Increased RNA virus population diversity improves adaptability

Abstract The replication machinery of most RNA viruses lacks proofreading mechanisms. As a result, RNA virus populations harbor a large amount of genetic diversity that confers them the ability to rapidly adapt to changes in their environment. In this work, we investigate whether further increasing...

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Autores principales:	Florian Mattenberger, Marina Vila-Nistal, Ron Geller
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9c21002cc93c401dbeedb77867896e13
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spelling	oai:doaj.org-article:9c21002cc93c401dbeedb77867896e132021-12-02T11:45:04ZIncreased RNA virus population diversity improves adaptability10.1038/s41598-021-86375-z2045-2322https://doaj.org/article/9c21002cc93c401dbeedb77867896e132021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86375-zhttps://doaj.org/toc/2045-2322Abstract The replication machinery of most RNA viruses lacks proofreading mechanisms. As a result, RNA virus populations harbor a large amount of genetic diversity that confers them the ability to rapidly adapt to changes in their environment. In this work, we investigate whether further increasing the initial population diversity of a model RNA virus can improve adaptation to a single selection pressure, thermal inactivation. For this, we experimentally increased the diversity of coxsackievirus B3 (CVB3) populations across the capsid region. We then compared the ability of these high diversity CVB3 populations to achieve resistance to thermal inactivation relative to standard CVB3 populations in an experimental evolution setting. We find that viral populations with high diversity are better able to achieve resistance to thermal inactivation at both the temperature employed during experimental evolution as well as at a more extreme temperature. Moreover, we identify mutations in the CVB3 capsid that confer resistance to thermal inactivation, finding significant mutational epistasis. Our results indicate that even naturally diverse RNA virus populations can benefit from experimental augmentation of population diversity for optimal adaptation and support the use of such viral populations in directed evolution efforts that aim to select viruses with desired characteristics.Florian MattenbergerMarina Vila-NistalRon GellerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Florian Mattenberger Marina Vila-Nistal Ron Geller Increased RNA virus population diversity improves adaptability
description	Abstract The replication machinery of most RNA viruses lacks proofreading mechanisms. As a result, RNA virus populations harbor a large amount of genetic diversity that confers them the ability to rapidly adapt to changes in their environment. In this work, we investigate whether further increasing the initial population diversity of a model RNA virus can improve adaptation to a single selection pressure, thermal inactivation. For this, we experimentally increased the diversity of coxsackievirus B3 (CVB3) populations across the capsid region. We then compared the ability of these high diversity CVB3 populations to achieve resistance to thermal inactivation relative to standard CVB3 populations in an experimental evolution setting. We find that viral populations with high diversity are better able to achieve resistance to thermal inactivation at both the temperature employed during experimental evolution as well as at a more extreme temperature. Moreover, we identify mutations in the CVB3 capsid that confer resistance to thermal inactivation, finding significant mutational epistasis. Our results indicate that even naturally diverse RNA virus populations can benefit from experimental augmentation of population diversity for optimal adaptation and support the use of such viral populations in directed evolution efforts that aim to select viruses with desired characteristics.
format	article
author	Florian Mattenberger Marina Vila-Nistal Ron Geller
author_facet	Florian Mattenberger Marina Vila-Nistal Ron Geller
author_sort	Florian Mattenberger
title	Increased RNA virus population diversity improves adaptability
title_short	Increased RNA virus population diversity improves adaptability
title_full	Increased RNA virus population diversity improves adaptability
title_fullStr	Increased RNA virus population diversity improves adaptability
title_full_unstemmed	Increased RNA virus population diversity improves adaptability
title_sort	increased rna virus population diversity improves adaptability
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9c21002cc93c401dbeedb77867896e13
work_keys_str_mv	AT florianmattenberger increasedrnaviruspopulationdiversityimprovesadaptability AT marinavilanistal increasedrnaviruspopulationdiversityimprovesadaptability AT rongeller increasedrnaviruspopulationdiversityimprovesadaptability
_version_	1718395297742192640

Increased RNA virus population diversity improves adaptability

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