LEDitSHAKE: a lighting system to optimize the secondary metabolite content of plant cell suspension cultures

Abstract Plant secondary metabolites are widely used in the food, cosmetic and pharmaceutical industries. They can be extracted from sterile grown plant cell suspension cultures, but yields and quality strongly depend on the cultivation environment, including optimal illumination. Current shaking in...

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Autores principales:	Ann-Katrin Beuel, Natalia Jablonka, Julia Heesel, Kevin Severin, Holger Spiegel, Stefan Rasche
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9c13c3099c8a4a458a0878ffe37c0711
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spelling	oai:doaj.org-article:9c13c3099c8a4a458a0878ffe37c07112021-12-05T12:13:55ZLEDitSHAKE: a lighting system to optimize the secondary metabolite content of plant cell suspension cultures10.1038/s41598-021-02762-62045-2322https://doaj.org/article/9c13c3099c8a4a458a0878ffe37c07112021-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02762-6https://doaj.org/toc/2045-2322Abstract Plant secondary metabolites are widely used in the food, cosmetic and pharmaceutical industries. They can be extracted from sterile grown plant cell suspension cultures, but yields and quality strongly depend on the cultivation environment, including optimal illumination. Current shaking incubators do not allow different light wavelengths, intensities and photoperiods to be tested in parallel. We therefore developed LEDitSHAKE, a system for multiplexed customized illumination within a single shaking incubator. We used 3D printing to integrate light-emitting diode assemblies into flask housings, allowing 12 different lighting conditions (spectrum, intensity and photoperiod) to be tested simultaneously. We did a proof of principle of LEDitSHAKE using the system to optimize anthocyanin production in grapevine cell suspension cultures. The effect of 24 different light compositions on the total anthocyanin content of grapevine cell suspension cultures was determined using a Design of Experiments approach. We predicted the optimal lighting conditions for the upregulation and downregulation of 30 anthocyanins and found that short-wavelength light (blue, UV) maximized the concentration of most anthocyanins, whereas long-wavelength light (red) had the opposite effect. Therefore our results demonstrate proof of principle that the LEDitSHAKE system is suitable for the optimization of processes based on plant cell suspension cultures.Ann-Katrin BeuelNatalia JablonkaJulia HeeselKevin SeverinHolger SpiegelStefan RascheNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ann-Katrin Beuel Natalia Jablonka Julia Heesel Kevin Severin Holger Spiegel Stefan Rasche LEDitSHAKE: a lighting system to optimize the secondary metabolite content of plant cell suspension cultures
description	Abstract Plant secondary metabolites are widely used in the food, cosmetic and pharmaceutical industries. They can be extracted from sterile grown plant cell suspension cultures, but yields and quality strongly depend on the cultivation environment, including optimal illumination. Current shaking incubators do not allow different light wavelengths, intensities and photoperiods to be tested in parallel. We therefore developed LEDitSHAKE, a system for multiplexed customized illumination within a single shaking incubator. We used 3D printing to integrate light-emitting diode assemblies into flask housings, allowing 12 different lighting conditions (spectrum, intensity and photoperiod) to be tested simultaneously. We did a proof of principle of LEDitSHAKE using the system to optimize anthocyanin production in grapevine cell suspension cultures. The effect of 24 different light compositions on the total anthocyanin content of grapevine cell suspension cultures was determined using a Design of Experiments approach. We predicted the optimal lighting conditions for the upregulation and downregulation of 30 anthocyanins and found that short-wavelength light (blue, UV) maximized the concentration of most anthocyanins, whereas long-wavelength light (red) had the opposite effect. Therefore our results demonstrate proof of principle that the LEDitSHAKE system is suitable for the optimization of processes based on plant cell suspension cultures.
format	article
author	Ann-Katrin Beuel Natalia Jablonka Julia Heesel Kevin Severin Holger Spiegel Stefan Rasche
author_facet	Ann-Katrin Beuel Natalia Jablonka Julia Heesel Kevin Severin Holger Spiegel Stefan Rasche
author_sort	Ann-Katrin Beuel
title	LEDitSHAKE: a lighting system to optimize the secondary metabolite content of plant cell suspension cultures
title_short	LEDitSHAKE: a lighting system to optimize the secondary metabolite content of plant cell suspension cultures
title_full	LEDitSHAKE: a lighting system to optimize the secondary metabolite content of plant cell suspension cultures
title_fullStr	LEDitSHAKE: a lighting system to optimize the secondary metabolite content of plant cell suspension cultures
title_full_unstemmed	LEDitSHAKE: a lighting system to optimize the secondary metabolite content of plant cell suspension cultures
title_sort	leditshake: a lighting system to optimize the secondary metabolite content of plant cell suspension cultures
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9c13c3099c8a4a458a0878ffe37c0711
work_keys_str_mv	AT annkatrinbeuel leditshakealightingsystemtooptimizethesecondarymetabolitecontentofplantcellsuspensioncultures AT nataliajablonka leditshakealightingsystemtooptimizethesecondarymetabolitecontentofplantcellsuspensioncultures AT juliaheesel leditshakealightingsystemtooptimizethesecondarymetabolitecontentofplantcellsuspensioncultures AT kevinseverin leditshakealightingsystemtooptimizethesecondarymetabolitecontentofplantcellsuspensioncultures AT holgerspiegel leditshakealightingsystemtooptimizethesecondarymetabolitecontentofplantcellsuspensioncultures AT stefanrasche leditshakealightingsystemtooptimizethesecondarymetabolitecontentofplantcellsuspensioncultures
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LEDitSHAKE: a lighting system to optimize the secondary metabolite content of plant cell suspension cultures

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