3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats

Abstract Magnetic Resonance Imaging (MRI) appears as a good surrogate to Computed Tomography (CT) scan as it does not involve radiation. In this context, a 3D anatomical and perfusion MR imaging protocol was developed to follow the evolution of bone regeneration and the neo-vascularization in femora...

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Autores principales:	Emeline J. Ribot, Clement Tournier, Rachida Aid-Launais, Neha Koonjoo, Hugo Oliveira, Aurelien J. Trotier, Sylvie Rey, Didier Wecker, Didier Letourneur, Joelle Amedee Vilamitjana, Sylvain Miraux
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9bfca30bace742c2841b3d24dbbc9523
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spelling	oai:doaj.org-article:9bfca30bace742c2841b3d24dbbc95232021-12-02T15:06:09Z3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats10.1038/s41598-017-06258-02045-2322https://doaj.org/article/9bfca30bace742c2841b3d24dbbc95232017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06258-0https://doaj.org/toc/2045-2322Abstract Magnetic Resonance Imaging (MRI) appears as a good surrogate to Computed Tomography (CT) scan as it does not involve radiation. In this context, a 3D anatomical and perfusion MR imaging protocol was developed to follow the evolution of bone regeneration and the neo-vascularization in femoral bone defects in rats. For this, three different biomaterials based on Pullulan-Dextran and containing either Fucoidan or HydroxyApatite or both were implanted. In vivo MRI, ex vivo micro-CT and histology were performed 1, 3 and 5 weeks after implantation. The high spatially resolved (156 × 182 × 195 µm) anatomical images showed a high contrast from the defects filled with biomaterials that decreased over time due to bone formation. The 3D Dynamic Contrast Enhanced (DCE) imaging with high temporal resolution (1 image/19 s) enabled to detect a modification in the Area-Under-The-Gadolinium-Curve over the weeks post implantation. The high sensitivity of MRI enabled to distinguish which biomaterial was the least efficient for bone regeneration, which was confirmed by micro-CT images and by a lower vessel density observed by histology. In conclusion, the methodology developed here highlights the efficiency of longitudinal MRI for tissue engineering as a routine small animal exam.Emeline J. RibotClement TournierRachida Aid-LaunaisNeha KoonjooHugo OliveiraAurelien J. TrotierSylvie ReyDidier WeckerDidier LetourneurJoelle Amedee VilamitjanaSylvain MirauxNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emeline J. Ribot Clement Tournier Rachida Aid-Launais Neha Koonjoo Hugo Oliveira Aurelien J. Trotier Sylvie Rey Didier Wecker Didier Letourneur Joelle Amedee Vilamitjana Sylvain Miraux 3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats
description	Abstract Magnetic Resonance Imaging (MRI) appears as a good surrogate to Computed Tomography (CT) scan as it does not involve radiation. In this context, a 3D anatomical and perfusion MR imaging protocol was developed to follow the evolution of bone regeneration and the neo-vascularization in femoral bone defects in rats. For this, three different biomaterials based on Pullulan-Dextran and containing either Fucoidan or HydroxyApatite or both were implanted. In vivo MRI, ex vivo micro-CT and histology were performed 1, 3 and 5 weeks after implantation. The high spatially resolved (156 × 182 × 195 µm) anatomical images showed a high contrast from the defects filled with biomaterials that decreased over time due to bone formation. The 3D Dynamic Contrast Enhanced (DCE) imaging with high temporal resolution (1 image/19 s) enabled to detect a modification in the Area-Under-The-Gadolinium-Curve over the weeks post implantation. The high sensitivity of MRI enabled to distinguish which biomaterial was the least efficient for bone regeneration, which was confirmed by micro-CT images and by a lower vessel density observed by histology. In conclusion, the methodology developed here highlights the efficiency of longitudinal MRI for tissue engineering as a routine small animal exam.
format	article
author	Emeline J. Ribot Clement Tournier Rachida Aid-Launais Neha Koonjoo Hugo Oliveira Aurelien J. Trotier Sylvie Rey Didier Wecker Didier Letourneur Joelle Amedee Vilamitjana Sylvain Miraux
author_facet	Emeline J. Ribot Clement Tournier Rachida Aid-Launais Neha Koonjoo Hugo Oliveira Aurelien J. Trotier Sylvie Rey Didier Wecker Didier Letourneur Joelle Amedee Vilamitjana Sylvain Miraux
author_sort	Emeline J. Ribot
title	3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats
title_short	3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats
title_full	3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats
title_fullStr	3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats
title_full_unstemmed	3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats
title_sort	3d anatomical and perfusion mri for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9bfca30bace742c2841b3d24dbbc9523
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3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats

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