A surfactant polymer dressing potentiates antimicrobial efficacy in biofilm disruption

Abstract A 100% water-soluble surfactant polymer dressing (SPD) that is bio-compatible and non-ionic has been reported to improve wound closure in preliminary clinical studies. The mechanism of action of SPD in wound healing remains unclear. Biofilm infection is a significant problem that hinders pr...

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Autores principales:	Piya Das Ghatak, Shomita S. Mathew-Steiner, Priyanka Pandey, Sashwati Roy, Chandan K. Sen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9c625087692b4692b6da5bd98b288a0a
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spelling	oai:doaj.org-article:9c625087692b4692b6da5bd98b288a0a2021-12-02T15:08:15ZA surfactant polymer dressing potentiates antimicrobial efficacy in biofilm disruption10.1038/s41598-018-19175-72045-2322https://doaj.org/article/9c625087692b4692b6da5bd98b288a0a2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19175-7https://doaj.org/toc/2045-2322Abstract A 100% water-soluble surfactant polymer dressing (SPD) that is bio-compatible and non-ionic has been reported to improve wound closure in preliminary clinical studies. The mechanism of action of SPD in wound healing remains unclear. Biofilm infection is a significant problem that hinders proper wound closure. The objective of this study was to characterize the mechanism of action of SPD inhibition of bacterial biofilm development. Static biofilms (48 h) of the primary wound pathogens Pseudomonas aeruginosa (PA01), Staphylococcus aureus (USA300) were grown on polycarbonate membranes and treated with SPD with and without antibiotics for an additional 24 h. The standard antibiotics – tobramycin (10 μg/ml) for PA01 and rifampicin (10 μg/ml) for USA300, were used in these studies. Following 24 h treatment with and without antibiotics, the biofilms were characterized using scanning electron microscopy (SEM) structural imaging, in vitro imaging system (IVIS) proliferation imaging, colony forming units (CFU), viability assay, quantitative PCR (qPCR) for virulence gene expression. Because SPD is a surfactant based dressing, it potentially has a direct effect on Gram negative bacteria such as Pseudomonas primarily due to the lipid-based outer membrane of the bacteria. SPD is a surfactant based dressing that has potent anti-biofilm properties directly or in synergy with antibiotics.Piya Das GhatakShomita S. Mathew-SteinerPriyanka PandeySashwati RoyChandan K. SenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Piya Das Ghatak Shomita S. Mathew-Steiner Priyanka Pandey Sashwati Roy Chandan K. Sen A surfactant polymer dressing potentiates antimicrobial efficacy in biofilm disruption
description	Abstract A 100% water-soluble surfactant polymer dressing (SPD) that is bio-compatible and non-ionic has been reported to improve wound closure in preliminary clinical studies. The mechanism of action of SPD in wound healing remains unclear. Biofilm infection is a significant problem that hinders proper wound closure. The objective of this study was to characterize the mechanism of action of SPD inhibition of bacterial biofilm development. Static biofilms (48 h) of the primary wound pathogens Pseudomonas aeruginosa (PA01), Staphylococcus aureus (USA300) were grown on polycarbonate membranes and treated with SPD with and without antibiotics for an additional 24 h. The standard antibiotics – tobramycin (10 μg/ml) for PA01 and rifampicin (10 μg/ml) for USA300, were used in these studies. Following 24 h treatment with and without antibiotics, the biofilms were characterized using scanning electron microscopy (SEM) structural imaging, in vitro imaging system (IVIS) proliferation imaging, colony forming units (CFU), viability assay, quantitative PCR (qPCR) for virulence gene expression. Because SPD is a surfactant based dressing, it potentially has a direct effect on Gram negative bacteria such as Pseudomonas primarily due to the lipid-based outer membrane of the bacteria. SPD is a surfactant based dressing that has potent anti-biofilm properties directly or in synergy with antibiotics.
format	article
author	Piya Das Ghatak Shomita S. Mathew-Steiner Priyanka Pandey Sashwati Roy Chandan K. Sen
author_facet	Piya Das Ghatak Shomita S. Mathew-Steiner Priyanka Pandey Sashwati Roy Chandan K. Sen
author_sort	Piya Das Ghatak
title	A surfactant polymer dressing potentiates antimicrobial efficacy in biofilm disruption
title_short	A surfactant polymer dressing potentiates antimicrobial efficacy in biofilm disruption
title_full	A surfactant polymer dressing potentiates antimicrobial efficacy in biofilm disruption
title_fullStr	A surfactant polymer dressing potentiates antimicrobial efficacy in biofilm disruption
title_full_unstemmed	A surfactant polymer dressing potentiates antimicrobial efficacy in biofilm disruption
title_sort	surfactant polymer dressing potentiates antimicrobial efficacy in biofilm disruption
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/9c625087692b4692b6da5bd98b288a0a
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A surfactant polymer dressing potentiates antimicrobial efficacy in biofilm disruption

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