Rhizospheric bacteria as potential biocontrol agents against Fusarium wilt and crown and root rot diseases in tomato

The discovery of novel biocontrol agents requires the continuous scrutiny of native microorganisms to ensure that they will be useful on a regional scale. The goal of the present work was to discover novel antagonistic bacteria against Fusarium oxysporum ff. spp. lycopersici race 3 (Fol R3) and radi...

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Autores principales:	Md. Masudur Rahman Khalil, Rosario Alicia Fierro-Coronado, Ofelda Peñuelas-Rubio, Alma Guadalupe Villa-Lerma, Rigoberto Plascencia-Jatomea, Rubén Félix-Gastélum, Ignacio Eduardo Maldonado-Mendoza
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Acinetobacter Antagonist Bacillus Plant-growth promotion Tomato hybrid specificity Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9c4b634fa9f64ee1a1515e46ef2cff72
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spelling	oai:doaj.org-article:9c4b634fa9f64ee1a1515e46ef2cff722021-11-20T04:57:05ZRhizospheric bacteria as potential biocontrol agents against Fusarium wilt and crown and root rot diseases in tomato1319-562X10.1016/j.sjbs.2021.08.043https://doaj.org/article/9c4b634fa9f64ee1a1515e46ef2cff722021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21007312https://doaj.org/toc/1319-562XThe discovery of novel biocontrol agents requires the continuous scrutiny of native microorganisms to ensure that they will be useful on a regional scale. The goal of the present work was to discover novel antagonistic bacteria against Fusarium oxysporum ff. spp. lycopersici race 3 (Fol R3) and radicis-lycopersici (Forl) causing Fusarium wilt disease and Fusarium crown and root rot of tomatoes, respectively. High-throughput liquid antagonism screening of 1,875 rhizospheric bacterial strains followed by dual confrontation assays in 96-well plates was used to select bacteria exhibiting > 50% fungal growth inhibition. In a second dual confrontation assay in 10-cm Petri dishes, bacteria showing > 20% Fol R3 or Forl growth inhibition were further screened using a blood hemolysis test. After discarding β-hemolytic bacteria, a seedling antagonistic assay was performed to select five potential antagonists. A phylogenetic analysis of 16S rRNA identified one strain as Acinetobacter calcoaceticus (AcDB3) and four strains as members of the genus Bacillus (B. amyloliquefaciens BaMA26, Bacillus siamensis BsiDA2, B. subtilis BsTA16 and B. thuringiensis BtMB9). Greenhouse assays demonstrated that BsTA16 and AcDB3 were the most promising antagonists against Fol R3 and Forl, respectively. Pathogen biocontrol and growth promotion mechanisms used by these bacteria include the production of siderophores, biofilm, proteases, endoglucanases and indole acetic acid, and phosphate solubilization. These five bacteria exerted differential responses on pathogen control depending on the tomato hybrid, and on the growth stage of tomatoes. We report for the first time the use of an Acinetobacter calcoaceticus isolate (AcDB3) to control Forl in tomato under greenhouse conditions.Md. Masudur Rahman KhalilRosario Alicia Fierro-CoronadoOfelda Peñuelas-RubioAlma Guadalupe Villa-LermaRigoberto Plascencia-JatomeaRubén Félix-GastélumIgnacio Eduardo Maldonado-MendozaElsevierarticleAcinetobacterAntagonistBacillusPlant-growth promotionTomato hybrid specificityBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 7460-7471 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Acinetobacter Antagonist Bacillus Plant-growth promotion Tomato hybrid specificity Biology (General) QH301-705.5
spellingShingle	Acinetobacter Antagonist Bacillus Plant-growth promotion Tomato hybrid specificity Biology (General) QH301-705.5 Md. Masudur Rahman Khalil Rosario Alicia Fierro-Coronado Ofelda Peñuelas-Rubio Alma Guadalupe Villa-Lerma Rigoberto Plascencia-Jatomea Rubén Félix-Gastélum Ignacio Eduardo Maldonado-Mendoza Rhizospheric bacteria as potential biocontrol agents against Fusarium wilt and crown and root rot diseases in tomato
description	The discovery of novel biocontrol agents requires the continuous scrutiny of native microorganisms to ensure that they will be useful on a regional scale. The goal of the present work was to discover novel antagonistic bacteria against Fusarium oxysporum ff. spp. lycopersici race 3 (Fol R3) and radicis-lycopersici (Forl) causing Fusarium wilt disease and Fusarium crown and root rot of tomatoes, respectively. High-throughput liquid antagonism screening of 1,875 rhizospheric bacterial strains followed by dual confrontation assays in 96-well plates was used to select bacteria exhibiting > 50% fungal growth inhibition. In a second dual confrontation assay in 10-cm Petri dishes, bacteria showing > 20% Fol R3 or Forl growth inhibition were further screened using a blood hemolysis test. After discarding β-hemolytic bacteria, a seedling antagonistic assay was performed to select five potential antagonists. A phylogenetic analysis of 16S rRNA identified one strain as Acinetobacter calcoaceticus (AcDB3) and four strains as members of the genus Bacillus (B. amyloliquefaciens BaMA26, Bacillus siamensis BsiDA2, B. subtilis BsTA16 and B. thuringiensis BtMB9). Greenhouse assays demonstrated that BsTA16 and AcDB3 were the most promising antagonists against Fol R3 and Forl, respectively. Pathogen biocontrol and growth promotion mechanisms used by these bacteria include the production of siderophores, biofilm, proteases, endoglucanases and indole acetic acid, and phosphate solubilization. These five bacteria exerted differential responses on pathogen control depending on the tomato hybrid, and on the growth stage of tomatoes. We report for the first time the use of an Acinetobacter calcoaceticus isolate (AcDB3) to control Forl in tomato under greenhouse conditions.
format	article
author	Md. Masudur Rahman Khalil Rosario Alicia Fierro-Coronado Ofelda Peñuelas-Rubio Alma Guadalupe Villa-Lerma Rigoberto Plascencia-Jatomea Rubén Félix-Gastélum Ignacio Eduardo Maldonado-Mendoza
author_facet	Md. Masudur Rahman Khalil Rosario Alicia Fierro-Coronado Ofelda Peñuelas-Rubio Alma Guadalupe Villa-Lerma Rigoberto Plascencia-Jatomea Rubén Félix-Gastélum Ignacio Eduardo Maldonado-Mendoza
author_sort	Md. Masudur Rahman Khalil
title	Rhizospheric bacteria as potential biocontrol agents against Fusarium wilt and crown and root rot diseases in tomato
title_short	Rhizospheric bacteria as potential biocontrol agents against Fusarium wilt and crown and root rot diseases in tomato
title_full	Rhizospheric bacteria as potential biocontrol agents against Fusarium wilt and crown and root rot diseases in tomato
title_fullStr	Rhizospheric bacteria as potential biocontrol agents against Fusarium wilt and crown and root rot diseases in tomato
title_full_unstemmed	Rhizospheric bacteria as potential biocontrol agents against Fusarium wilt and crown and root rot diseases in tomato
title_sort	rhizospheric bacteria as potential biocontrol agents against fusarium wilt and crown and root rot diseases in tomato
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/9c4b634fa9f64ee1a1515e46ef2cff72
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Rhizospheric bacteria as potential biocontrol agents against Fusarium wilt and crown and root rot diseases in tomato

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