Analysis of mitochondrial function and localisation during human embryonic stem cell differentiation in vitro.

Human embryonic stem cell (hESC) derivatives show promise as viable cell therapy options for multiple disorders in different tissues. Recent advances in stem cell biology have lead to the reliable production and detailed molecular characterisation of a range of cell-types. However, the role of mitoc...

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Auteurs principaux:	Andrew B J Prowse, Fenny Chong, David A Elliott, Andrew G Elefanty, Edouard G Stanley, Peter P Gray, Trent P Munro, Geoffrey W Osborne
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2012
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/9c33bcc235e34987917f4b0979d649ed
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spelling	oai:doaj.org-article:9c33bcc235e34987917f4b0979d649ed2021-11-18T08:04:28ZAnalysis of mitochondrial function and localisation during human embryonic stem cell differentiation in vitro.1932-620310.1371/journal.pone.0052214https://doaj.org/article/9c33bcc235e34987917f4b0979d649ed2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23284940/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Human embryonic stem cell (hESC) derivatives show promise as viable cell therapy options for multiple disorders in different tissues. Recent advances in stem cell biology have lead to the reliable production and detailed molecular characterisation of a range of cell-types. However, the role of mitochondria during differentiation has yet to be fully elucidated. Mitochondria mediate a cells response to altered energy requirements (e.g. cardiomyocyte contraction) and, as such, the mitochondrial phenotype is likely to change during the dynamic process of hESC differentiation. We demonstrate that manipulating mitochondrial biogenesis alters mesendoderm commitment. To investigate mitochondrial localisation during early lineage specification of hESCs we developed a mitochondrial reporter line, KMEL2, in which sequences encoding the green fluorescent protein (GFP) are targeted to the mitochondria. Differentiation of KMEL2 lines into the three germ layers showed that the mitochondria in these differentiated progeny are GFP positive. Therefore, KMEL2 hESCs facilitate the study of mitochondria in a range of cell types and, importantly, permit real-time analysis of mitochondria via the GFP tag.Andrew B J ProwseFenny ChongDavid A ElliottAndrew G ElefantyEdouard G StanleyPeter P GrayTrent P MunroGeoffrey W OsbornePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52214 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrew B J Prowse Fenny Chong David A Elliott Andrew G Elefanty Edouard G Stanley Peter P Gray Trent P Munro Geoffrey W Osborne Analysis of mitochondrial function and localisation during human embryonic stem cell differentiation in vitro.
description	Human embryonic stem cell (hESC) derivatives show promise as viable cell therapy options for multiple disorders in different tissues. Recent advances in stem cell biology have lead to the reliable production and detailed molecular characterisation of a range of cell-types. However, the role of mitochondria during differentiation has yet to be fully elucidated. Mitochondria mediate a cells response to altered energy requirements (e.g. cardiomyocyte contraction) and, as such, the mitochondrial phenotype is likely to change during the dynamic process of hESC differentiation. We demonstrate that manipulating mitochondrial biogenesis alters mesendoderm commitment. To investigate mitochondrial localisation during early lineage specification of hESCs we developed a mitochondrial reporter line, KMEL2, in which sequences encoding the green fluorescent protein (GFP) are targeted to the mitochondria. Differentiation of KMEL2 lines into the three germ layers showed that the mitochondria in these differentiated progeny are GFP positive. Therefore, KMEL2 hESCs facilitate the study of mitochondria in a range of cell types and, importantly, permit real-time analysis of mitochondria via the GFP tag.
format	article
author	Andrew B J Prowse Fenny Chong David A Elliott Andrew G Elefanty Edouard G Stanley Peter P Gray Trent P Munro Geoffrey W Osborne
author_facet	Andrew B J Prowse Fenny Chong David A Elliott Andrew G Elefanty Edouard G Stanley Peter P Gray Trent P Munro Geoffrey W Osborne
author_sort	Andrew B J Prowse
title	Analysis of mitochondrial function and localisation during human embryonic stem cell differentiation in vitro.
title_short	Analysis of mitochondrial function and localisation during human embryonic stem cell differentiation in vitro.
title_full	Analysis of mitochondrial function and localisation during human embryonic stem cell differentiation in vitro.
title_fullStr	Analysis of mitochondrial function and localisation during human embryonic stem cell differentiation in vitro.
title_full_unstemmed	Analysis of mitochondrial function and localisation during human embryonic stem cell differentiation in vitro.
title_sort	analysis of mitochondrial function and localisation during human embryonic stem cell differentiation in vitro.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/9c33bcc235e34987917f4b0979d649ed
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Analysis of mitochondrial function and localisation during human embryonic stem cell differentiation in vitro.

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