Polyclonal alpaca antibodies protect against hantavirus pulmonary syndrome in a lethal Syrian hamster model

Abstract The use of antibody-based therapies for the treatment of high consequence viral pathogens has gained interest over the last fifteen years. Here, we sought to evaluate the use of unique camelid-based IgG antibodies to prevent lethal hantavirus pulmonary syndrome (HPS) in Syrian hamsters. Usi...

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Autores principales:	Patrycja Sroga, Angela Sloan, Bryce M. Warner, Kevin Tierney, Jocelyne Lew, Guodong Liu, Michael Chan, Yvon Deschambault, Derek R. Stein, Geoff Soule, Logan Banadyga, Darryl Falzarano, David Safronetz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9c32e3c2b2f64cb28285301295ae86fb
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spelling	oai:doaj.org-article:9c32e3c2b2f64cb28285301295ae86fb2021-12-02T17:51:16ZPolyclonal alpaca antibodies protect against hantavirus pulmonary syndrome in a lethal Syrian hamster model10.1038/s41598-021-96884-62045-2322https://doaj.org/article/9c32e3c2b2f64cb28285301295ae86fb2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96884-6https://doaj.org/toc/2045-2322Abstract The use of antibody-based therapies for the treatment of high consequence viral pathogens has gained interest over the last fifteen years. Here, we sought to evaluate the use of unique camelid-based IgG antibodies to prevent lethal hantavirus pulmonary syndrome (HPS) in Syrian hamsters. Using purified, polyclonal IgG antibodies generated in DNA-immunized alpacas, we demonstrate that post-exposure treatments reduced viral burdens and organ-specific pathology associated with lethal HPS. Antibody treated animals did not exhibit signs of disease and were completely protected. The unique structures and properties, particularly the reduced size, distinct paratope formation and increased solubility of camelid antibodies, in combination with this study support further pre-clinical evaluation of heavy-chain only antibodies for treatment of severe respiratory diseases, including HPS.Patrycja SrogaAngela SloanBryce M. WarnerKevin TierneyJocelyne LewGuodong LiuMichael ChanYvon DeschambaultDerek R. SteinGeoff SouleLogan BanadygaDarryl FalzaranoDavid SafronetzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patrycja Sroga Angela Sloan Bryce M. Warner Kevin Tierney Jocelyne Lew Guodong Liu Michael Chan Yvon Deschambault Derek R. Stein Geoff Soule Logan Banadyga Darryl Falzarano David Safronetz Polyclonal alpaca antibodies protect against hantavirus pulmonary syndrome in a lethal Syrian hamster model
description	Abstract The use of antibody-based therapies for the treatment of high consequence viral pathogens has gained interest over the last fifteen years. Here, we sought to evaluate the use of unique camelid-based IgG antibodies to prevent lethal hantavirus pulmonary syndrome (HPS) in Syrian hamsters. Using purified, polyclonal IgG antibodies generated in DNA-immunized alpacas, we demonstrate that post-exposure treatments reduced viral burdens and organ-specific pathology associated with lethal HPS. Antibody treated animals did not exhibit signs of disease and were completely protected. The unique structures and properties, particularly the reduced size, distinct paratope formation and increased solubility of camelid antibodies, in combination with this study support further pre-clinical evaluation of heavy-chain only antibodies for treatment of severe respiratory diseases, including HPS.
format	article
author	Patrycja Sroga Angela Sloan Bryce M. Warner Kevin Tierney Jocelyne Lew Guodong Liu Michael Chan Yvon Deschambault Derek R. Stein Geoff Soule Logan Banadyga Darryl Falzarano David Safronetz
author_facet	Patrycja Sroga Angela Sloan Bryce M. Warner Kevin Tierney Jocelyne Lew Guodong Liu Michael Chan Yvon Deschambault Derek R. Stein Geoff Soule Logan Banadyga Darryl Falzarano David Safronetz
author_sort	Patrycja Sroga
title	Polyclonal alpaca antibodies protect against hantavirus pulmonary syndrome in a lethal Syrian hamster model
title_short	Polyclonal alpaca antibodies protect against hantavirus pulmonary syndrome in a lethal Syrian hamster model
title_full	Polyclonal alpaca antibodies protect against hantavirus pulmonary syndrome in a lethal Syrian hamster model
title_fullStr	Polyclonal alpaca antibodies protect against hantavirus pulmonary syndrome in a lethal Syrian hamster model
title_full_unstemmed	Polyclonal alpaca antibodies protect against hantavirus pulmonary syndrome in a lethal Syrian hamster model
title_sort	polyclonal alpaca antibodies protect against hantavirus pulmonary syndrome in a lethal syrian hamster model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9c32e3c2b2f64cb28285301295ae86fb
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Polyclonal alpaca antibodies protect against hantavirus pulmonary syndrome in a lethal Syrian hamster model

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