Effect of sequence depth and length in long-read assembly of the maize inbred NC358

Sequence depth and read length determine the quality of genome assembly. Here, the authors leverage a set of PacBio reads to develop guidelines for sequencing and assembly of complex plant genomes in order to allocate finite resources using maize as an example.

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Autores principales:	Shujun Ou, Jianing Liu, Kapeel M. Chougule, Arkarachai Fungtammasan, Arun S. Seetharam, Joshua C. Stein, Victor Llaca, Nancy Manchanda, Amanda M. Gilbert, Sharon Wei, Chen-Shan Chin, David E. Hufnagel, Sarah Pedersen, Samantha J. Snodgrass, Kevin Fengler, Margaret Woodhouse, Brian P. Walenz, Sergey Koren, Adam M. Phillippy, Brett T. Hannigan, R. Kelly Dawe, Candice N. Hirsch, Matthew B. Hufford, Doreen Ware
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/9bfad55a4173428f8acffe7c51e13452
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spelling	oai:doaj.org-article:9bfad55a4173428f8acffe7c51e134522021-12-02T14:41:13ZEffect of sequence depth and length in long-read assembly of the maize inbred NC35810.1038/s41467-020-16037-72041-1723https://doaj.org/article/9bfad55a4173428f8acffe7c51e134522020-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16037-7https://doaj.org/toc/2041-1723Sequence depth and read length determine the quality of genome assembly. Here, the authors leverage a set of PacBio reads to develop guidelines for sequencing and assembly of complex plant genomes in order to allocate finite resources using maize as an example.Shujun OuJianing LiuKapeel M. ChouguleArkarachai FungtammasanArun S. SeetharamJoshua C. SteinVictor LlacaNancy ManchandaAmanda M. GilbertSharon WeiChen-Shan ChinDavid E. HufnagelSarah PedersenSamantha J. SnodgrassKevin FenglerMargaret WoodhouseBrian P. WalenzSergey KorenAdam M. PhillippyBrett T. HanniganR. Kelly DaweCandice N. HirschMatthew B. HuffordDoreen WareNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Shujun Ou Jianing Liu Kapeel M. Chougule Arkarachai Fungtammasan Arun S. Seetharam Joshua C. Stein Victor Llaca Nancy Manchanda Amanda M. Gilbert Sharon Wei Chen-Shan Chin David E. Hufnagel Sarah Pedersen Samantha J. Snodgrass Kevin Fengler Margaret Woodhouse Brian P. Walenz Sergey Koren Adam M. Phillippy Brett T. Hannigan R. Kelly Dawe Candice N. Hirsch Matthew B. Hufford Doreen Ware Effect of sequence depth and length in long-read assembly of the maize inbred NC358
description	Sequence depth and read length determine the quality of genome assembly. Here, the authors leverage a set of PacBio reads to develop guidelines for sequencing and assembly of complex plant genomes in order to allocate finite resources using maize as an example.
format	article
author	Shujun Ou Jianing Liu Kapeel M. Chougule Arkarachai Fungtammasan Arun S. Seetharam Joshua C. Stein Victor Llaca Nancy Manchanda Amanda M. Gilbert Sharon Wei Chen-Shan Chin David E. Hufnagel Sarah Pedersen Samantha J. Snodgrass Kevin Fengler Margaret Woodhouse Brian P. Walenz Sergey Koren Adam M. Phillippy Brett T. Hannigan R. Kelly Dawe Candice N. Hirsch Matthew B. Hufford Doreen Ware
author_facet	Shujun Ou Jianing Liu Kapeel M. Chougule Arkarachai Fungtammasan Arun S. Seetharam Joshua C. Stein Victor Llaca Nancy Manchanda Amanda M. Gilbert Sharon Wei Chen-Shan Chin David E. Hufnagel Sarah Pedersen Samantha J. Snodgrass Kevin Fengler Margaret Woodhouse Brian P. Walenz Sergey Koren Adam M. Phillippy Brett T. Hannigan R. Kelly Dawe Candice N. Hirsch Matthew B. Hufford Doreen Ware
author_sort	Shujun Ou
title	Effect of sequence depth and length in long-read assembly of the maize inbred NC358
title_short	Effect of sequence depth and length in long-read assembly of the maize inbred NC358
title_full	Effect of sequence depth and length in long-read assembly of the maize inbred NC358
title_fullStr	Effect of sequence depth and length in long-read assembly of the maize inbred NC358
title_full_unstemmed	Effect of sequence depth and length in long-read assembly of the maize inbred NC358
title_sort	effect of sequence depth and length in long-read assembly of the maize inbred nc358
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/9bfad55a4173428f8acffe7c51e13452
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Effect of sequence depth and length in long-read assembly of the maize inbred NC358

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