Bacillus-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens

In this article, the supernatant of the soil-borne pathogen Bacillus mn14 was used as the catalyst for the synthesis of AgNPs. The antibacterial and antifungal activity of Bs-AgNPs was evaluated, in which S. viridans and R. solani showed susce...

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Autores principales:	Nivedhitha Kabeerdass, Ahmed Al Otaibi, Manikandan Rajendran, Ayyar Manikandan, Heba A. Kashmery, Mohammed M. Rahman, P. Madhu, Anish Khan, Abdullah M. Asiri, Maghimaa Mathanmohun
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antibacterial antifungal AgNPs paddy plant growth promotion Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/9c2e3e52e0164a3ea849047ea15aabf5
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spelling	oai:doaj.org-article:9c2e3e52e0164a3ea849047ea15aabf52021-11-25T16:22:59Z<i>Bacillus</i>-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens10.3390/antibiotics101113342079-6382https://doaj.org/article/9c2e3e52e0164a3ea849047ea15aabf52021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1334https://doaj.org/toc/2079-6382In this article, the supernatant of the soil-borne pathogen <i>Bacillus</i> mn14 was used as the catalyst for the synthesis of AgNPs. The antibacterial and antifungal activity of Bs-AgNPs was evaluated, in which <i>S. viridans</i> and <i>R. solani</i> showed susceptibility at 70 µL and 100 µL concentrations. Enzyme properties of the isolates, according to minimal inhibitory action and a growth-enhancing hormone–indole acetic acid (IAA) study of the isolates, were expressed in TLC as a purple color with an Rf value of 0.7. UV/Vis spectroscopy revealed the presence of small-sized AgNPs, with a surface plasmon resonance (SPR) peak at 450 nm. The particle size analyzer identified the average diameter of the particles as 40.2 nm. The X-ray diffraction study confirmed the crystalline nature and face-centered cubic type of the silver nanoparticle. Scanning electron microscopy characterized the globular, small, round shape of the silver nanoparticle. AFM revealed the two-dimensional topology of the silver nanoparticle with a characteristic size ranging around 50 nm. Confocal microscopy showed the cell-wall disruption of <i>S. viridans</i> treated with Bs-AgNPs. High-content screening and compound microscopy revealed the destruction of mycelia of <i>R. solani</i> after exposure to Bs-AgNPs. Furthermore, the Bs-AgNPs cured sheath blight disease by reducing lesion length and enhancing root and shoot length in <i>Oryza sativa</i> seeds. This soil-borne pathogen <i>Bacillus</i>-mediated synthesis approach of AgNPs appears to be cost-efficient, ecofriendly, and farmer-friendly, representing an easy way of providing valuable nutritious edibles in the future.Nivedhitha KabeerdassAhmed Al OtaibiManikandan RajendranAyyar ManikandanHeba A. KashmeryMohammed M. RahmanP. MadhuAnish KhanAbdullah M. AsiriMaghimaa MathanmohunMDPI AGarticleantibacterialantifungalAgNPspaddy plant growth promotionTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1334, p 1334 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibacterial antifungal AgNPs paddy plant growth promotion Therapeutics. Pharmacology RM1-950
spellingShingle	antibacterial antifungal AgNPs paddy plant growth promotion Therapeutics. Pharmacology RM1-950 Nivedhitha Kabeerdass Ahmed Al Otaibi Manikandan Rajendran Ayyar Manikandan Heba A. Kashmery Mohammed M. Rahman P. Madhu Anish Khan Abdullah M. Asiri Maghimaa Mathanmohun <i>Bacillus</i>-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens
description	In this article, the supernatant of the soil-borne pathogen <i>Bacillus</i> mn14 was used as the catalyst for the synthesis of AgNPs. The antibacterial and antifungal activity of Bs-AgNPs was evaluated, in which <i>S. viridans</i> and <i>R. solani</i> showed susceptibility at 70 µL and 100 µL concentrations. Enzyme properties of the isolates, according to minimal inhibitory action and a growth-enhancing hormone–indole acetic acid (IAA) study of the isolates, were expressed in TLC as a purple color with an Rf value of 0.7. UV/Vis spectroscopy revealed the presence of small-sized AgNPs, with a surface plasmon resonance (SPR) peak at 450 nm. The particle size analyzer identified the average diameter of the particles as 40.2 nm. The X-ray diffraction study confirmed the crystalline nature and face-centered cubic type of the silver nanoparticle. Scanning electron microscopy characterized the globular, small, round shape of the silver nanoparticle. AFM revealed the two-dimensional topology of the silver nanoparticle with a characteristic size ranging around 50 nm. Confocal microscopy showed the cell-wall disruption of <i>S. viridans</i> treated with Bs-AgNPs. High-content screening and compound microscopy revealed the destruction of mycelia of <i>R. solani</i> after exposure to Bs-AgNPs. Furthermore, the Bs-AgNPs cured sheath blight disease by reducing lesion length and enhancing root and shoot length in <i>Oryza sativa</i> seeds. This soil-borne pathogen <i>Bacillus</i>-mediated synthesis approach of AgNPs appears to be cost-efficient, ecofriendly, and farmer-friendly, representing an easy way of providing valuable nutritious edibles in the future.
format	article
author	Nivedhitha Kabeerdass Ahmed Al Otaibi Manikandan Rajendran Ayyar Manikandan Heba A. Kashmery Mohammed M. Rahman P. Madhu Anish Khan Abdullah M. Asiri Maghimaa Mathanmohun
author_facet	Nivedhitha Kabeerdass Ahmed Al Otaibi Manikandan Rajendran Ayyar Manikandan Heba A. Kashmery Mohammed M. Rahman P. Madhu Anish Khan Abdullah M. Asiri Maghimaa Mathanmohun
author_sort	Nivedhitha Kabeerdass
title	<i>Bacillus</i>-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens
title_short	<i>Bacillus</i>-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens
title_full	<i>Bacillus</i>-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens
title_fullStr	<i>Bacillus</i>-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens
title_full_unstemmed	<i>Bacillus</i>-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens
title_sort	<i>bacillus</i>-mediated silver nanoparticle synthesis and its antagonistic activity against bacterial and fungal pathogens
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9c2e3e52e0164a3ea849047ea15aabf5
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<i>Bacillus</i>-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens

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