SERS-based nanobiosensing for ultrasensitive detection of the p53 tumor suppressor

Fabio Domenici, Anna Rita Bizzarri, Salvatore Cannistraro Biophysics and Nanoscience Centre, Faculty of Science, Universit&agrave; della Tuscia, Viterbo, Italy Background: One of the main challenges in biomedicine is improvement of detection sensitivity to achieve tumor marker recognition at...

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Autores principales:	Domenici F, Bizzarri AR, Cannistraro S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9c783d51e8b945dc82d53d90605ce717
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spelling	oai:doaj.org-article:9c783d51e8b945dc82d53d90605ce7172021-12-02T00:36:58ZSERS-based nanobiosensing for ultrasensitive detection of the p53 tumor suppressor1176-91141178-2013https://doaj.org/article/9c783d51e8b945dc82d53d90605ce7172011-09-01T00:00:00Zhttp://www.dovepress.com/sers-based-nanobiosensing-for-ultrasensitive-detection-of-the-p53-tumo-a8313https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Fabio Domenici, Anna Rita Bizzarri, Salvatore Cannistraro Biophysics and Nanoscience Centre, Faculty of Science, Universit&agrave; della Tuscia, Viterbo, Italy Background: One of the main challenges in biomedicine is improvement of detection sensitivity to achieve tumor marker recognition at a very low concentration when the disease is not significantly advanced. A pivotal role in cancer defense is played by the p53 tumor suppressor, therefore its detection with high sensitivity may contribute considerably to early diagnosis of cancer. In this work, we present a new analytical method based on surface-enhanced Raman spectroscopy which could significantly increase the sensitivity of traditional bioaffinity techniques. p53 molecules were anchored to gold nanoparticles by means of the bifunctional linker 4-aminothiophenol (4-ATP). The characteristic vibrational bands of the p53-4-ATP nanoparticle system were then used to identify the p53 molecules when they were captured by a recognition substrate comprising a monolayer of azurin in molecules possessing significant affinity for this tumor suppressor. The Raman signal enhancement achieved by 4-ATP-mediated crosslinking of p53 to 50 nm gold nanoparticles enabled detect of this protein at a concentration down to 5 &times; 10-13 M. Keywords: surface-enhanced Raman spectroscopy, p53, ultrasensitive detection, atomic force microscopyDomenici FBizzarri ARCannistraro SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2033-2042 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Domenici F Bizzarri AR Cannistraro S SERS-based nanobiosensing for ultrasensitive detection of the p53 tumor suppressor
description	Fabio Domenici, Anna Rita Bizzarri, Salvatore Cannistraro Biophysics and Nanoscience Centre, Faculty of Science, Universit&agrave; della Tuscia, Viterbo, Italy Background: One of the main challenges in biomedicine is improvement of detection sensitivity to achieve tumor marker recognition at a very low concentration when the disease is not significantly advanced. A pivotal role in cancer defense is played by the p53 tumor suppressor, therefore its detection with high sensitivity may contribute considerably to early diagnosis of cancer. In this work, we present a new analytical method based on surface-enhanced Raman spectroscopy which could significantly increase the sensitivity of traditional bioaffinity techniques. p53 molecules were anchored to gold nanoparticles by means of the bifunctional linker 4-aminothiophenol (4-ATP). The characteristic vibrational bands of the p53-4-ATP nanoparticle system were then used to identify the p53 molecules when they were captured by a recognition substrate comprising a monolayer of azurin in molecules possessing significant affinity for this tumor suppressor. The Raman signal enhancement achieved by 4-ATP-mediated crosslinking of p53 to 50 nm gold nanoparticles enabled detect of this protein at a concentration down to 5 &times; 10-13 M. Keywords: surface-enhanced Raman spectroscopy, p53, ultrasensitive detection, atomic force microscopy
format	article
author	Domenici F Bizzarri AR Cannistraro S
author_facet	Domenici F Bizzarri AR Cannistraro S
author_sort	Domenici F
title	SERS-based nanobiosensing for ultrasensitive detection of the p53 tumor suppressor
title_short	SERS-based nanobiosensing for ultrasensitive detection of the p53 tumor suppressor
title_full	SERS-based nanobiosensing for ultrasensitive detection of the p53 tumor suppressor
title_fullStr	SERS-based nanobiosensing for ultrasensitive detection of the p53 tumor suppressor
title_full_unstemmed	SERS-based nanobiosensing for ultrasensitive detection of the p53 tumor suppressor
title_sort	sers-based nanobiosensing for ultrasensitive detection of the p53 tumor suppressor
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/9c783d51e8b945dc82d53d90605ce717
work_keys_str_mv	AT domenicif sersbasednanobiosensingforultrasensitivedetectionofthep53tumorsuppressor AT bizzarriar sersbasednanobiosensingforultrasensitivedetectionofthep53tumorsuppressor AT cannistraros sersbasednanobiosensingforultrasensitivedetectionofthep53tumorsuppressor
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SERS-based nanobiosensing for ultrasensitive detection of the p53 tumor suppressor

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