Coupling Root Diameter With Rooting Depth to Reveal the Heterogeneous Assembly of Root-Associated Bacterial Communities in Soybean

Root diameter and rooting depth lead to morphological and architectural heterogeneity of plant roots; however, little is known about their effects on root-associated microbial communities. Bacterial community assembly was explored across 156 samples from three rhizocompartments (the rhizosphere, rhi...

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Autores principales:	Wen Luo, Xiaoyu Zai, Jieyu Sun, Da Li, Yuanli Li, Guoqiang Li, Gehong Wei, Weimin Chen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	rhizocompartment rhizosphere microbiota legume plant shallow root system root traits Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9c13ab0a3a25413fbfa3e22b38550e2b
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spelling	oai:doaj.org-article:9c13ab0a3a25413fbfa3e22b38550e2b2021-12-03T07:10:04ZCoupling Root Diameter With Rooting Depth to Reveal the Heterogeneous Assembly of Root-Associated Bacterial Communities in Soybean1664-302X10.3389/fmicb.2021.783563https://doaj.org/article/9c13ab0a3a25413fbfa3e22b38550e2b2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.783563/fullhttps://doaj.org/toc/1664-302XRoot diameter and rooting depth lead to morphological and architectural heterogeneity of plant roots; however, little is known about their effects on root-associated microbial communities. Bacterial community assembly was explored across 156 samples from three rhizocompartments (the rhizosphere, rhizoplane, and endosphere) for different diameters (0.0–0.5 mm, 0.5–1.0 mm, 1.0–2.0 mm, and>2.0 mm) and depths (0–5 cm, 5–10 cm, 10–15 cm, and 15–20 cm) of soybean [Glycine max (L.) Merrill] root systems. The microbial communities of all samples were analyzed using amplicon sequencing of bacterial 16S rRNA genes. The results showed that root diameter significantly affected the rhizosphere and endosphere bacterial communities, while rooting depth significantly influenced the rhizosphere and rhizoplane bacterial communities. The bacterial alpha diversity decreased with increasing root diameter in all three rhizocompartments, and the diversity increased with increasing rooting depth only in the rhizoplane. Clearly, the hierarchical enrichment process of the bacterial community showed a change from the rhizosphere to the rhizoplane to the endosphere, and the bacterial enrichment was higher in thinner or deeper roots (except for the roots at a depth of 15–20 cm). Network analysis indicated that thinner or deeper roots led to higher bacterial network complexity. The core and keystone taxa associated with the specific root diameter class and rooting depth class harbored specific adaptation or selection strategies. Root diameter and rooting depth together affected the root-associated bacterial assembly and network complexity in the root system. Linking root traits to microbiota may enhance our understanding of plant root-microbe interactions and their role in developing environmentally resilient root ecosystems.Wen LuoXiaoyu ZaiJieyu SunDa LiYuanli LiGuoqiang LiGehong WeiWeimin ChenFrontiers Media S.A.articlerhizocompartmentrhizosphere microbiotalegume plantshallow root systemroot traitsMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rhizocompartment rhizosphere microbiota legume plant shallow root system root traits Microbiology QR1-502
spellingShingle	rhizocompartment rhizosphere microbiota legume plant shallow root system root traits Microbiology QR1-502 Wen Luo Xiaoyu Zai Jieyu Sun Da Li Yuanli Li Guoqiang Li Gehong Wei Weimin Chen Coupling Root Diameter With Rooting Depth to Reveal the Heterogeneous Assembly of Root-Associated Bacterial Communities in Soybean
description	Root diameter and rooting depth lead to morphological and architectural heterogeneity of plant roots; however, little is known about their effects on root-associated microbial communities. Bacterial community assembly was explored across 156 samples from three rhizocompartments (the rhizosphere, rhizoplane, and endosphere) for different diameters (0.0–0.5 mm, 0.5–1.0 mm, 1.0–2.0 mm, and>2.0 mm) and depths (0–5 cm, 5–10 cm, 10–15 cm, and 15–20 cm) of soybean [Glycine max (L.) Merrill] root systems. The microbial communities of all samples were analyzed using amplicon sequencing of bacterial 16S rRNA genes. The results showed that root diameter significantly affected the rhizosphere and endosphere bacterial communities, while rooting depth significantly influenced the rhizosphere and rhizoplane bacterial communities. The bacterial alpha diversity decreased with increasing root diameter in all three rhizocompartments, and the diversity increased with increasing rooting depth only in the rhizoplane. Clearly, the hierarchical enrichment process of the bacterial community showed a change from the rhizosphere to the rhizoplane to the endosphere, and the bacterial enrichment was higher in thinner or deeper roots (except for the roots at a depth of 15–20 cm). Network analysis indicated that thinner or deeper roots led to higher bacterial network complexity. The core and keystone taxa associated with the specific root diameter class and rooting depth class harbored specific adaptation or selection strategies. Root diameter and rooting depth together affected the root-associated bacterial assembly and network complexity in the root system. Linking root traits to microbiota may enhance our understanding of plant root-microbe interactions and their role in developing environmentally resilient root ecosystems.
format	article
author	Wen Luo Xiaoyu Zai Jieyu Sun Da Li Yuanli Li Guoqiang Li Gehong Wei Weimin Chen
author_facet	Wen Luo Xiaoyu Zai Jieyu Sun Da Li Yuanli Li Guoqiang Li Gehong Wei Weimin Chen
author_sort	Wen Luo
title	Coupling Root Diameter With Rooting Depth to Reveal the Heterogeneous Assembly of Root-Associated Bacterial Communities in Soybean
title_short	Coupling Root Diameter With Rooting Depth to Reveal the Heterogeneous Assembly of Root-Associated Bacterial Communities in Soybean
title_full	Coupling Root Diameter With Rooting Depth to Reveal the Heterogeneous Assembly of Root-Associated Bacterial Communities in Soybean
title_fullStr	Coupling Root Diameter With Rooting Depth to Reveal the Heterogeneous Assembly of Root-Associated Bacterial Communities in Soybean
title_full_unstemmed	Coupling Root Diameter With Rooting Depth to Reveal the Heterogeneous Assembly of Root-Associated Bacterial Communities in Soybean
title_sort	coupling root diameter with rooting depth to reveal the heterogeneous assembly of root-associated bacterial communities in soybean
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/9c13ab0a3a25413fbfa3e22b38550e2b
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Coupling Root Diameter With Rooting Depth to Reveal the Heterogeneous Assembly of Root-Associated Bacterial Communities in Soybean

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