10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees

Abstract In bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the environmental component (food), a genetic component is also involved in caste differentiation. This mechanism has not yet been fu...

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Autores principales:	Luiza Diniz Ferreira Borges, Letícia Leandro Batista, Serena Mares Malta, Tamiris Sabrina Rodrigues, Jéssica Regina da Costa Silva, Gabriela Venturini, Alexandre da Costa Pereira, Pedro Henrique Gonçalves Guedes, Carlos Ueira-Vieira, Ana Maria Bonetti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9bfdd03ce58844a2829a686d6bd39021
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spelling	oai:doaj.org-article:9bfdd03ce58844a2829a686d6bd390212021-12-02T16:57:37Z10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees10.1038/s41598-021-89212-52045-2322https://doaj.org/article/9bfdd03ce58844a2829a686d6bd390212021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89212-5https://doaj.org/toc/2045-2322Abstract In bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the environmental component (food), a genetic component is also involved in caste differentiation. This mechanism has not yet been fully elucidated and may involve epigenetic and metabolic regulation. Here, we verified that the genes encoding histone deacetylases HDAC1 and HDAC4 and histone acetyltransferase KAT2A were expressed at all stages of Melipona scutellaris, with fluctuations between developmental stages and castes. In larvae, the HDAC genes showed the same profile of Juvenile Hormone titers—previous reported—whereas the HAT gene exhibited the opposite profile. We also investigated the larvae and larval food metabolomes, but we did not identify the putative queen-fate inducing compounds, geraniol and 10-hydroxy-2E-decenoic acid (10HDA). Finally, we demonstrated that the histone deacetylase inhibitor 10HDA—the major lipid component of royal jelly and hence a putative regulator of honeybee caste differentiation—was unable to promote differentiation in queens in Melipona scutellaris. Our results suggest that epigenetic and hormonal regulations may act synergistically to drive caste differentiation in Melipona and that 10HDA is not a caste-differentiation factor in Melipona scutellaris.Luiza Diniz Ferreira BorgesLetícia Leandro BatistaSerena Mares MaltaTamiris Sabrina RodriguesJéssica Regina da Costa SilvaGabriela VenturiniAlexandre da Costa PereiraPedro Henrique Gonçalves GuedesCarlos Ueira-VieiraAna Maria BonettiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luiza Diniz Ferreira Borges Letícia Leandro Batista Serena Mares Malta Tamiris Sabrina Rodrigues Jéssica Regina da Costa Silva Gabriela Venturini Alexandre da Costa Pereira Pedro Henrique Gonçalves Guedes Carlos Ueira-Vieira Ana Maria Bonetti 10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees
description	Abstract In bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the environmental component (food), a genetic component is also involved in caste differentiation. This mechanism has not yet been fully elucidated and may involve epigenetic and metabolic regulation. Here, we verified that the genes encoding histone deacetylases HDAC1 and HDAC4 and histone acetyltransferase KAT2A were expressed at all stages of Melipona scutellaris, with fluctuations between developmental stages and castes. In larvae, the HDAC genes showed the same profile of Juvenile Hormone titers—previous reported—whereas the HAT gene exhibited the opposite profile. We also investigated the larvae and larval food metabolomes, but we did not identify the putative queen-fate inducing compounds, geraniol and 10-hydroxy-2E-decenoic acid (10HDA). Finally, we demonstrated that the histone deacetylase inhibitor 10HDA—the major lipid component of royal jelly and hence a putative regulator of honeybee caste differentiation—was unable to promote differentiation in queens in Melipona scutellaris. Our results suggest that epigenetic and hormonal regulations may act synergistically to drive caste differentiation in Melipona and that 10HDA is not a caste-differentiation factor in Melipona scutellaris.
format	article
author	Luiza Diniz Ferreira Borges Letícia Leandro Batista Serena Mares Malta Tamiris Sabrina Rodrigues Jéssica Regina da Costa Silva Gabriela Venturini Alexandre da Costa Pereira Pedro Henrique Gonçalves Guedes Carlos Ueira-Vieira Ana Maria Bonetti
author_facet	Luiza Diniz Ferreira Borges Letícia Leandro Batista Serena Mares Malta Tamiris Sabrina Rodrigues Jéssica Regina da Costa Silva Gabriela Venturini Alexandre da Costa Pereira Pedro Henrique Gonçalves Guedes Carlos Ueira-Vieira Ana Maria Bonetti
author_sort	Luiza Diniz Ferreira Borges
title	10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees
title_short	10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees
title_full	10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees
title_fullStr	10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees
title_full_unstemmed	10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees
title_sort	10-hydroxy-2e-decenoic acid (10hda) does not promote caste differentiation in melipona scutellaris stingless bees
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9bfdd03ce58844a2829a686d6bd39021
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10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees

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