofaciddentinlysatewithfocusontgfbsignaling
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Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling

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