Molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients.

<h4>Background</h4>There is strong evidence that culture-based methods detect only a small proportion of bacteria present in the respiratory tracts of cystic fibrosis (CF) patients.<h4>Methodology/principal findings</h4>Standard microbiological culture and phenotypic identifi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Fadi Bittar, Hervé Richet, Jean-Christophe Dubus, Martine Reynaud-Gaubert, Nathalie Stremler, Jacques Sarles, Didier Raoult, Jean-Marc Rolain
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9c68aec4f6824ea1946d92454a276c7d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9c68aec4f6824ea1946d92454a276c7d
record_format	dspace
spelling	oai:doaj.org-article:9c68aec4f6824ea1946d92454a276c7d2021-11-25T06:11:17ZMolecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients.1932-620310.1371/journal.pone.0002908https://doaj.org/article/9c68aec4f6824ea1946d92454a276c7d2008-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18682840/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>There is strong evidence that culture-based methods detect only a small proportion of bacteria present in the respiratory tracts of cystic fibrosis (CF) patients.<h4>Methodology/principal findings</h4>Standard microbiological culture and phenotypic identification of bacteria in sputa from CF patients have been compared to molecular methods by the use of 16S rDNA amplification, cloning and sequencing. Twenty-five sputa from CF patients were cultured that yield 33 isolates (13 species) known to be pathogens during CF. For molecular cloning, 760 clones were sequenced (7.2+/-3.9 species/sputum), and 53 different bacterial species were identified including 16 species of anaerobes (30%). Discrepancies between culture and molecular data were numerous and demonstrate that accurate identification remains challenging. New or emerging bacteria not or rarely reported in CF patients were detected including Dolosigranulum pigrum, Dialister pneumosintes, and Inquilinus limosus.<h4>Conclusions/significance</h4>Our results demonstrate the complex microbial community in sputa from CF patients, especially anaerobic bacteria that are probably an underestimated cause of CF lung pathology. Metagenomic analysis is urgently needed to better understand those complex communities in CF pulmonary infections.Fadi BittarHervé RichetJean-Christophe DubusMartine Reynaud-GaubertNathalie StremlerJacques SarlesDidier RaoultJean-Marc RolainPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 8, p e2908 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fadi Bittar Hervé Richet Jean-Christophe Dubus Martine Reynaud-Gaubert Nathalie Stremler Jacques Sarles Didier Raoult Jean-Marc Rolain Molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients.
description	<h4>Background</h4>There is strong evidence that culture-based methods detect only a small proportion of bacteria present in the respiratory tracts of cystic fibrosis (CF) patients.<h4>Methodology/principal findings</h4>Standard microbiological culture and phenotypic identification of bacteria in sputa from CF patients have been compared to molecular methods by the use of 16S rDNA amplification, cloning and sequencing. Twenty-five sputa from CF patients were cultured that yield 33 isolates (13 species) known to be pathogens during CF. For molecular cloning, 760 clones were sequenced (7.2+/-3.9 species/sputum), and 53 different bacterial species were identified including 16 species of anaerobes (30%). Discrepancies between culture and molecular data were numerous and demonstrate that accurate identification remains challenging. New or emerging bacteria not or rarely reported in CF patients were detected including Dolosigranulum pigrum, Dialister pneumosintes, and Inquilinus limosus.<h4>Conclusions/significance</h4>Our results demonstrate the complex microbial community in sputa from CF patients, especially anaerobic bacteria that are probably an underestimated cause of CF lung pathology. Metagenomic analysis is urgently needed to better understand those complex communities in CF pulmonary infections.
format	article
author	Fadi Bittar Hervé Richet Jean-Christophe Dubus Martine Reynaud-Gaubert Nathalie Stremler Jacques Sarles Didier Raoult Jean-Marc Rolain
author_facet	Fadi Bittar Hervé Richet Jean-Christophe Dubus Martine Reynaud-Gaubert Nathalie Stremler Jacques Sarles Didier Raoult Jean-Marc Rolain
author_sort	Fadi Bittar
title	Molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients.
title_short	Molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients.
title_full	Molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients.
title_fullStr	Molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients.
title_full_unstemmed	Molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients.
title_sort	molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/9c68aec4f6824ea1946d92454a276c7d
work_keys_str_mv	AT fadibittar moleculardetectionofmultipleemergingpathogensinsputafromcysticfibrosispatients AT herverichet moleculardetectionofmultipleemergingpathogensinsputafromcysticfibrosispatients AT jeanchristophedubus moleculardetectionofmultipleemergingpathogensinsputafromcysticfibrosispatients AT martinereynaudgaubert moleculardetectionofmultipleemergingpathogensinsputafromcysticfibrosispatients AT nathaliestremler moleculardetectionofmultipleemergingpathogensinsputafromcysticfibrosispatients AT jacquessarles moleculardetectionofmultipleemergingpathogensinsputafromcysticfibrosispatients AT didierraoult moleculardetectionofmultipleemergingpathogensinsputafromcysticfibrosispatients AT jeanmarcrolain moleculardetectionofmultipleemergingpathogensinsputafromcysticfibrosispatients
_version_	1718414059630493696

Molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patients.

Ejemplares similares