Revealing chemical processes and kinetics of drug action within single living cells via plasmonic Raman probes

Abstract Better understanding the drug action within cells may extend our knowledge on drug action mechanisms and promote new drugs discovery. Herein, we studied the processes of drug induced chemical changes on proteins and nucleic acids in human breast adenocarcinoma (MCF-7) cells via time-resolve...

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Autores principales:	Shan-Shan Li, Qi-Yuan Guan, Gang Meng, Xiao-Feng Chang, Ji-Wu Wei, Peng Wang, Bin Kang, Jing-Juan Xu, Hong-Yuan Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9c103e92b70b406c982b341cd8b68ebb
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spelling	oai:doaj.org-article:9c103e92b70b406c982b341cd8b68ebb2021-12-02T11:40:22ZRevealing chemical processes and kinetics of drug action within single living cells via plasmonic Raman probes10.1038/s41598-017-02510-92045-2322https://doaj.org/article/9c103e92b70b406c982b341cd8b68ebb2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02510-9https://doaj.org/toc/2045-2322Abstract Better understanding the drug action within cells may extend our knowledge on drug action mechanisms and promote new drugs discovery. Herein, we studied the processes of drug induced chemical changes on proteins and nucleic acids in human breast adenocarcinoma (MCF-7) cells via time-resolved plasmonic-enhanced Raman spectroscopy (PERS) in combination with principal component analysis (PCA). Using three popular chemotherapy drugs (fluorouracil, cisplatin and camptothecin) as models, chemical changes during drug action process were clearly discriminated. Reaction kinetics related to protein denaturation, conformational modification, DNA damage and their associated biomolecular events were calculated. Through rate constants and reaction delay times, the different action modes of these drugs could be distinguished. These results may provide vital insights into understanding the chemical reactions associated with drug-cell interactions.Shan-Shan LiQi-Yuan GuanGang MengXiao-Feng ChangJi-Wu WeiPeng WangBin KangJing-Juan XuHong-Yuan ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shan-Shan Li Qi-Yuan Guan Gang Meng Xiao-Feng Chang Ji-Wu Wei Peng Wang Bin Kang Jing-Juan Xu Hong-Yuan Chen Revealing chemical processes and kinetics of drug action within single living cells via plasmonic Raman probes
description	Abstract Better understanding the drug action within cells may extend our knowledge on drug action mechanisms and promote new drugs discovery. Herein, we studied the processes of drug induced chemical changes on proteins and nucleic acids in human breast adenocarcinoma (MCF-7) cells via time-resolved plasmonic-enhanced Raman spectroscopy (PERS) in combination with principal component analysis (PCA). Using three popular chemotherapy drugs (fluorouracil, cisplatin and camptothecin) as models, chemical changes during drug action process were clearly discriminated. Reaction kinetics related to protein denaturation, conformational modification, DNA damage and their associated biomolecular events were calculated. Through rate constants and reaction delay times, the different action modes of these drugs could be distinguished. These results may provide vital insights into understanding the chemical reactions associated with drug-cell interactions.
format	article
author	Shan-Shan Li Qi-Yuan Guan Gang Meng Xiao-Feng Chang Ji-Wu Wei Peng Wang Bin Kang Jing-Juan Xu Hong-Yuan Chen
author_facet	Shan-Shan Li Qi-Yuan Guan Gang Meng Xiao-Feng Chang Ji-Wu Wei Peng Wang Bin Kang Jing-Juan Xu Hong-Yuan Chen
author_sort	Shan-Shan Li
title	Revealing chemical processes and kinetics of drug action within single living cells via plasmonic Raman probes
title_short	Revealing chemical processes and kinetics of drug action within single living cells via plasmonic Raman probes
title_full	Revealing chemical processes and kinetics of drug action within single living cells via plasmonic Raman probes
title_fullStr	Revealing chemical processes and kinetics of drug action within single living cells via plasmonic Raman probes
title_full_unstemmed	Revealing chemical processes and kinetics of drug action within single living cells via plasmonic Raman probes
title_sort	revealing chemical processes and kinetics of drug action within single living cells via plasmonic raman probes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9c103e92b70b406c982b341cd8b68ebb
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Revealing chemical processes and kinetics of drug action within single living cells via plasmonic Raman probes

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