Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes

Background: Oocyte quality and maturation are influenced by protein supplementation. Objective: To evaluate the influence of fetal bovine serum (FBS) and bovine serum albumin (BSA) concentrations on the recovery and in vitro maturation (IVM) of bovine oocytes. Methods: The study was divided into Sta...

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Autores principales:	Pedro H. Fernandes-França, Maria V. Oliveira-Santos, Gabriela P. Oliveira-Lira, Alana Azevedo-Borges, Alexsandra Fernandes-Pereira
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2019
Materias:	bovine serum albumin brilliant cresyl blue fetal bovine serum in vitro embryo production meiotic competence oocyte maturation oocyte quality oocyte recovery Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/9c4fa451baf7402d9a0c23639252c804
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spelling	oai:doaj.org-article:9c4fa451baf7402d9a0c23639252c8042021-12-01T15:27:54ZInfluence of different protein supplements on the recovery and in vitro maturation of bovine oocytes2256-295810.17533/udea.rccp.v33n3a01https://doaj.org/article/9c4fa451baf7402d9a0c23639252c8042019-12-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/340677https://doaj.org/toc/2256-2958Background: Oocyte quality and maturation are influenced by protein supplementation. Objective: To evaluate the influence of fetal bovine serum (FBS) and bovine serum albumin (BSA) concentrations on the recovery and in vitro maturation (IVM) of bovine oocytes. Methods: The study was divided into Stage 1 (oocyte recovery), and Stage 2 (IVM). In the first stage, three experiments were conducted according to the recovery (R) medium used: (R1) 10 vs. 20% FBS; (R2) 5 vs. 10% BSA; and (R3) the best results from R1, R2, and the combination of FBS+BSA (5+5%). Within the second stage, the maturation medium was supplemented according to three experiments: (M1) 5 vs. 10% FBS; (M2) 0.4 vs. 0.8% BSA; and (M3) better results of M1, M2, and the combination of FBS+BSA (5+0.8%). Results: In Stage 1 (R1 and R2), the media with 10% FBS and 10% BSA showed better oocyte quality results and were defined for experiment R3. In R3, the 10% FBS and the combination of FBS+BSA (5+5%) allowed recovery of better-quality oocytes. In Stage 2 (M1 and M2), media with both levels of FBS (5 and 10%) and 0.8% BSA were defined as better according to the maturation and viability rates of cumulus cells, so they were defined for experiment M3. In M3, no difference was noted among the supplements. Conclusions: For oocyte recovery, 10% FBS and the combination of FBS+BSA (5+5%) can be used to obtain immature oocytes. For the in vitro maturation, FBS (both levels, 5 and 10%) and BSA (0.8%) can be used alone or in combination.Pedro H. Fernandes-FrançaMaria V. Oliveira-SantosGabriela P. Oliveira-LiraAlana Azevedo-BorgesAlexsandra Fernandes-PereiraUniversidad de Antioquiaarticlebovine serum albuminbrilliant cresyl bluefetal bovine serumin vitro embryo productionmeiotic competenceoocyte maturationoocyte qualityoocyte recoveryAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 33, Iss 3, Pp 172-181 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	bovine serum albumin brilliant cresyl blue fetal bovine serum in vitro embryo production meiotic competence oocyte maturation oocyte quality oocyte recovery Animal culture SF1-1100
spellingShingle	bovine serum albumin brilliant cresyl blue fetal bovine serum in vitro embryo production meiotic competence oocyte maturation oocyte quality oocyte recovery Animal culture SF1-1100 Pedro H. Fernandes-França Maria V. Oliveira-Santos Gabriela P. Oliveira-Lira Alana Azevedo-Borges Alexsandra Fernandes-Pereira Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes
description	Background: Oocyte quality and maturation are influenced by protein supplementation. Objective: To evaluate the influence of fetal bovine serum (FBS) and bovine serum albumin (BSA) concentrations on the recovery and in vitro maturation (IVM) of bovine oocytes. Methods: The study was divided into Stage 1 (oocyte recovery), and Stage 2 (IVM). In the first stage, three experiments were conducted according to the recovery (R) medium used: (R1) 10 vs. 20% FBS; (R2) 5 vs. 10% BSA; and (R3) the best results from R1, R2, and the combination of FBS+BSA (5+5%). Within the second stage, the maturation medium was supplemented according to three experiments: (M1) 5 vs. 10% FBS; (M2) 0.4 vs. 0.8% BSA; and (M3) better results of M1, M2, and the combination of FBS+BSA (5+0.8%). Results: In Stage 1 (R1 and R2), the media with 10% FBS and 10% BSA showed better oocyte quality results and were defined for experiment R3. In R3, the 10% FBS and the combination of FBS+BSA (5+5%) allowed recovery of better-quality oocytes. In Stage 2 (M1 and M2), media with both levels of FBS (5 and 10%) and 0.8% BSA were defined as better according to the maturation and viability rates of cumulus cells, so they were defined for experiment M3. In M3, no difference was noted among the supplements. Conclusions: For oocyte recovery, 10% FBS and the combination of FBS+BSA (5+5%) can be used to obtain immature oocytes. For the in vitro maturation, FBS (both levels, 5 and 10%) and BSA (0.8%) can be used alone or in combination.
format	article
author	Pedro H. Fernandes-França Maria V. Oliveira-Santos Gabriela P. Oliveira-Lira Alana Azevedo-Borges Alexsandra Fernandes-Pereira
author_facet	Pedro H. Fernandes-França Maria V. Oliveira-Santos Gabriela P. Oliveira-Lira Alana Azevedo-Borges Alexsandra Fernandes-Pereira
author_sort	Pedro H. Fernandes-França
title	Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes
title_short	Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes
title_full	Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes
title_fullStr	Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes
title_full_unstemmed	Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes
title_sort	influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes
publisher	Universidad de Antioquia
publishDate	2019
url	https://doaj.org/article/9c4fa451baf7402d9a0c23639252c804
work_keys_str_mv	AT pedrohfernandesfranca influenceofdifferentproteinsupplementsontherecoveryandinvitromaturationofbovineoocytes AT mariavoliveirasantos influenceofdifferentproteinsupplementsontherecoveryandinvitromaturationofbovineoocytes AT gabrielapoliveiralira influenceofdifferentproteinsupplementsontherecoveryandinvitromaturationofbovineoocytes AT alanaazevedoborges influenceofdifferentproteinsupplementsontherecoveryandinvitromaturationofbovineoocytes AT alexsandrafernandespereira influenceofdifferentproteinsupplementsontherecoveryandinvitromaturationofbovineoocytes
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Influence of different protein supplements on the recovery and in vitro maturation of bovine oocytes

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