Synthesized zinc peroxide nanoparticles (ZnO2-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds

Sameh Samir Ali,1,2,* Reda Morsy,3,4,* Nessma Ahmed El-Zawawy,2 Mervat F Fareed,5,6 Mohamed Yaser Bedaiwy2 1Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, China; 2Botany Department, Faculty of Science, Tanta University, Tanta, Egypt; 3Physics De...

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Autores principales:	Ali SS, Morsy R, El-Zawawy NA, Fareed MF, Bedaiwy MY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Nanomaterial Multidrug resistance Pseudomonas aeruginosa Aspergillus niger Skin burns Histopathology. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9c432d4e2f9f4de497dfab29bef565db
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spelling	oai:doaj.org-article:9c432d4e2f9f4de497dfab29bef565db2021-12-02T07:46:33ZSynthesized zinc peroxide nanoparticles (ZnO2-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds1178-2013https://doaj.org/article/9c432d4e2f9f4de497dfab29bef565db2017-08-01T00:00:00Zhttps://www.dovepress.com/synthesized-zinc-peroxide-nanoparticles-zno2-nps-a-novel-antimicrobial-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sameh Samir Ali,1,2,* Reda Morsy,3,4,* Nessma Ahmed El-Zawawy,2 Mervat F Fareed,5,6 Mohamed Yaser Bedaiwy2 1Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, China; 2Botany Department, Faculty of Science, Tanta University, Tanta, Egypt; 3Physics Department, Faculty of Science, Tanta University, Tanta, Egypt; 4Physics Department, Faculty of Dentistry, Al Baha University, Al Baha, Saudi Arabia; 5Department of Home Economic, Faculty of Specific Education, Tanta University, Tanta, Egypt; 6Department of Biology, Faculty of Science, Taif University, Taif, Saudi Arabia *These authors contributed equally to this work Abstract: Increasing of multidrug resistance (MDR) remains an intractable challenge for burn patients. Innovative nanomaterials are also in high demand for the development of new antimicrobial biomaterials that inevitably have opened new therapeutic horizons in medical approaches and lead to many efforts for synthesizing new metal oxide nanoparticles (NPs) for better control of the MDR associated with the polymicrobial burn wounds. Recently, it seems that metal oxides can truly be considered as highly efficient inorganic agents with antimicrobial properties. In this study, zinc peroxide NPs (ZnO2-NPs) were synthesized using the co-precipitation method. Synthesized ZnO2-NPs were characterized by X-ray diffraction, Fourier transformed infrared, transmission electron microscopy, thermogravimetric analysis, differential scanning calorimetry, and ultraviolet-visible spectroscopy. The characterization techniques revealed synthesis of the pure phase of non-agglomerated ZnO2-NPs having sizes in the range of 15–25 nm with a transition temperature of 211°C. Antimicrobial activity of ZnO2-NPs was determined against MDR Pseudomonas aeruginosa (PA) and Aspergillus niger (AN) strains isolated from burn wound infections. Both strains, PA6 and AN4, were found to be more susceptible strains to ZnO2-NPs. In addition, a significant decrease in elastase and keratinase activities was recorded with increased concentrations of ZnO2-NPs until 200 µg/mL. ZnO2-NPs revealed a significant anti-inflammatory activity against PA6 and AN4 strains as demonstrated by membrane stabilization, albumin denaturation, and proteinase inhibition. Moreover, the results of in vivo histopathology assessment confirmed the potential role of ZnO2-NPs in the improvement of skin wound healing in the experimental animal models. Clearly, the synthesized ZnO2-NPs have demonstrated a competitive capability as antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory candidates, suggesting that the ZnO2-NPs are promising metal oxides that are potentially valued for biomedical applications. Keywords: co-precipitation method, burn wound infections, metal oxides nanoparticles, multidrug resistance, antimicrobial, anti-inflammatoryAli SSMorsy REl-Zawawy NAFareed MFBedaiwy MYDove Medical PressarticleNanomaterialMultidrug resistancePseudomonas aeruginosaAspergillus nigerSkin burnsHistopathology.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 6059-6073 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanomaterial Multidrug resistance Pseudomonas aeruginosa Aspergillus niger Skin burns Histopathology. Medicine (General) R5-920
spellingShingle	Nanomaterial Multidrug resistance Pseudomonas aeruginosa Aspergillus niger Skin burns Histopathology. Medicine (General) R5-920 Ali SS Morsy R El-Zawawy NA Fareed MF Bedaiwy MY Synthesized zinc peroxide nanoparticles (ZnO2-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds
description	Sameh Samir Ali,1,2,* Reda Morsy,3,4,* Nessma Ahmed El-Zawawy,2 Mervat F Fareed,5,6 Mohamed Yaser Bedaiwy2 1Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, China; 2Botany Department, Faculty of Science, Tanta University, Tanta, Egypt; 3Physics Department, Faculty of Science, Tanta University, Tanta, Egypt; 4Physics Department, Faculty of Dentistry, Al Baha University, Al Baha, Saudi Arabia; 5Department of Home Economic, Faculty of Specific Education, Tanta University, Tanta, Egypt; 6Department of Biology, Faculty of Science, Taif University, Taif, Saudi Arabia *These authors contributed equally to this work Abstract: Increasing of multidrug resistance (MDR) remains an intractable challenge for burn patients. Innovative nanomaterials are also in high demand for the development of new antimicrobial biomaterials that inevitably have opened new therapeutic horizons in medical approaches and lead to many efforts for synthesizing new metal oxide nanoparticles (NPs) for better control of the MDR associated with the polymicrobial burn wounds. Recently, it seems that metal oxides can truly be considered as highly efficient inorganic agents with antimicrobial properties. In this study, zinc peroxide NPs (ZnO2-NPs) were synthesized using the co-precipitation method. Synthesized ZnO2-NPs were characterized by X-ray diffraction, Fourier transformed infrared, transmission electron microscopy, thermogravimetric analysis, differential scanning calorimetry, and ultraviolet-visible spectroscopy. The characterization techniques revealed synthesis of the pure phase of non-agglomerated ZnO2-NPs having sizes in the range of 15–25 nm with a transition temperature of 211°C. Antimicrobial activity of ZnO2-NPs was determined against MDR Pseudomonas aeruginosa (PA) and Aspergillus niger (AN) strains isolated from burn wound infections. Both strains, PA6 and AN4, were found to be more susceptible strains to ZnO2-NPs. In addition, a significant decrease in elastase and keratinase activities was recorded with increased concentrations of ZnO2-NPs until 200 µg/mL. ZnO2-NPs revealed a significant anti-inflammatory activity against PA6 and AN4 strains as demonstrated by membrane stabilization, albumin denaturation, and proteinase inhibition. Moreover, the results of in vivo histopathology assessment confirmed the potential role of ZnO2-NPs in the improvement of skin wound healing in the experimental animal models. Clearly, the synthesized ZnO2-NPs have demonstrated a competitive capability as antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory candidates, suggesting that the ZnO2-NPs are promising metal oxides that are potentially valued for biomedical applications. Keywords: co-precipitation method, burn wound infections, metal oxides nanoparticles, multidrug resistance, antimicrobial, anti-inflammatory
format	article
author	Ali SS Morsy R El-Zawawy NA Fareed MF Bedaiwy MY
author_facet	Ali SS Morsy R El-Zawawy NA Fareed MF Bedaiwy MY
author_sort	Ali SS
title	Synthesized zinc peroxide nanoparticles (ZnO2-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds
title_short	Synthesized zinc peroxide nanoparticles (ZnO2-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds
title_full	Synthesized zinc peroxide nanoparticles (ZnO2-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds
title_fullStr	Synthesized zinc peroxide nanoparticles (ZnO2-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds
title_full_unstemmed	Synthesized zinc peroxide nanoparticles (ZnO2-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds
title_sort	synthesized zinc peroxide nanoparticles (zno2-nps): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/9c432d4e2f9f4de497dfab29bef565db
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Synthesized zinc peroxide nanoparticles (ZnO2-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds

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