Specific visualization of glioma cells in living low-grade tumor tissue.

<h4>Background</h4>The current therapy of malignant gliomas is based on surgical resection, radio-chemotherapy and chemotherapy. Recent retrospective case-series have highlighted the significance of the extent of resection as a prognostic factor predicting the course of the disease. Comp...

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Autores principales:	Sven R Kantelhardt, Wouter Caarls, Anthony H B de Vries, Guy M Hagen, Thomas M Jovin, Walter Schulz-Schaeffer, Veit Rohde, Alf Giese, Donna J Arndt-Jovin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9c184c9dde40499fa32bef8084b01795
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spelling	oai:doaj.org-article:9c184c9dde40499fa32bef8084b017952021-12-02T20:20:21ZSpecific visualization of glioma cells in living low-grade tumor tissue.1932-620310.1371/journal.pone.0011323https://doaj.org/article/9c184c9dde40499fa32bef8084b017952010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20614029/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The current therapy of malignant gliomas is based on surgical resection, radio-chemotherapy and chemotherapy. Recent retrospective case-series have highlighted the significance of the extent of resection as a prognostic factor predicting the course of the disease. Complete resection in low-grade gliomas that show no MRI-enhanced images are especially difficult. The aim in this study was to develop a robust, specific, new fluorescent probe for glioma cells that is easy to apply to live tumor biopsies and could identify tumor cells from normal brain cells at all levels of magnification.<h4>Methodology/principal findings</h4>In this investigation we employed brightly fluorescent, photostable quantum dots (QDs) to specifically target epidermal growth factor receptor (EGFR) that is upregulated in many gliomas. Living glioma and normal cells or tissue biopsies were incubated with QDs coupled to EGF and/or monoclonal antibodies against EGFR for 30 minutes, washed and imaged. The data include results from cell-culture, animal model and ex vivo human tumor biopsies of both low-grade and high-grade gliomas and show high probe specificity. Tumor cells could be visualized from the macroscopic to single cell level with contrast ratios as high as 1000: 1 compared to normal brain tissue.<h4>Conclusions/significance</h4>The ability of the targeted probes to clearly distinguish tumor cells in low-grade tumor biopsies, where no enhanced MRI image was obtained, demonstrates the great potential of the method. We propose that future application of specifically targeted fluorescent particles during surgery could allow intraoperative guidance for the removal of residual tumor cells from the resection cavity and thus increase patient survival.Sven R KantelhardtWouter CaarlsAnthony H B de VriesGuy M HagenThomas M JovinWalter Schulz-SchaefferVeit RohdeAlf GieseDonna J Arndt-JovinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 6, p e11323 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sven R Kantelhardt Wouter Caarls Anthony H B de Vries Guy M Hagen Thomas M Jovin Walter Schulz-Schaeffer Veit Rohde Alf Giese Donna J Arndt-Jovin Specific visualization of glioma cells in living low-grade tumor tissue.
description	<h4>Background</h4>The current therapy of malignant gliomas is based on surgical resection, radio-chemotherapy and chemotherapy. Recent retrospective case-series have highlighted the significance of the extent of resection as a prognostic factor predicting the course of the disease. Complete resection in low-grade gliomas that show no MRI-enhanced images are especially difficult. The aim in this study was to develop a robust, specific, new fluorescent probe for glioma cells that is easy to apply to live tumor biopsies and could identify tumor cells from normal brain cells at all levels of magnification.<h4>Methodology/principal findings</h4>In this investigation we employed brightly fluorescent, photostable quantum dots (QDs) to specifically target epidermal growth factor receptor (EGFR) that is upregulated in many gliomas. Living glioma and normal cells or tissue biopsies were incubated with QDs coupled to EGF and/or monoclonal antibodies against EGFR for 30 minutes, washed and imaged. The data include results from cell-culture, animal model and ex vivo human tumor biopsies of both low-grade and high-grade gliomas and show high probe specificity. Tumor cells could be visualized from the macroscopic to single cell level with contrast ratios as high as 1000: 1 compared to normal brain tissue.<h4>Conclusions/significance</h4>The ability of the targeted probes to clearly distinguish tumor cells in low-grade tumor biopsies, where no enhanced MRI image was obtained, demonstrates the great potential of the method. We propose that future application of specifically targeted fluorescent particles during surgery could allow intraoperative guidance for the removal of residual tumor cells from the resection cavity and thus increase patient survival.
format	article
author	Sven R Kantelhardt Wouter Caarls Anthony H B de Vries Guy M Hagen Thomas M Jovin Walter Schulz-Schaeffer Veit Rohde Alf Giese Donna J Arndt-Jovin
author_facet	Sven R Kantelhardt Wouter Caarls Anthony H B de Vries Guy M Hagen Thomas M Jovin Walter Schulz-Schaeffer Veit Rohde Alf Giese Donna J Arndt-Jovin
author_sort	Sven R Kantelhardt
title	Specific visualization of glioma cells in living low-grade tumor tissue.
title_short	Specific visualization of glioma cells in living low-grade tumor tissue.
title_full	Specific visualization of glioma cells in living low-grade tumor tissue.
title_fullStr	Specific visualization of glioma cells in living low-grade tumor tissue.
title_full_unstemmed	Specific visualization of glioma cells in living low-grade tumor tissue.
title_sort	specific visualization of glioma cells in living low-grade tumor tissue.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/9c184c9dde40499fa32bef8084b01795
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Specific visualization of glioma cells in living low-grade tumor tissue.

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