Functional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.

Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV) associated malignancy with high prevalence in Southern Chinese. In order to assess whether defects of EBV-specific immunity may contribute to the tumor, the phenotype and function of circulating T-cells and tumor infiltrating lymphocytes...

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Autores principales:	Jiang Li, Xue-hui Zeng, Hao-yuan Mo, Ulrika Rolén, Yan-fang Gao, Xiao-shi Zhang, Qiu-yan Chen, Li Zhang, Mu-sheng Zeng, Man-zhi Li, Wen-lin Huang, Xiao-ning Wang, Yi-xin Zeng, Maria G Masucci
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9bf255423df54bef952ea29681975c60
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spelling	oai:doaj.org-article:9bf255423df54bef952ea29681975c602021-11-25T06:10:33ZFunctional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.1932-620310.1371/journal.pone.0001122https://doaj.org/article/9bf255423df54bef952ea29681975c602007-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0001122https://doaj.org/toc/1932-6203Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV) associated malignancy with high prevalence in Southern Chinese. In order to assess whether defects of EBV-specific immunity may contribute to the tumor, the phenotype and function of circulating T-cells and tumor infiltrating lymphocytes (TILs) were investigated in untreated NPC patients. Circulating naïve CD3+CD45RA+ and CD4+CD25- cells were decreased, while activated CD4+CD25+ T-cells and CD3-CD16+ NK-cells were increased in patients compared to healthy donors. The frequency of T-cells recognizing seven HLA-A2 restricted epitopes in LMP1 and LMP2 was lower in the patients and remained low after stimulation with autologous EBV-carrying cells. TILs expanded in low doses of IL-2 exhibited an increase of CD3+CD4+, CD3+CD45RO+ and CD4+CD25+ cells and 2 to 5 fold higher frequency of LMP1 and LMP2 tetramer positive cells compared to peripheral blood. EBV-specific cytotoxicity could be reactivated from the blood of most patients, whereas the TILs lacked cytotoxic activity and failed to produce IFNgamma upon specific stimulation. Thus, EBV-specific rejection responses appear to be functionally inactivated at the tumor site in NPC.Jiang LiXue-hui ZengHao-yuan MoUlrika RolénYan-fang GaoXiao-shi ZhangQiu-yan ChenLi ZhangMu-sheng ZengMan-zhi LiWen-lin HuangXiao-ning WangYi-xin ZengMaria G MasucciPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 2, Iss 11, p e1122 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiang Li Xue-hui Zeng Hao-yuan Mo Ulrika Rolén Yan-fang Gao Xiao-shi Zhang Qiu-yan Chen Li Zhang Mu-sheng Zeng Man-zhi Li Wen-lin Huang Xiao-ning Wang Yi-xin Zeng Maria G Masucci Functional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.
description	Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV) associated malignancy with high prevalence in Southern Chinese. In order to assess whether defects of EBV-specific immunity may contribute to the tumor, the phenotype and function of circulating T-cells and tumor infiltrating lymphocytes (TILs) were investigated in untreated NPC patients. Circulating naïve CD3+CD45RA+ and CD4+CD25- cells were decreased, while activated CD4+CD25+ T-cells and CD3-CD16+ NK-cells were increased in patients compared to healthy donors. The frequency of T-cells recognizing seven HLA-A2 restricted epitopes in LMP1 and LMP2 was lower in the patients and remained low after stimulation with autologous EBV-carrying cells. TILs expanded in low doses of IL-2 exhibited an increase of CD3+CD4+, CD3+CD45RO+ and CD4+CD25+ cells and 2 to 5 fold higher frequency of LMP1 and LMP2 tetramer positive cells compared to peripheral blood. EBV-specific cytotoxicity could be reactivated from the blood of most patients, whereas the TILs lacked cytotoxic activity and failed to produce IFNgamma upon specific stimulation. Thus, EBV-specific rejection responses appear to be functionally inactivated at the tumor site in NPC.
format	article
author	Jiang Li Xue-hui Zeng Hao-yuan Mo Ulrika Rolén Yan-fang Gao Xiao-shi Zhang Qiu-yan Chen Li Zhang Mu-sheng Zeng Man-zhi Li Wen-lin Huang Xiao-ning Wang Yi-xin Zeng Maria G Masucci
author_facet	Jiang Li Xue-hui Zeng Hao-yuan Mo Ulrika Rolén Yan-fang Gao Xiao-shi Zhang Qiu-yan Chen Li Zhang Mu-sheng Zeng Man-zhi Li Wen-lin Huang Xiao-ning Wang Yi-xin Zeng Maria G Masucci
author_sort	Jiang Li
title	Functional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.
title_short	Functional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.
title_full	Functional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.
title_fullStr	Functional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.
title_full_unstemmed	Functional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.
title_sort	functional inactivation of ebv-specific t-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/9bf255423df54bef952ea29681975c60
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Functional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.

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