Contribution of the cyclic nucleotide gated channel subunit, CNG-3, to olfactory plasticity in Caenorhabditis elegans

Abstract In Caenorhabditis elegans, the AWC neurons are thought to deploy a cGMP signaling cascade in the detection of and response to AWC sensed odors. Prolonged exposure to an AWC sensed odor in the absence of food leads to reversible decreases in the animal’s attraction to that odor. This adaptat...

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Autores principales:	Damien M. O’Halloran, Svetlana Altshuler-Keylin, Xiao-Dong Zhang, Chao He, Christopher Morales-Phan, Yawei Yu, Julia A. Kaye, Chantal Brueggemann, Tsung-Yu Chen, Noelle D. L’Etoile
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9c0987815bae4206933dea42b37c80bd
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spelling	oai:doaj.org-article:9c0987815bae4206933dea42b37c80bd2021-12-02T16:06:05ZContribution of the cyclic nucleotide gated channel subunit, CNG-3, to olfactory plasticity in Caenorhabditis elegans10.1038/s41598-017-00126-72045-2322https://doaj.org/article/9c0987815bae4206933dea42b37c80bd2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00126-7https://doaj.org/toc/2045-2322Abstract In Caenorhabditis elegans, the AWC neurons are thought to deploy a cGMP signaling cascade in the detection of and response to AWC sensed odors. Prolonged exposure to an AWC sensed odor in the absence of food leads to reversible decreases in the animal’s attraction to that odor. This adaptation exhibits two stages referred to as short-term and long-term adaptation. Previously, the protein kinase G (PKG), EGL-4/PKG-1, was shown necessary for both stages of adaptation and phosphorylation of its target, the beta-type cyclic nucleotide gated (CNG) channel subunit, TAX-2, was implicated in the short term stage. Here we uncover a novel role for the CNG channel subunit, CNG-3, in short term adaptation. We demonstrate that CNG-3 is required in the AWC for adaptation to short (thirty minute) exposures of odor, and contains a candidate PKG phosphorylation site required to tune odor sensitivity. We also provide in vivo data suggesting that CNG-3 forms a complex with both TAX-2 and TAX-4 CNG channel subunits in AWC. Finally, we examine the physiology of different CNG channel subunit combinations.Damien M. O’HalloranSvetlana Altshuler-KeylinXiao-Dong ZhangChao HeChristopher Morales-PhanYawei YuJulia A. KayeChantal BrueggemannTsung-Yu ChenNoelle D. L’EtoileNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Damien M. O’Halloran Svetlana Altshuler-Keylin Xiao-Dong Zhang Chao He Christopher Morales-Phan Yawei Yu Julia A. Kaye Chantal Brueggemann Tsung-Yu Chen Noelle D. L’Etoile Contribution of the cyclic nucleotide gated channel subunit, CNG-3, to olfactory plasticity in Caenorhabditis elegans
description	Abstract In Caenorhabditis elegans, the AWC neurons are thought to deploy a cGMP signaling cascade in the detection of and response to AWC sensed odors. Prolonged exposure to an AWC sensed odor in the absence of food leads to reversible decreases in the animal’s attraction to that odor. This adaptation exhibits two stages referred to as short-term and long-term adaptation. Previously, the protein kinase G (PKG), EGL-4/PKG-1, was shown necessary for both stages of adaptation and phosphorylation of its target, the beta-type cyclic nucleotide gated (CNG) channel subunit, TAX-2, was implicated in the short term stage. Here we uncover a novel role for the CNG channel subunit, CNG-3, in short term adaptation. We demonstrate that CNG-3 is required in the AWC for adaptation to short (thirty minute) exposures of odor, and contains a candidate PKG phosphorylation site required to tune odor sensitivity. We also provide in vivo data suggesting that CNG-3 forms a complex with both TAX-2 and TAX-4 CNG channel subunits in AWC. Finally, we examine the physiology of different CNG channel subunit combinations.
format	article
author	Damien M. O’Halloran Svetlana Altshuler-Keylin Xiao-Dong Zhang Chao He Christopher Morales-Phan Yawei Yu Julia A. Kaye Chantal Brueggemann Tsung-Yu Chen Noelle D. L’Etoile
author_facet	Damien M. O’Halloran Svetlana Altshuler-Keylin Xiao-Dong Zhang Chao He Christopher Morales-Phan Yawei Yu Julia A. Kaye Chantal Brueggemann Tsung-Yu Chen Noelle D. L’Etoile
author_sort	Damien M. O’Halloran
title	Contribution of the cyclic nucleotide gated channel subunit, CNG-3, to olfactory plasticity in Caenorhabditis elegans
title_short	Contribution of the cyclic nucleotide gated channel subunit, CNG-3, to olfactory plasticity in Caenorhabditis elegans
title_full	Contribution of the cyclic nucleotide gated channel subunit, CNG-3, to olfactory plasticity in Caenorhabditis elegans
title_fullStr	Contribution of the cyclic nucleotide gated channel subunit, CNG-3, to olfactory plasticity in Caenorhabditis elegans
title_full_unstemmed	Contribution of the cyclic nucleotide gated channel subunit, CNG-3, to olfactory plasticity in Caenorhabditis elegans
title_sort	contribution of the cyclic nucleotide gated channel subunit, cng-3, to olfactory plasticity in caenorhabditis elegans
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9c0987815bae4206933dea42b37c80bd
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Contribution of the cyclic nucleotide gated channel subunit, CNG-3, to olfactory plasticity in Caenorhabditis elegans

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