A flow-injection chemiluminescent biomimetic immunoassay method using a molecularly imprinted polymer as a biomimetic antibody for the determination of trichlorfon
In this study, a molecularly imprinted polymer (MIP) was synthesized using trichlorfon as a template molecule. The MIP exhibited a high adsorption capacity toward trichlorfon. Using bovine serum albumin as a carrier, trichlorfon hapten was indirectly labeled with luminol, which competed with free tr...
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Taylor & Francis Group
2018
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oai:doaj.org-article:9c6bd1902a64461784e98ce8198fc6962021-11-26T11:19:46ZA flow-injection chemiluminescent biomimetic immunoassay method using a molecularly imprinted polymer as a biomimetic antibody for the determination of trichlorfon0954-01051465-344310.1080/09540105.2017.1364707https://doaj.org/article/9c6bd1902a64461784e98ce8198fc6962018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1364707https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443In this study, a molecularly imprinted polymer (MIP) was synthesized using trichlorfon as a template molecule. The MIP exhibited a high adsorption capacity toward trichlorfon. Using bovine serum albumin as a carrier, trichlorfon hapten was indirectly labeled with luminol, which competed with free trichlorfon for the combination with a biomimetic antibody of MIP. Based on this direct competitive format, a high throughput flow-injection chemiluminescent biomimetic immunoassay method was developed for fast detection of trace trichlorfon. The influencing factors were investigated and optimized. Under the optimal condition, a lower limit of detection (IC15) of 0.0024 mg/L was obtained using this method. This method was also used to detect the trichlorfon spiked in carrot and cabbage samples, and the results were also verified using the method of gas chromatography.Shoumin WangMingdi JiangZeliang JuXuguang QiaoZhixiang XuTaylor & Francis Grouparticleflow injectionchemiluminescencemolecularly imprinted polymerbiomimetic immunoassayAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 159-170 (2018) |
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DOAJ |
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DOAJ |
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EN |
| topic |
flow injection chemiluminescence molecularly imprinted polymer biomimetic immunoassay Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
flow injection chemiluminescence molecularly imprinted polymer biomimetic immunoassay Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Shoumin Wang Mingdi Jiang Zeliang Ju Xuguang Qiao Zhixiang Xu A flow-injection chemiluminescent biomimetic immunoassay method using a molecularly imprinted polymer as a biomimetic antibody for the determination of trichlorfon |
| description |
In this study, a molecularly imprinted polymer (MIP) was synthesized using trichlorfon as a template molecule. The MIP exhibited a high adsorption capacity toward trichlorfon. Using bovine serum albumin as a carrier, trichlorfon hapten was indirectly labeled with luminol, which competed with free trichlorfon for the combination with a biomimetic antibody of MIP. Based on this direct competitive format, a high throughput flow-injection chemiluminescent biomimetic immunoassay method was developed for fast detection of trace trichlorfon. The influencing factors were investigated and optimized. Under the optimal condition, a lower limit of detection (IC15) of 0.0024 mg/L was obtained using this method. This method was also used to detect the trichlorfon spiked in carrot and cabbage samples, and the results were also verified using the method of gas chromatography. |
| format |
article |
| author |
Shoumin Wang Mingdi Jiang Zeliang Ju Xuguang Qiao Zhixiang Xu |
| author_facet |
Shoumin Wang Mingdi Jiang Zeliang Ju Xuguang Qiao Zhixiang Xu |
| author_sort |
Shoumin Wang |
| title |
A flow-injection chemiluminescent biomimetic immunoassay method using a molecularly imprinted polymer as a biomimetic antibody for the determination of trichlorfon |
| title_short |
A flow-injection chemiluminescent biomimetic immunoassay method using a molecularly imprinted polymer as a biomimetic antibody for the determination of trichlorfon |
| title_full |
A flow-injection chemiluminescent biomimetic immunoassay method using a molecularly imprinted polymer as a biomimetic antibody for the determination of trichlorfon |
| title_fullStr |
A flow-injection chemiluminescent biomimetic immunoassay method using a molecularly imprinted polymer as a biomimetic antibody for the determination of trichlorfon |
| title_full_unstemmed |
A flow-injection chemiluminescent biomimetic immunoassay method using a molecularly imprinted polymer as a biomimetic antibody for the determination of trichlorfon |
| title_sort |
flow-injection chemiluminescent biomimetic immunoassay method using a molecularly imprinted polymer as a biomimetic antibody for the determination of trichlorfon |
| publisher |
Taylor & Francis Group |
| publishDate |
2018 |
| url |
https://doaj.org/article/9c6bd1902a64461784e98ce8198fc696 |
| work_keys_str_mv |
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| _version_ |
1718409550821851136 |