Neodiversification of homeologous CLAVATA1-like receptor kinase genes in soybean leads to distinct developmental outcomes

Abstract The CLAVATA pathway that regulates stem cell numbers of the shoot apical meristem has exclusively been studied in Arabidopsis; as such insight into other species is warranted. In this study, a GmCLV1A mutant (F-S562L) with altered lateral organ development, and two mutants of GmNARK, isolat...

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Autores principales:	Saeid Mirzaei, Jacqueline Batley, Tarik El-Mellouki, Shiming Liu, Khalid Meksem, Brett J. Ferguson, Peter M. Gresshoff
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9c76137ecbc94d8797032730aea18ee5
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spelling	oai:doaj.org-article:9c76137ecbc94d8797032730aea18ee52021-12-02T15:06:11ZNeodiversification of homeologous CLAVATA1-like receptor kinase genes in soybean leads to distinct developmental outcomes10.1038/s41598-017-08252-y2045-2322https://doaj.org/article/9c76137ecbc94d8797032730aea18ee52017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08252-yhttps://doaj.org/toc/2045-2322Abstract The CLAVATA pathway that regulates stem cell numbers of the shoot apical meristem has exclusively been studied in Arabidopsis; as such insight into other species is warranted. In this study, a GmCLV1A mutant (F-S562L) with altered lateral organ development, and two mutants of GmNARK, isolated from a Forrest M2 population (EMS-mutated soybean) were studied. GmCLV1A and GmNARK encode for LRR receptor kinases, and share 92% of protein sequence. While GmNARK is critical for systemic regulation of nodulation (new organ made on the root through symbiosis), we show that GmCLV1A functions locally and has no apparent function in nodulation or root development. However, a recessive, loss-of-function mutation (S562L) in a putative S-glycosylation site of GmCLV1A causes stem nodal identity alterations as well as flower and pod abnormalities (deformed flower and pod). The mutant also exhibits a homeotic phenotype, displaying abnormal leaf development/number, vein-derived leaf emergence, and a thick, faciated stem. The mutant phenotype is also temperature-sensitive. Interestingly, a novel truncated version of GmCLV1A was identified upstream of GmCLV1A that is absent from GmNARK, but is present upstream of the GmNARK orthologues, MtSUNN and PvNARK. Taken together, our findings indicate that GmCLV1A acts on shoot architecture, whereas GmNARK, functions in controlling nodule numbers.Saeid MirzaeiJacqueline BatleyTarik El-MelloukiShiming LiuKhalid MeksemBrett J. FergusonPeter M. GresshoffNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saeid Mirzaei Jacqueline Batley Tarik El-Mellouki Shiming Liu Khalid Meksem Brett J. Ferguson Peter M. Gresshoff Neodiversification of homeologous CLAVATA1-like receptor kinase genes in soybean leads to distinct developmental outcomes
description	Abstract The CLAVATA pathway that regulates stem cell numbers of the shoot apical meristem has exclusively been studied in Arabidopsis; as such insight into other species is warranted. In this study, a GmCLV1A mutant (F-S562L) with altered lateral organ development, and two mutants of GmNARK, isolated from a Forrest M2 population (EMS-mutated soybean) were studied. GmCLV1A and GmNARK encode for LRR receptor kinases, and share 92% of protein sequence. While GmNARK is critical for systemic regulation of nodulation (new organ made on the root through symbiosis), we show that GmCLV1A functions locally and has no apparent function in nodulation or root development. However, a recessive, loss-of-function mutation (S562L) in a putative S-glycosylation site of GmCLV1A causes stem nodal identity alterations as well as flower and pod abnormalities (deformed flower and pod). The mutant also exhibits a homeotic phenotype, displaying abnormal leaf development/number, vein-derived leaf emergence, and a thick, faciated stem. The mutant phenotype is also temperature-sensitive. Interestingly, a novel truncated version of GmCLV1A was identified upstream of GmCLV1A that is absent from GmNARK, but is present upstream of the GmNARK orthologues, MtSUNN and PvNARK. Taken together, our findings indicate that GmCLV1A acts on shoot architecture, whereas GmNARK, functions in controlling nodule numbers.
format	article
author	Saeid Mirzaei Jacqueline Batley Tarik El-Mellouki Shiming Liu Khalid Meksem Brett J. Ferguson Peter M. Gresshoff
author_facet	Saeid Mirzaei Jacqueline Batley Tarik El-Mellouki Shiming Liu Khalid Meksem Brett J. Ferguson Peter M. Gresshoff
author_sort	Saeid Mirzaei
title	Neodiversification of homeologous CLAVATA1-like receptor kinase genes in soybean leads to distinct developmental outcomes
title_short	Neodiversification of homeologous CLAVATA1-like receptor kinase genes in soybean leads to distinct developmental outcomes
title_full	Neodiversification of homeologous CLAVATA1-like receptor kinase genes in soybean leads to distinct developmental outcomes
title_fullStr	Neodiversification of homeologous CLAVATA1-like receptor kinase genes in soybean leads to distinct developmental outcomes
title_full_unstemmed	Neodiversification of homeologous CLAVATA1-like receptor kinase genes in soybean leads to distinct developmental outcomes
title_sort	neodiversification of homeologous clavata1-like receptor kinase genes in soybean leads to distinct developmental outcomes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9c76137ecbc94d8797032730aea18ee5
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Neodiversification of homeologous CLAVATA1-like receptor kinase genes in soybean leads to distinct developmental outcomes

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