Multi-beam two-photon polymerization for fast large area 3D periodic structure fabrication for bioapplications

Abstract Two-photon polymerization (TPP) is capable of fabricating 3D structures with dimensions from sub-µm to a few hundred µm. As a direct laser writing (DLW) process, fabrication time of 3D TPP structures scale with the third order, limiting its use in large volume fabrication. Here, we report o...

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Autores principales:	Christian Maibohm, Oscar F. Silvestre, Jérôme Borme, Maina Sinou, Kevin Heggarty, Jana B. Nieder
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9c04f57cd2ab4995b9acca6f03a246a6
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spelling	oai:doaj.org-article:9c04f57cd2ab4995b9acca6f03a246a62021-12-02T14:47:31ZMulti-beam two-photon polymerization for fast large area 3D periodic structure fabrication for bioapplications10.1038/s41598-020-64955-92045-2322https://doaj.org/article/9c04f57cd2ab4995b9acca6f03a246a62020-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-64955-9https://doaj.org/toc/2045-2322Abstract Two-photon polymerization (TPP) is capable of fabricating 3D structures with dimensions from sub-µm to a few hundred µm. As a direct laser writing (DLW) process, fabrication time of 3D TPP structures scale with the third order, limiting its use in large volume fabrication. Here, we report on a scalable fabrication method that cuts fabrication time to a fraction. A parallelized 9 multi-beamlets DLW process, created by a fixed diffraction optical element (DOE) and subsequent stitching are used to fabricate large periodic high aspect ratio 3D microstructured arrays with sub-micron features spanning several hundred of µm2. The wall structure in the array is designed with a minimum of traced lines and is created by a low numerical aperture (NA) microscope objective, leading to self-supporting lines omitting the need for line-hatching. The fabricated periodic arrays are applied in a cell – 3D microstructure interaction study using living HeLa cells. First indications of increased cell proliferation in the presence of 3D microstructures compared to planar surfaces are obtained. Furthermore, the cells adopt an elongated morphology when attached to the 3D microstructured surfaces. Both results constitute promising findings rendering the 3D microstructures a suited tool for cell interaction experiments, e.g. for cell migration, separation or even tissue engineering studies.Christian MaibohmOscar F. SilvestreJérôme BormeMaina SinouKevin HeggartyJana B. NiederNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian Maibohm Oscar F. Silvestre Jérôme Borme Maina Sinou Kevin Heggarty Jana B. Nieder Multi-beam two-photon polymerization for fast large area 3D periodic structure fabrication for bioapplications
description	Abstract Two-photon polymerization (TPP) is capable of fabricating 3D structures with dimensions from sub-µm to a few hundred µm. As a direct laser writing (DLW) process, fabrication time of 3D TPP structures scale with the third order, limiting its use in large volume fabrication. Here, we report on a scalable fabrication method that cuts fabrication time to a fraction. A parallelized 9 multi-beamlets DLW process, created by a fixed diffraction optical element (DOE) and subsequent stitching are used to fabricate large periodic high aspect ratio 3D microstructured arrays with sub-micron features spanning several hundred of µm2. The wall structure in the array is designed with a minimum of traced lines and is created by a low numerical aperture (NA) microscope objective, leading to self-supporting lines omitting the need for line-hatching. The fabricated periodic arrays are applied in a cell – 3D microstructure interaction study using living HeLa cells. First indications of increased cell proliferation in the presence of 3D microstructures compared to planar surfaces are obtained. Furthermore, the cells adopt an elongated morphology when attached to the 3D microstructured surfaces. Both results constitute promising findings rendering the 3D microstructures a suited tool for cell interaction experiments, e.g. for cell migration, separation or even tissue engineering studies.
format	article
author	Christian Maibohm Oscar F. Silvestre Jérôme Borme Maina Sinou Kevin Heggarty Jana B. Nieder
author_facet	Christian Maibohm Oscar F. Silvestre Jérôme Borme Maina Sinou Kevin Heggarty Jana B. Nieder
author_sort	Christian Maibohm
title	Multi-beam two-photon polymerization for fast large area 3D periodic structure fabrication for bioapplications
title_short	Multi-beam two-photon polymerization for fast large area 3D periodic structure fabrication for bioapplications
title_full	Multi-beam two-photon polymerization for fast large area 3D periodic structure fabrication for bioapplications
title_fullStr	Multi-beam two-photon polymerization for fast large area 3D periodic structure fabrication for bioapplications
title_full_unstemmed	Multi-beam two-photon polymerization for fast large area 3D periodic structure fabrication for bioapplications
title_sort	multi-beam two-photon polymerization for fast large area 3d periodic structure fabrication for bioapplications
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/9c04f57cd2ab4995b9acca6f03a246a6
work_keys_str_mv	AT christianmaibohm multibeamtwophotonpolymerizationforfastlargearea3dperiodicstructurefabricationforbioapplications AT oscarfsilvestre multibeamtwophotonpolymerizationforfastlargearea3dperiodicstructurefabricationforbioapplications AT jeromeborme multibeamtwophotonpolymerizationforfastlargearea3dperiodicstructurefabricationforbioapplications AT mainasinou multibeamtwophotonpolymerizationforfastlargearea3dperiodicstructurefabricationforbioapplications AT kevinheggarty multibeamtwophotonpolymerizationforfastlargearea3dperiodicstructurefabricationforbioapplications AT janabnieder multibeamtwophotonpolymerizationforfastlargearea3dperiodicstructurefabricationforbioapplications
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Multi-beam two-photon polymerization for fast large area 3D periodic structure fabrication for bioapplications

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