Antiangiogenesis Activity Study of Awar-Awar Leaf Ethanolic Extract (Ficus Septica Burm. F.) By Chick Chorioallantoic Membrane Method

Cancer is one of the biggest causes of death after cardiovascular disease. Furthermore, alkaloid content of awar-awar leaf extract (Ficus septica Burm. f.) is reported to exhibit cytotoxic activity in several cancer cells. This research aims to identify the antiangiogenesis activity and potential of...

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Autores principales:	Lailiana Garna Nurhidayati, Agung Endro Nugroho, Bambang Retnoaji, Sudarsono Sudarsono, Nanang Fakhrudin
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	angiogenesis awar-awar leaf ethanol extract ficus septica burm. f. Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/9c45b49c25bc42c0b9f3a03eb224ade6
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spelling	oai:doaj.org-article:9c45b49c25bc42c0b9f3a03eb224ade62021-11-15T06:08:47ZAntiangiogenesis Activity Study of Awar-Awar Leaf Ethanolic Extract (Ficus Septica Burm. F.) By Chick Chorioallantoic Membrane Method2338-94272338-948610.22146/ijp.607https://doaj.org/article/9c45b49c25bc42c0b9f3a03eb224ade62021-01-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/607https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Cancer is one of the biggest causes of death after cardiovascular disease. Furthermore, alkaloid content of awar-awar leaf extract (Ficus septica Burm. f.) is reported to exhibit cytotoxic activity in several cancer cells. This research aims to identify the antiangiogenesis activity and potential of awar-awar leaf extract (Ficus septica Burm. f.) on chorioallantoic membrane (CAM) of chicken embryo inducted with basic fibroblast growth factor (bFGF). Awar-awar leaf extract was obtained by the maceration method by using ethanol 70%. CAM was cultured in ex ovo and then angiogenesis induced by bFGF and treated by using the concentrated extract of 0.013; 0.00975; 0.0065; and 0.00325 mg/mL. From now on, the observation was done macroscopically and microscopically. Macroscopic observation result shows that an inhibition occurred at the concentration extract of 0.013 mg/mL or equal to 62.30%, 0.00975 mg/mL or equal to 51.37%, 0.0065 mg/mL or equal to 28.42%, and 0.00325 mg/mL or equal to 13.11%. Meanwhile, the microscopic observation result shows that the higher concentration of the extract, the growth of new blood vessels is decreased, demonstrated by the reduced erythrocyte density, parallel blood vessel pattern, and looser cellular density mesenchymal tissue. Moreover, the statistical analysis result shows that the increasing concentration of extract could increase the inhibition of new blood vessel growth (p <0.05). So, awar-awar leaf ethanol extract can be used as an antiangiogenesis agent in CAM of chicken embryo induced with bFGF by ex ovo.Lailiana Garna NurhidayatiAgung Endro NugrohoBambang RetnoajiSudarsono SudarsonoNanang FakhrudinUniversitas Gadjah Madaarticleangiogenesisawar-awar leaf ethanol extractficus septica burm. f.Pharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	angiogenesis awar-awar leaf ethanol extract ficus septica burm. f. Pharmacy and materia medica RS1-441
spellingShingle	angiogenesis awar-awar leaf ethanol extract ficus septica burm. f. Pharmacy and materia medica RS1-441 Lailiana Garna Nurhidayati Agung Endro Nugroho Bambang Retnoaji Sudarsono Sudarsono Nanang Fakhrudin Antiangiogenesis Activity Study of Awar-Awar Leaf Ethanolic Extract (Ficus Septica Burm. F.) By Chick Chorioallantoic Membrane Method
description	Cancer is one of the biggest causes of death after cardiovascular disease. Furthermore, alkaloid content of awar-awar leaf extract (Ficus septica Burm. f.) is reported to exhibit cytotoxic activity in several cancer cells. This research aims to identify the antiangiogenesis activity and potential of awar-awar leaf extract (Ficus septica Burm. f.) on chorioallantoic membrane (CAM) of chicken embryo inducted with basic fibroblast growth factor (bFGF). Awar-awar leaf extract was obtained by the maceration method by using ethanol 70%. CAM was cultured in ex ovo and then angiogenesis induced by bFGF and treated by using the concentrated extract of 0.013; 0.00975; 0.0065; and 0.00325 mg/mL. From now on, the observation was done macroscopically and microscopically. Macroscopic observation result shows that an inhibition occurred at the concentration extract of 0.013 mg/mL or equal to 62.30%, 0.00975 mg/mL or equal to 51.37%, 0.0065 mg/mL or equal to 28.42%, and 0.00325 mg/mL or equal to 13.11%. Meanwhile, the microscopic observation result shows that the higher concentration of the extract, the growth of new blood vessels is decreased, demonstrated by the reduced erythrocyte density, parallel blood vessel pattern, and looser cellular density mesenchymal tissue. Moreover, the statistical analysis result shows that the increasing concentration of extract could increase the inhibition of new blood vessel growth (p <0.05). So, awar-awar leaf ethanol extract can be used as an antiangiogenesis agent in CAM of chicken embryo induced with bFGF by ex ovo.
format	article
author	Lailiana Garna Nurhidayati Agung Endro Nugroho Bambang Retnoaji Sudarsono Sudarsono Nanang Fakhrudin
author_facet	Lailiana Garna Nurhidayati Agung Endro Nugroho Bambang Retnoaji Sudarsono Sudarsono Nanang Fakhrudin
author_sort	Lailiana Garna Nurhidayati
title	Antiangiogenesis Activity Study of Awar-Awar Leaf Ethanolic Extract (Ficus Septica Burm. F.) By Chick Chorioallantoic Membrane Method
title_short	Antiangiogenesis Activity Study of Awar-Awar Leaf Ethanolic Extract (Ficus Septica Burm. F.) By Chick Chorioallantoic Membrane Method
title_full	Antiangiogenesis Activity Study of Awar-Awar Leaf Ethanolic Extract (Ficus Septica Burm. F.) By Chick Chorioallantoic Membrane Method
title_fullStr	Antiangiogenesis Activity Study of Awar-Awar Leaf Ethanolic Extract (Ficus Septica Burm. F.) By Chick Chorioallantoic Membrane Method
title_full_unstemmed	Antiangiogenesis Activity Study of Awar-Awar Leaf Ethanolic Extract (Ficus Septica Burm. F.) By Chick Chorioallantoic Membrane Method
title_sort	antiangiogenesis activity study of awar-awar leaf ethanolic extract (ficus septica burm. f.) by chick chorioallantoic membrane method
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/9c45b49c25bc42c0b9f3a03eb224ade6
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Antiangiogenesis Activity Study of Awar-Awar Leaf Ethanolic Extract (Ficus Septica Burm. F.) By Chick Chorioallantoic Membrane Method

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