Toxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in Caenorhabditis elegans
Ning Wang,1 Hui Wang,2 Chengchun Tang,3 Shijun Lei,1 Wanqing Shen,1 Cong Wang,1 Guobin Wang,4 Zheng Wang,1,4 Lin Wang1,5 1Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, 2Department of Medical Genetics, Tongji Medical College, Huazhong University of Science and Tec...
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Dove Medical Press
2017
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oai:doaj.org-article:9c2d6cc086044ffcb7830923f86ea5c92021-12-02T00:46:40ZToxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in Caenorhabditis elegans1178-2013https://doaj.org/article/9c2d6cc086044ffcb7830923f86ea5c92017-08-01T00:00:00Zhttps://www.dovepress.com/toxicity-evaluation-of-boron-nitride-nanospheres-and-water-soluble-bor-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ning Wang,1 Hui Wang,2 Chengchun Tang,3 Shijun Lei,1 Wanqing Shen,1 Cong Wang,1 Guobin Wang,4 Zheng Wang,1,4 Lin Wang1,5 1Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, 2Department of Medical Genetics, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 3Boron Nitride Research Center, School of Materials Science and Engineering, Hebei University of Technology, Tianjin, 4Department of Gastrointestinal Surgery, 5Department of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China Abstract: Boron nitride (BN) nanomaterials have been increasingly explored for potential biological applications. However, their toxicity remains poorly understood. Using Caenorhabditis elegans as a whole-animal model for toxicity analysis of two representative types of BN nanomaterials – BN nanospheres (BNNSs) and highly water-soluble BN nanomaterial (named BN-800-2) – we found that BNNSs overall toxicity was less than soluble BN-800-2 with irregular shapes. The concentration thresholds for BNNSs and BN-800-2 were 100 µg·mL-1 and 10 µg·mL-1, respectively. Above this concentration, both delayed growth, decreased life span, reduced progeny, retarded locomotion behavior, and changed the expression of phenotype-related genes to various extents. BNNSs and BN-800-2 increased oxidative stress levels in C. elegans by promoting reactive oxygen species production. Our results further showed that oxidative stress response and MAPK signaling-related genes, such as GAS1, SOD2, SOD3, MEK1, and PMK1, might be key factors for reactive oxygen species production and toxic responses to BNNSs and BN-800-2 exposure. Together, our results suggest that when concentrations are lower than 10 µg·mL-1, BNNSs are more biocompatible than BN-800-2 and are potentially biocompatible material. Keywords: boron nitride nanomaterials, Caenorhabditis elegans, nanotoxicology, reactive oxygen species, toxicityWang NWang HTang CLei SShen WWang CWang GWang ZWang LDove Medical Pressarticleboron nitride nanomaterialsCaenorhabditis elegansnanotoxicologyreactive oxygen speciestoxicityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 5941-5957 (2017) |
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boron nitride nanomaterials Caenorhabditis elegans nanotoxicology reactive oxygen species toxicity Medicine (General) R5-920 |
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boron nitride nanomaterials Caenorhabditis elegans nanotoxicology reactive oxygen species toxicity Medicine (General) R5-920 Wang N Wang H Tang C Lei S Shen W Wang C Wang G Wang Z Wang L Toxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in Caenorhabditis elegans |
| description |
Ning Wang,1 Hui Wang,2 Chengchun Tang,3 Shijun Lei,1 Wanqing Shen,1 Cong Wang,1 Guobin Wang,4 Zheng Wang,1,4 Lin Wang1,5 1Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, 2Department of Medical Genetics, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 3Boron Nitride Research Center, School of Materials Science and Engineering, Hebei University of Technology, Tianjin, 4Department of Gastrointestinal Surgery, 5Department of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China Abstract: Boron nitride (BN) nanomaterials have been increasingly explored for potential biological applications. However, their toxicity remains poorly understood. Using Caenorhabditis elegans as a whole-animal model for toxicity analysis of two representative types of BN nanomaterials – BN nanospheres (BNNSs) and highly water-soluble BN nanomaterial (named BN-800-2) – we found that BNNSs overall toxicity was less than soluble BN-800-2 with irregular shapes. The concentration thresholds for BNNSs and BN-800-2 were 100 µg·mL-1 and 10 µg·mL-1, respectively. Above this concentration, both delayed growth, decreased life span, reduced progeny, retarded locomotion behavior, and changed the expression of phenotype-related genes to various extents. BNNSs and BN-800-2 increased oxidative stress levels in C. elegans by promoting reactive oxygen species production. Our results further showed that oxidative stress response and MAPK signaling-related genes, such as GAS1, SOD2, SOD3, MEK1, and PMK1, might be key factors for reactive oxygen species production and toxic responses to BNNSs and BN-800-2 exposure. Together, our results suggest that when concentrations are lower than 10 µg·mL-1, BNNSs are more biocompatible than BN-800-2 and are potentially biocompatible material. Keywords: boron nitride nanomaterials, Caenorhabditis elegans, nanotoxicology, reactive oxygen species, toxicity |
| format |
article |
| author |
Wang N Wang H Tang C Lei S Shen W Wang C Wang G Wang Z Wang L |
| author_facet |
Wang N Wang H Tang C Lei S Shen W Wang C Wang G Wang Z Wang L |
| author_sort |
Wang N |
| title |
Toxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in Caenorhabditis elegans |
| title_short |
Toxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in Caenorhabditis elegans |
| title_full |
Toxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in Caenorhabditis elegans |
| title_fullStr |
Toxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in Caenorhabditis elegans |
| title_full_unstemmed |
Toxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in Caenorhabditis elegans |
| title_sort |
toxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in caenorhabditis elegans |
| publisher |
Dove Medical Press |
| publishDate |
2017 |
| url |
https://doaj.org/article/9c2d6cc086044ffcb7830923f86ea5c9 |
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