The innate immune response elicited by Group A Streptococcus is highly variable among clinical isolates and correlates with the emm type.

Group A Streptococcus (GAS) infections remain a significant health care problem due to high morbidity and mortality associated with GAS diseases, along with their increasing worldwide prevalence. Macrophages play a key role in the control and clearance of GAS infections. Moreover, pro-inflammatory c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Márcia Dinis, Céline Plainvert, Pavel Kovarik, Magalie Longo, Agnès Fouet, Claire Poyart
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9c5cae4d8391428c8bc358d079e76120
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9c5cae4d8391428c8bc358d079e76120
record_format	dspace
spelling	oai:doaj.org-article:9c5cae4d8391428c8bc358d079e761202021-11-25T06:09:47ZThe innate immune response elicited by Group A Streptococcus is highly variable among clinical isolates and correlates with the emm type.1932-620310.1371/journal.pone.0101464https://doaj.org/article/9c5cae4d8391428c8bc358d079e761202014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24991887/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Group A Streptococcus (GAS) infections remain a significant health care problem due to high morbidity and mortality associated with GAS diseases, along with their increasing worldwide prevalence. Macrophages play a key role in the control and clearance of GAS infections. Moreover, pro-inflammatory cytokines production and GAS persistence and invasion are related. In this study we investigated the correlation between the GAS clinical isolates genotypes, their known clinical history, and their ability to modulate innate immune response. We constituted a collection of 40 independent GAS isolates representative of the emm types currently prevalent in France and responsible for invasive (57.5%) and non-invasive (42.5%) clinical manifestations. We tested phagocytosis and survival in mouse bone marrow-derived macrophages and quantified the pro-inflammatory mediators (IL-6, TNF-α) and type I interferon (INF-β) production. Invasive emm89 isolates were more phagocytosed than their non-invasive counterparts, and emm89 isolates more than the other isolates. Regarding the survival, differences were observed depending on the isolate emm type, but not between invasive and non-invasive isolates within the same emm type. The level of inflammatory mediators produced was also emm type-dependent and mostly invasiveness status independent. Isolates of the emm1 type were able to induce the highest levels of both pro-inflammatory cytokines, whereas emm89 isolates induced the earliest production of IFN-β. Finally, even within emm types, there was a variability of the innate immune responses induced, but survival and inflammatory mediator production were not linked.Márcia DinisCéline PlainvertPavel KovarikMagalie LongoAgnès FouetClaire PoyartPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e101464 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Márcia Dinis Céline Plainvert Pavel Kovarik Magalie Longo Agnès Fouet Claire Poyart The innate immune response elicited by Group A Streptococcus is highly variable among clinical isolates and correlates with the emm type.
description	Group A Streptococcus (GAS) infections remain a significant health care problem due to high morbidity and mortality associated with GAS diseases, along with their increasing worldwide prevalence. Macrophages play a key role in the control and clearance of GAS infections. Moreover, pro-inflammatory cytokines production and GAS persistence and invasion are related. In this study we investigated the correlation between the GAS clinical isolates genotypes, their known clinical history, and their ability to modulate innate immune response. We constituted a collection of 40 independent GAS isolates representative of the emm types currently prevalent in France and responsible for invasive (57.5%) and non-invasive (42.5%) clinical manifestations. We tested phagocytosis and survival in mouse bone marrow-derived macrophages and quantified the pro-inflammatory mediators (IL-6, TNF-α) and type I interferon (INF-β) production. Invasive emm89 isolates were more phagocytosed than their non-invasive counterparts, and emm89 isolates more than the other isolates. Regarding the survival, differences were observed depending on the isolate emm type, but not between invasive and non-invasive isolates within the same emm type. The level of inflammatory mediators produced was also emm type-dependent and mostly invasiveness status independent. Isolates of the emm1 type were able to induce the highest levels of both pro-inflammatory cytokines, whereas emm89 isolates induced the earliest production of IFN-β. Finally, even within emm types, there was a variability of the innate immune responses induced, but survival and inflammatory mediator production were not linked.
format	article
author	Márcia Dinis Céline Plainvert Pavel Kovarik Magalie Longo Agnès Fouet Claire Poyart
author_facet	Márcia Dinis Céline Plainvert Pavel Kovarik Magalie Longo Agnès Fouet Claire Poyart
author_sort	Márcia Dinis
title	The innate immune response elicited by Group A Streptococcus is highly variable among clinical isolates and correlates with the emm type.
title_short	The innate immune response elicited by Group A Streptococcus is highly variable among clinical isolates and correlates with the emm type.
title_full	The innate immune response elicited by Group A Streptococcus is highly variable among clinical isolates and correlates with the emm type.
title_fullStr	The innate immune response elicited by Group A Streptococcus is highly variable among clinical isolates and correlates with the emm type.
title_full_unstemmed	The innate immune response elicited by Group A Streptococcus is highly variable among clinical isolates and correlates with the emm type.
title_sort	innate immune response elicited by group a streptococcus is highly variable among clinical isolates and correlates with the emm type.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/9c5cae4d8391428c8bc358d079e76120
work_keys_str_mv	AT marciadinis theinnateimmuneresponseelicitedbygroupastreptococcusishighlyvariableamongclinicalisolatesandcorrelateswiththeemmtype AT celineplainvert theinnateimmuneresponseelicitedbygroupastreptococcusishighlyvariableamongclinicalisolatesandcorrelateswiththeemmtype AT pavelkovarik theinnateimmuneresponseelicitedbygroupastreptococcusishighlyvariableamongclinicalisolatesandcorrelateswiththeemmtype AT magalielongo theinnateimmuneresponseelicitedbygroupastreptococcusishighlyvariableamongclinicalisolatesandcorrelateswiththeemmtype AT agnesfouet theinnateimmuneresponseelicitedbygroupastreptococcusishighlyvariableamongclinicalisolatesandcorrelateswiththeemmtype AT clairepoyart theinnateimmuneresponseelicitedbygroupastreptococcusishighlyvariableamongclinicalisolatesandcorrelateswiththeemmtype AT marciadinis innateimmuneresponseelicitedbygroupastreptococcusishighlyvariableamongclinicalisolatesandcorrelateswiththeemmtype AT celineplainvert innateimmuneresponseelicitedbygroupastreptococcusishighlyvariableamongclinicalisolatesandcorrelateswiththeemmtype AT pavelkovarik innateimmuneresponseelicitedbygroupastreptococcusishighlyvariableamongclinicalisolatesandcorrelateswiththeemmtype AT magalielongo innateimmuneresponseelicitedbygroupastreptococcusishighlyvariableamongclinicalisolatesandcorrelateswiththeemmtype AT agnesfouet innateimmuneresponseelicitedbygroupastreptococcusishighlyvariableamongclinicalisolatesandcorrelateswiththeemmtype AT clairepoyart innateimmuneresponseelicitedbygroupastreptococcusishighlyvariableamongclinicalisolatesandcorrelateswiththeemmtype
_version_	1718414119289225216

The innate immune response elicited by Group A Streptococcus is highly variable among clinical isolates and correlates with the emm type.

Ejemplares similares