Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration

Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration

Hui Gao,1–3,* Bei Li,1,2,* Lingzhou Zhao,4 Yan Jin1,21State Key Laboratory of Military Stomatology, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, 2Research and Development Center for Tissue Engineering, The Fourth Military Medical University,...

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Autores principales: Gao H, Li B, Zhao LZ, Jin Y
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
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Medicine (General)
R5-920
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spelling oai:doaj.org-article:9c6943240225442d8768fe8a9462feeb2021-12-02T05:02:16ZInfluence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration1178-2013https://doaj.org/article/9c6943240225442d8768fe8a9462feeb2015-06-01T00:00:00Zhttp://www.dovepress.com/influence-of-nanotopography-on-periodontal-ligament-stem-cell-function-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Hui Gao,1–3,* Bei Li,1,2,* Lingzhou Zhao,4 Yan Jin1,21State Key Laboratory of Military Stomatology, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, 2Research and Development Center for Tissue Engineering, The Fourth Military Medical University, Xi’an, Shaanxi, 3Department of Stomatology, PLA 309th Hospital, Beijing, 4State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, Fourth Military Medical University, Xi’an, Shaanxi, People’s Republic of China*These authors contributed equally to this workAbstract: Periodontal regeneration is an important part of regenerative medicine, with great clinical significance; however, the effects of nanotopography on the functions of periodontal ligament (PDL) stem cells (PDLSCs) and on PDLSC sheet based periodontal regeneration have never been explored. Titania nanotubes (NTs) layered on titanium (Ti) provide a good platform to study this. In the current study, the influence of NTs of different tube size on the functions of PDLSCs was observed. Afterward, an ectopic implantation model using a Ti/cell sheets/hydroxyapatite (HA) complex was applied to study the effect of the NTs on cell sheet based periodontal regeneration. The NTs were able to enhance the initial PDLSC adhesion and spread, as well as collagen secretion. With the Ti/cell sheets/HA complex model, it was demonstrated that the PDLSC sheets were capable of regenerating the PDL tissue, when combined with bone marrow mesenchymal stem cell (BMSC) sheets and HA, without the need for extra soluble chemical cues. Simultaneously, the NTs improved the periodontal regeneration result of the ectopically implanted Ti/cell sheets/HA complex, giving rise to functionally aligned collagen fiber bundles. Specifically, much denser collagen fibers, with abundant blood vessels as well as cementum-like tissue on the Ti surface, which well-resembled the structure of natural PDL, were observed in the NT5 and NT10 sample groups. Our study provides the first evidence that the nanotopographical cues obviously influence the functions of PDLSCs and improve the PDLSC sheet based periodontal regeneration size dependently, which provides new insight to the periodontal regeneration. The Ti/cell sheets/HA complex may constitute a good model to predict the effect of biomaterials on periodontal regeneration.Keywords: titanium implant, titania nanotubes, periodontal ligament stem cells, periodontal regeneration, cell sheetsGao HLi BZhao LZJin YDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 4009-4027 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Gao H
Li B
Zhao LZ
Jin Y
Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration
description Hui Gao,1–3,* Bei Li,1,2,* Lingzhou Zhao,4 Yan Jin1,21State Key Laboratory of Military Stomatology, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, 2Research and Development Center for Tissue Engineering, The Fourth Military Medical University, Xi’an, Shaanxi, 3Department of Stomatology, PLA 309th Hospital, Beijing, 4State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, Fourth Military Medical University, Xi’an, Shaanxi, People’s Republic of China*These authors contributed equally to this workAbstract: Periodontal regeneration is an important part of regenerative medicine, with great clinical significance; however, the effects of nanotopography on the functions of periodontal ligament (PDL) stem cells (PDLSCs) and on PDLSC sheet based periodontal regeneration have never been explored. Titania nanotubes (NTs) layered on titanium (Ti) provide a good platform to study this. In the current study, the influence of NTs of different tube size on the functions of PDLSCs was observed. Afterward, an ectopic implantation model using a Ti/cell sheets/hydroxyapatite (HA) complex was applied to study the effect of the NTs on cell sheet based periodontal regeneration. The NTs were able to enhance the initial PDLSC adhesion and spread, as well as collagen secretion. With the Ti/cell sheets/HA complex model, it was demonstrated that the PDLSC sheets were capable of regenerating the PDL tissue, when combined with bone marrow mesenchymal stem cell (BMSC) sheets and HA, without the need for extra soluble chemical cues. Simultaneously, the NTs improved the periodontal regeneration result of the ectopically implanted Ti/cell sheets/HA complex, giving rise to functionally aligned collagen fiber bundles. Specifically, much denser collagen fibers, with abundant blood vessels as well as cementum-like tissue on the Ti surface, which well-resembled the structure of natural PDL, were observed in the NT5 and NT10 sample groups. Our study provides the first evidence that the nanotopographical cues obviously influence the functions of PDLSCs and improve the PDLSC sheet based periodontal regeneration size dependently, which provides new insight to the periodontal regeneration. The Ti/cell sheets/HA complex may constitute a good model to predict the effect of biomaterials on periodontal regeneration.Keywords: titanium implant, titania nanotubes, periodontal ligament stem cells, periodontal regeneration, cell sheets
format article
author Gao H
Li B
Zhao LZ
Jin Y
author_facet Gao H
Li B
Zhao LZ
Jin Y
author_sort Gao H
title Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration
title_short Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration
title_full Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration
title_fullStr Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration
title_full_unstemmed Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration
title_sort influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/9c6943240225442d8768fe8a9462feeb
work_keys_str_mv AT gaoh influenceofnanotopographyonperiodontalligamentstemcellfunctionsandcellsheetbasednbspperiodontalregeneration
AT lib influenceofnanotopographyonperiodontalligamentstemcellfunctionsandcellsheetbasednbspperiodontalregeneration
AT zhaolz influenceofnanotopographyonperiodontalligamentstemcellfunctionsandcellsheetbasednbspperiodontalregeneration
AT jiny influenceofnanotopographyonperiodontalligamentstemcellfunctionsandcellsheetbasednbspperiodontalregeneration
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