CircRFX3 contributes to glioma progression through the circRFX3-miR-1179/miR-1229-VASP axis

Abstract Background Circular RNAs (circRNAs) are implicated in the carcinogenesis of human cancers. However, the functional roles of circRFX3 in glioma are not elucidated. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) assay was performed for the levels of circRFX3, RFX3, miR-117...

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Autores principales:	Hongli Li, Yiwei Zhang, Huiqin Song, Li Li
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Glioma circRFX3 miR-1179 miR-1229 VASP Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/9c0c154b81e84b00b1b57dae2792e6e9
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spelling	oai:doaj.org-article:9c0c154b81e84b00b1b57dae2792e6e92021-11-08T11:13:44ZCircRFX3 contributes to glioma progression through the circRFX3-miR-1179/miR-1229-VASP axis10.1186/s12935-021-02293-01475-2867https://doaj.org/article/9c0c154b81e84b00b1b57dae2792e6e92021-11-01T00:00:00Zhttps://doi.org/10.1186/s12935-021-02293-0https://doaj.org/toc/1475-2867Abstract Background Circular RNAs (circRNAs) are implicated in the carcinogenesis of human cancers. However, the functional roles of circRFX3 in glioma are not elucidated. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) assay was performed for the levels of circRFX3, RFX3, miR-1179, miR-1229 and vasodilator stimulated phosphoprotein (VASP). Actinomycin D assay and RNase R assay were employed to analyze the characteristics of circRFX3. Cell Counting Kit-8 (CCK-8) assay and colony formation assay were conducted for cell proliferation. Transwell assay was used for cell migration and invasion. Flow cytometry analysis was adopted for cell apoptosis. RNA pull-down assay, dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were employed to analyze the interaction between miR-1179/miR-1229 and circRFX3 or VASP. Western blot assay was conducted for VASP protein level. Murine xenograft model assay was used to investigate the role of circRFX3 in vivo. Results CircRFX3 level was increased in glioma tissues and cells. Knockdown of circRFX3 suppressed glioma cell proliferation, migration and invasion and promoted apoptosis in vitro and repressed tumorigenesis of glioma in vivo. MiR-1179 and miR-1229 were identified to be the targets of circRFX3. MiR-1179 or miR-1229 inhibition reversed the impacts of circRFX3 knockdown on glioma cell malignant behaviors. Additionally, VASP was demonstrated to be the target gene of miR-1179 and miR-1229, and VASP overexpression abolished the effect of circRFX3 knockdown on glioma cell progression. Conclusion CircRFX3 served as a tumor promoter in glioma via modulating miR-1179/miR-1229-VASP axis, which might provide a novel target for glioma therapy.Hongli LiYiwei ZhangHuiqin SongLi LiBMCarticleGliomacircRFX3miR-1179miR-1229VASPNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282CytologyQH573-671ENCancer Cell International, Vol 21, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Glioma circRFX3 miR-1179 miR-1229 VASP Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
spellingShingle	Glioma circRFX3 miR-1179 miR-1229 VASP Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671 Hongli Li Yiwei Zhang Huiqin Song Li Li CircRFX3 contributes to glioma progression through the circRFX3-miR-1179/miR-1229-VASP axis
description	Abstract Background Circular RNAs (circRNAs) are implicated in the carcinogenesis of human cancers. However, the functional roles of circRFX3 in glioma are not elucidated. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) assay was performed for the levels of circRFX3, RFX3, miR-1179, miR-1229 and vasodilator stimulated phosphoprotein (VASP). Actinomycin D assay and RNase R assay were employed to analyze the characteristics of circRFX3. Cell Counting Kit-8 (CCK-8) assay and colony formation assay were conducted for cell proliferation. Transwell assay was used for cell migration and invasion. Flow cytometry analysis was adopted for cell apoptosis. RNA pull-down assay, dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were employed to analyze the interaction between miR-1179/miR-1229 and circRFX3 or VASP. Western blot assay was conducted for VASP protein level. Murine xenograft model assay was used to investigate the role of circRFX3 in vivo. Results CircRFX3 level was increased in glioma tissues and cells. Knockdown of circRFX3 suppressed glioma cell proliferation, migration and invasion and promoted apoptosis in vitro and repressed tumorigenesis of glioma in vivo. MiR-1179 and miR-1229 were identified to be the targets of circRFX3. MiR-1179 or miR-1229 inhibition reversed the impacts of circRFX3 knockdown on glioma cell malignant behaviors. Additionally, VASP was demonstrated to be the target gene of miR-1179 and miR-1229, and VASP overexpression abolished the effect of circRFX3 knockdown on glioma cell progression. Conclusion CircRFX3 served as a tumor promoter in glioma via modulating miR-1179/miR-1229-VASP axis, which might provide a novel target for glioma therapy.
format	article
author	Hongli Li Yiwei Zhang Huiqin Song Li Li
author_facet	Hongli Li Yiwei Zhang Huiqin Song Li Li
author_sort	Hongli Li
title	CircRFX3 contributes to glioma progression through the circRFX3-miR-1179/miR-1229-VASP axis
title_short	CircRFX3 contributes to glioma progression through the circRFX3-miR-1179/miR-1229-VASP axis
title_full	CircRFX3 contributes to glioma progression through the circRFX3-miR-1179/miR-1229-VASP axis
title_fullStr	CircRFX3 contributes to glioma progression through the circRFX3-miR-1179/miR-1229-VASP axis
title_full_unstemmed	CircRFX3 contributes to glioma progression through the circRFX3-miR-1179/miR-1229-VASP axis
title_sort	circrfx3 contributes to glioma progression through the circrfx3-mir-1179/mir-1229-vasp axis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/9c0c154b81e84b00b1b57dae2792e6e9
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CircRFX3 contributes to glioma progression through the circRFX3-miR-1179/miR-1229-VASP axis

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