Triolein emulsion enhances temozolomide brain delivery: an experimental study in rats

Purpose To evaluate the enhancement of temozolomide (TMZ) delivery in the rat brain using a triolein emulsion. Materials and Methods Rats were divided into the five groups as following: group 1 (negative control), group 2 (treated with triolein emulsion and TMZ 20 mg/kg), and group 3 (TMZ 20 mg/kg t...

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Autores principales:	Won-Bae Seung, Seung Heon Cha, Hak Jin Kim, Seon Hee Choi, Juho Lee, Dongmin Kwak, Kim Hyun Woo, Jin-Wook You, Yong-Woo Kim, Sang Kyoon Kim, Da-Sol Lee
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	triolein emulsion temozolomide bbb drug delivery liquid chromatography Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/9c17b75ffb844f1cb05c47531452b26b
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spelling	oai:doaj.org-article:9c17b75ffb844f1cb05c47531452b26b2021-11-11T14:23:41ZTriolein emulsion enhances temozolomide brain delivery: an experimental study in rats1071-75441521-046410.1080/10717544.2021.1998247https://doaj.org/article/9c17b75ffb844f1cb05c47531452b26b2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/10717544.2021.1998247https://doaj.org/toc/1071-7544https://doaj.org/toc/1521-0464Purpose To evaluate the enhancement of temozolomide (TMZ) delivery in the rat brain using a triolein emulsion. Materials and Methods Rats were divided into the five groups as following: group 1 (negative control), group 2 (treated with triolein emulsion and TMZ 20 mg/kg), and group 3 (TMZ 20 mg/kg treatment without triolein), group 4 (treated with triolein emulsion and TMZ 10 mg/kg), and group 5 (TMZ 10 mg/kg treatment without triolein). Triolein emulsion was infused into the right common carotid artery. One hour later, the TMZ concentration was evaluated quantitatively and qualitatively using high-performance liquid chromatography (HPLC-MS) and desorption electrospray ionization mass spectrometry (DESI-MS) imaging, respectively. The concentration ratios of the ipsilateral to contralateral hemisphere in each group were determined and the statistical analysis was conducted using an unpaired t-test. Results Quantitatively, the TMZ concentration ratio of the ipsilateral to the control hemisphere was 2.41 and 1.13 in groups 2 and 3, and were 2.49 and 1.14 in groups 4 and 5, respectively. Thus, the TMZ signal intensities of TMZ in group 2 and 4 were statistically high in the ipsilateral hemispheres. Qualitatively, the signal intensity of TMZ was remarkably high in the ipsilateral hemisphere in group 2 and 4. Conclusions The triolein emulsion efficiently opened the blood-brain barrier and could provide a potential new strategy to enhance the therapeutic effect of TMZ. HPLC-MS and DESI-MS imaging were shown to be suitable for analyses of enhancement of brain TMZ concentrations.Won-Bae SeungSeung Heon ChaHak Jin KimSeon Hee ChoiJuho LeeDongmin KwakKim Hyun WooJin-Wook YouYong-Woo KimSang Kyoon KimDa-Sol LeeTaylor & Francis Grouparticletriolein emulsiontemozolomidebbbdrug deliveryliquid chromatographyTherapeutics. PharmacologyRM1-950ENDrug Delivery, Vol 28, Iss 1, Pp 2373-2382 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	triolein emulsion temozolomide bbb drug delivery liquid chromatography Therapeutics. Pharmacology RM1-950
spellingShingle	triolein emulsion temozolomide bbb drug delivery liquid chromatography Therapeutics. Pharmacology RM1-950 Won-Bae Seung Seung Heon Cha Hak Jin Kim Seon Hee Choi Juho Lee Dongmin Kwak Kim Hyun Woo Jin-Wook You Yong-Woo Kim Sang Kyoon Kim Da-Sol Lee Triolein emulsion enhances temozolomide brain delivery: an experimental study in rats
description	Purpose To evaluate the enhancement of temozolomide (TMZ) delivery in the rat brain using a triolein emulsion. Materials and Methods Rats were divided into the five groups as following: group 1 (negative control), group 2 (treated with triolein emulsion and TMZ 20 mg/kg), and group 3 (TMZ 20 mg/kg treatment without triolein), group 4 (treated with triolein emulsion and TMZ 10 mg/kg), and group 5 (TMZ 10 mg/kg treatment without triolein). Triolein emulsion was infused into the right common carotid artery. One hour later, the TMZ concentration was evaluated quantitatively and qualitatively using high-performance liquid chromatography (HPLC-MS) and desorption electrospray ionization mass spectrometry (DESI-MS) imaging, respectively. The concentration ratios of the ipsilateral to contralateral hemisphere in each group were determined and the statistical analysis was conducted using an unpaired t-test. Results Quantitatively, the TMZ concentration ratio of the ipsilateral to the control hemisphere was 2.41 and 1.13 in groups 2 and 3, and were 2.49 and 1.14 in groups 4 and 5, respectively. Thus, the TMZ signal intensities of TMZ in group 2 and 4 were statistically high in the ipsilateral hemispheres. Qualitatively, the signal intensity of TMZ was remarkably high in the ipsilateral hemisphere in group 2 and 4. Conclusions The triolein emulsion efficiently opened the blood-brain barrier and could provide a potential new strategy to enhance the therapeutic effect of TMZ. HPLC-MS and DESI-MS imaging were shown to be suitable for analyses of enhancement of brain TMZ concentrations.
format	article
author	Won-Bae Seung Seung Heon Cha Hak Jin Kim Seon Hee Choi Juho Lee Dongmin Kwak Kim Hyun Woo Jin-Wook You Yong-Woo Kim Sang Kyoon Kim Da-Sol Lee
author_facet	Won-Bae Seung Seung Heon Cha Hak Jin Kim Seon Hee Choi Juho Lee Dongmin Kwak Kim Hyun Woo Jin-Wook You Yong-Woo Kim Sang Kyoon Kim Da-Sol Lee
author_sort	Won-Bae Seung
title	Triolein emulsion enhances temozolomide brain delivery: an experimental study in rats
title_short	Triolein emulsion enhances temozolomide brain delivery: an experimental study in rats
title_full	Triolein emulsion enhances temozolomide brain delivery: an experimental study in rats
title_fullStr	Triolein emulsion enhances temozolomide brain delivery: an experimental study in rats
title_full_unstemmed	Triolein emulsion enhances temozolomide brain delivery: an experimental study in rats
title_sort	triolein emulsion enhances temozolomide brain delivery: an experimental study in rats
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/9c17b75ffb844f1cb05c47531452b26b
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Triolein emulsion enhances temozolomide brain delivery: an experimental study in rats

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