Doxorubicin Delivered Using Nanoparticles Camouflaged with Mesenchymal Stem Cell Membranes to Treat Colon Cancer

Yi Liu,1 Jingtong Zhao,2 Jinlan Jiang,2 Fangfang Chen,1,3,4 Xuedong Fang1,3 1Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, People’s Republic of China; 2Department of Central Laboratory, China-Japan Union H...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Liu Y, Zhao J, Jiang J, Chen F, Fang X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	iron oxide mesenchymal stem cells doxorubicin colon cancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9beacafac403497f9b82f2087ae172e5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9beacafac403497f9b82f2087ae172e5
record_format	dspace
spelling	oai:doaj.org-article:9beacafac403497f9b82f2087ae172e52021-12-02T08:49:43ZDoxorubicin Delivered Using Nanoparticles Camouflaged with Mesenchymal Stem Cell Membranes to Treat Colon Cancer1178-2013https://doaj.org/article/9beacafac403497f9b82f2087ae172e52020-04-01T00:00:00Zhttps://www.dovepress.com/doxorubicin-delivered-using-nanoparticles-camouflaged-with-mesenchymal-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yi Liu,1 Jingtong Zhao,2 Jinlan Jiang,2 Fangfang Chen,1,3,4 Xuedong Fang1,3 1Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, People’s Republic of China; 2Department of Central Laboratory, China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, People’s Republic of China; 3State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun, Jilin 130012, People’s Republic of China; 4Key Laboratory of Zoonoses Research, Ministry of Education, Jilin University, Changchun, Jilin 130062, People’s Republic of ChinaCorrespondence: Xuedong Fang; Fangfang Chen Email fangxd1961@163.com; cffemail@163.comPurpose: The primary goal of the present study was to design doxorubicin (DOX)-loaded superparamagnetic iron oxide (SPIO) nanoparticles (NPs) coated with mesenchymal stem cell (MSC) membranes and explore their effect on colon cancer in vitro and in vivo.Methods: DOX-SPIO NPs were coated with MSC membranes using an extruder, and the morphological characteristics of MSC membrane-camouflaged nanodrug (DOX-SPIO@MSCs) evaluated by transmission electron microscopy (TEM) and NP-tracking analysis. Drug loading and pH response were assessed by UV spectrophotometry. Intracellular colocalization was analyzed using NP-treated MC38 cells stained with 3,3′-dioctadecyloxacarbocyanine perchlorate and Hoechst 33342. Cellular uptake was analyzed using an inverted fluorescence microscope and flow cytometry and cytotoxicity evaluated by cell counting kit-8 assay. Biological compatibility was assessed by hemolysis analysis, immunoactivation test and leukocyte uptake experiments. Furthermore, intravenous injection of chemotherapy drugs into MC38 tumor-bearing C57BL/6 mice was used to study anti-tumor effects.Results: Typical core-shell NP structures were observed by TEM. Particle size remained stable in fetal bovine serum and phosphate-buffered saline (PBS). Compared with DOX-SPIO, DOX-SPIO@MSCs improved cellular uptake efficiency, enhanced anti-tumor effects, and reduced the immune system response. Animal experiments demonstrated that DOX-SPIO@MSCs enhanced tumor treatment efficacy while reducing systemic side effects.Conclusion: Our experimental results demonstrate that DOX-SPIO@MSCs are a promising targeted nanocarrier for application in treatment of colon cancer.Keywords: iron oxide, mesenchymal stem cells, doxorubicin, colon cancerLiu YZhao JJiang JChen FFang XDove Medical Pressarticleiron oxidemesenchymal stem cellsdoxorubicincolon cancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 2873-2884 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	iron oxide mesenchymal stem cells doxorubicin colon cancer Medicine (General) R5-920
spellingShingle	iron oxide mesenchymal stem cells doxorubicin colon cancer Medicine (General) R5-920 Liu Y Zhao J Jiang J Chen F Fang X Doxorubicin Delivered Using Nanoparticles Camouflaged with Mesenchymal Stem Cell Membranes to Treat Colon Cancer
description	Yi Liu,1 Jingtong Zhao,2 Jinlan Jiang,2 Fangfang Chen,1,3,4 Xuedong Fang1,3 1Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, People’s Republic of China; 2Department of Central Laboratory, China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, People’s Republic of China; 3State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun, Jilin 130012, People’s Republic of China; 4Key Laboratory of Zoonoses Research, Ministry of Education, Jilin University, Changchun, Jilin 130062, People’s Republic of ChinaCorrespondence: Xuedong Fang; Fangfang Chen Email fangxd1961@163.com; cffemail@163.comPurpose: The primary goal of the present study was to design doxorubicin (DOX)-loaded superparamagnetic iron oxide (SPIO) nanoparticles (NPs) coated with mesenchymal stem cell (MSC) membranes and explore their effect on colon cancer in vitro and in vivo.Methods: DOX-SPIO NPs were coated with MSC membranes using an extruder, and the morphological characteristics of MSC membrane-camouflaged nanodrug (DOX-SPIO@MSCs) evaluated by transmission electron microscopy (TEM) and NP-tracking analysis. Drug loading and pH response were assessed by UV spectrophotometry. Intracellular colocalization was analyzed using NP-treated MC38 cells stained with 3,3′-dioctadecyloxacarbocyanine perchlorate and Hoechst 33342. Cellular uptake was analyzed using an inverted fluorescence microscope and flow cytometry and cytotoxicity evaluated by cell counting kit-8 assay. Biological compatibility was assessed by hemolysis analysis, immunoactivation test and leukocyte uptake experiments. Furthermore, intravenous injection of chemotherapy drugs into MC38 tumor-bearing C57BL/6 mice was used to study anti-tumor effects.Results: Typical core-shell NP structures were observed by TEM. Particle size remained stable in fetal bovine serum and phosphate-buffered saline (PBS). Compared with DOX-SPIO, DOX-SPIO@MSCs improved cellular uptake efficiency, enhanced anti-tumor effects, and reduced the immune system response. Animal experiments demonstrated that DOX-SPIO@MSCs enhanced tumor treatment efficacy while reducing systemic side effects.Conclusion: Our experimental results demonstrate that DOX-SPIO@MSCs are a promising targeted nanocarrier for application in treatment of colon cancer.Keywords: iron oxide, mesenchymal stem cells, doxorubicin, colon cancer
format	article
author	Liu Y Zhao J Jiang J Chen F Fang X
author_facet	Liu Y Zhao J Jiang J Chen F Fang X
author_sort	Liu Y
title	Doxorubicin Delivered Using Nanoparticles Camouflaged with Mesenchymal Stem Cell Membranes to Treat Colon Cancer
title_short	Doxorubicin Delivered Using Nanoparticles Camouflaged with Mesenchymal Stem Cell Membranes to Treat Colon Cancer
title_full	Doxorubicin Delivered Using Nanoparticles Camouflaged with Mesenchymal Stem Cell Membranes to Treat Colon Cancer
title_fullStr	Doxorubicin Delivered Using Nanoparticles Camouflaged with Mesenchymal Stem Cell Membranes to Treat Colon Cancer
title_full_unstemmed	Doxorubicin Delivered Using Nanoparticles Camouflaged with Mesenchymal Stem Cell Membranes to Treat Colon Cancer
title_sort	doxorubicin delivered using nanoparticles camouflaged with mesenchymal stem cell membranes to treat colon cancer
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/9beacafac403497f9b82f2087ae172e5
work_keys_str_mv	AT liuy doxorubicindeliveredusingnanoparticlescamouflagedwithmesenchymalstemcellmembranestotreatcoloncancer AT zhaoj doxorubicindeliveredusingnanoparticlescamouflagedwithmesenchymalstemcellmembranestotreatcoloncancer AT jiangj doxorubicindeliveredusingnanoparticlescamouflagedwithmesenchymalstemcellmembranestotreatcoloncancer AT chenf doxorubicindeliveredusingnanoparticlescamouflagedwithmesenchymalstemcellmembranestotreatcoloncancer AT fangx doxorubicindeliveredusingnanoparticlescamouflagedwithmesenchymalstemcellmembranestotreatcoloncancer
_version_	1718398379734597632

Doxorubicin Delivered Using Nanoparticles Camouflaged with Mesenchymal Stem Cell Membranes to Treat Colon Cancer

Ejemplares similares