Massively parallel digital transcriptional profiling of single cells

Single-cell gene expression analysis is challenging. This work describes a new droplet-based single cell RNA-seq platform capable of processing tens of thousands of cells across 8 independent samples in minutes, and demonstrates cellular subtypes and host–donor chimerism in transplant patients.

Guardado en:

Detalles Bibliográficos
Autores principales:	Grace X. Y. Zheng, Jessica M. Terry, Phillip Belgrader, Paul Ryvkin, Zachary W. Bent, Ryan Wilson, Solongo B. Ziraldo, Tobias D. Wheeler, Geoff P. McDermott, Junjie Zhu, Mark T. Gregory, Joe Shuga, Luz Montesclaros, Jason G. Underwood, Donald A. Masquelier, Stefanie Y. Nishimura, Michael Schnall-Levin, Paul W. Wyatt, Christopher M. Hindson, Rajiv Bharadwaj, Alexander Wong, Kevin D. Ness, Lan W. Beppu, H. Joachim Deeg, Christopher McFarland, Keith R. Loeb, William J. Valente, Nolan G. Ericson, Emily A. Stevens, Jerald P. Radich, Tarjei S. Mikkelsen, Benjamin J. Hindson, Jason H. Bielas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/9c33171e807745c7908a1e1e9a3712e3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9c33171e807745c7908a1e1e9a3712e3
record_format	dspace
spelling	oai:doaj.org-article:9c33171e807745c7908a1e1e9a3712e32021-12-02T15:38:46ZMassively parallel digital transcriptional profiling of single cells10.1038/ncomms140492041-1723https://doaj.org/article/9c33171e807745c7908a1e1e9a3712e32017-01-01T00:00:00Zhttps://doi.org/10.1038/ncomms14049https://doaj.org/toc/2041-1723Single-cell gene expression analysis is challenging. This work describes a new droplet-based single cell RNA-seq platform capable of processing tens of thousands of cells across 8 independent samples in minutes, and demonstrates cellular subtypes and host–donor chimerism in transplant patients.Grace X. Y. ZhengJessica M. TerryPhillip BelgraderPaul RyvkinZachary W. BentRyan WilsonSolongo B. ZiraldoTobias D. WheelerGeoff P. McDermottJunjie ZhuMark T. GregoryJoe ShugaLuz MontesclarosJason G. UnderwoodDonald A. MasquelierStefanie Y. NishimuraMichael Schnall-LevinPaul W. WyattChristopher M. HindsonRajiv BharadwajAlexander WongKevin D. NessLan W. BeppuH. Joachim DeegChristopher McFarlandKeith R. LoebWilliam J. ValenteNolan G. EricsonEmily A. StevensJerald P. RadichTarjei S. MikkelsenBenjamin J. HindsonJason H. BielasNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Grace X. Y. Zheng Jessica M. Terry Phillip Belgrader Paul Ryvkin Zachary W. Bent Ryan Wilson Solongo B. Ziraldo Tobias D. Wheeler Geoff P. McDermott Junjie Zhu Mark T. Gregory Joe Shuga Luz Montesclaros Jason G. Underwood Donald A. Masquelier Stefanie Y. Nishimura Michael Schnall-Levin Paul W. Wyatt Christopher M. Hindson Rajiv Bharadwaj Alexander Wong Kevin D. Ness Lan W. Beppu H. Joachim Deeg Christopher McFarland Keith R. Loeb William J. Valente Nolan G. Ericson Emily A. Stevens Jerald P. Radich Tarjei S. Mikkelsen Benjamin J. Hindson Jason H. Bielas Massively parallel digital transcriptional profiling of single cells
description	Single-cell gene expression analysis is challenging. This work describes a new droplet-based single cell RNA-seq platform capable of processing tens of thousands of cells across 8 independent samples in minutes, and demonstrates cellular subtypes and host–donor chimerism in transplant patients.
format	article
author	Grace X. Y. Zheng Jessica M. Terry Phillip Belgrader Paul Ryvkin Zachary W. Bent Ryan Wilson Solongo B. Ziraldo Tobias D. Wheeler Geoff P. McDermott Junjie Zhu Mark T. Gregory Joe Shuga Luz Montesclaros Jason G. Underwood Donald A. Masquelier Stefanie Y. Nishimura Michael Schnall-Levin Paul W. Wyatt Christopher M. Hindson Rajiv Bharadwaj Alexander Wong Kevin D. Ness Lan W. Beppu H. Joachim Deeg Christopher McFarland Keith R. Loeb William J. Valente Nolan G. Ericson Emily A. Stevens Jerald P. Radich Tarjei S. Mikkelsen Benjamin J. Hindson Jason H. Bielas
author_facet	Grace X. Y. Zheng Jessica M. Terry Phillip Belgrader Paul Ryvkin Zachary W. Bent Ryan Wilson Solongo B. Ziraldo Tobias D. Wheeler Geoff P. McDermott Junjie Zhu Mark T. Gregory Joe Shuga Luz Montesclaros Jason G. Underwood Donald A. Masquelier Stefanie Y. Nishimura Michael Schnall-Levin Paul W. Wyatt Christopher M. Hindson Rajiv Bharadwaj Alexander Wong Kevin D. Ness Lan W. Beppu H. Joachim Deeg Christopher McFarland Keith R. Loeb William J. Valente Nolan G. Ericson Emily A. Stevens Jerald P. Radich Tarjei S. Mikkelsen Benjamin J. Hindson Jason H. Bielas
author_sort	Grace X. Y. Zheng
title	Massively parallel digital transcriptional profiling of single cells
title_short	Massively parallel digital transcriptional profiling of single cells
title_full	Massively parallel digital transcriptional profiling of single cells
title_fullStr	Massively parallel digital transcriptional profiling of single cells
title_full_unstemmed	Massively parallel digital transcriptional profiling of single cells
title_sort	massively parallel digital transcriptional profiling of single cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9c33171e807745c7908a1e1e9a3712e3
work_keys_str_mv	AT gracexyzheng massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT jessicamterry massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT phillipbelgrader massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT paulryvkin massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT zacharywbent massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT ryanwilson massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT solongobziraldo massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT tobiasdwheeler massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT geoffpmcdermott massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT junjiezhu massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT marktgregory massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT joeshuga massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT luzmontesclaros massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT jasongunderwood massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT donaldamasquelier massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT stefanieynishimura massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT michaelschnalllevin massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT paulwwyatt massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT christophermhindson massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT rajivbharadwaj massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT alexanderwong massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT kevindness massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT lanwbeppu massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT hjoachimdeeg massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT christophermcfarland massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT keithrloeb massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT williamjvalente massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT nolangericson massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT emilyastevens massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT jeraldpradich massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT tarjeismikkelsen massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT benjaminjhindson massivelyparalleldigitaltranscriptionalprofilingofsinglecells AT jasonhbielas massivelyparalleldigitaltranscriptionalprofilingofsinglecells
_version_	1718386129452924928

Massively parallel digital transcriptional profiling of single cells

Ejemplares similares