Transcriptomics and Metabolomics Changes Triggered by Inflorescence Removal in Panax notoginseng (Burk.)

The root of Panax notoginseng (Burk.), in which saponins are the major active components, is a famous traditional Chinese medicine used to stop bleeding and to decrease inflammation and heart disease. Inflorescence removal increases the yield and quality of P. notoginseng, but the underlying molecul...

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Autores principales:	Yu Bai, Haijiao Liu, Jianying Pan, Shiyan Zhang, Yixin Guo, Yisha Xian, Zhirong Sun, Zilong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Panax notoginseng inflorescence removal transcriptomics metabolomics saponins flavonoid Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/9c785599b5b54bf196b7e6425d116ce3
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id	oai:doaj.org-article:9c785599b5b54bf196b7e6425d116ce3
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spelling	oai:doaj.org-article:9c785599b5b54bf196b7e6425d116ce32021-11-12T17:12:39ZTranscriptomics and Metabolomics Changes Triggered by Inflorescence Removal in Panax notoginseng (Burk.)1664-462X10.3389/fpls.2021.761821https://doaj.org/article/9c785599b5b54bf196b7e6425d116ce32021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.761821/fullhttps://doaj.org/toc/1664-462XThe root of Panax notoginseng (Burk.), in which saponins are the major active components, is a famous traditional Chinese medicine used to stop bleeding and to decrease inflammation and heart disease. Inflorescence removal increases the yield and quality of P. notoginseng, but the underlying molecular mechanisms are unknown. Here, the differences between inflorescence-removal treatment and control groups of P. notoginseng were compared using transcriptomics and metabolomics analyses. Illumina sequencing of cDNA libraries prepared from the rhizomes, leaves and roots of the two groups independently identified 6,464, 4,584, and 7,220 differentially expressed genes (DEG), respectively. In total, 345 differentially expressed transcription factors (TFs), including MYB and WRKY family members, were induced by the inflorescence-removal treatment. Additionally, 215 DEGs involved in saponin terpenoid backbone biosynthetic pathways were identified. Most genes involved in the mevalonic acid (MVA) and methylerythritol phosphate (MEP) pathways were activated by inflorescence removal. The co-expression analysis showed that the low expression levels of flavonoid biosynthesis-related genes (e.g., C4H and F3H) decreased the biosynthesis and accumulation of some flavonoids after inflorescence removal. The results not only provide new insights into the fundamental mechanisms underlying the poorly studied inflorescence-removal process in P. notoginseng and other rhizome crops, but they also represent an important resource for future research on gene functions during inflorescence-removal treatments and the reproductive stage.Yu BaiHaijiao LiuJianying PanShiyan ZhangYixin GuoYisha XianZhirong SunZilong ZhangFrontiers Media S.A.articlePanax notoginsenginflorescence removaltranscriptomicsmetabolomicssaponinsflavonoidPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Panax notoginseng inflorescence removal transcriptomics metabolomics saponins flavonoid Plant culture SB1-1110
spellingShingle	Panax notoginseng inflorescence removal transcriptomics metabolomics saponins flavonoid Plant culture SB1-1110 Yu Bai Haijiao Liu Jianying Pan Shiyan Zhang Yixin Guo Yisha Xian Zhirong Sun Zilong Zhang Transcriptomics and Metabolomics Changes Triggered by Inflorescence Removal in Panax notoginseng (Burk.)
description	The root of Panax notoginseng (Burk.), in which saponins are the major active components, is a famous traditional Chinese medicine used to stop bleeding and to decrease inflammation and heart disease. Inflorescence removal increases the yield and quality of P. notoginseng, but the underlying molecular mechanisms are unknown. Here, the differences between inflorescence-removal treatment and control groups of P. notoginseng were compared using transcriptomics and metabolomics analyses. Illumina sequencing of cDNA libraries prepared from the rhizomes, leaves and roots of the two groups independently identified 6,464, 4,584, and 7,220 differentially expressed genes (DEG), respectively. In total, 345 differentially expressed transcription factors (TFs), including MYB and WRKY family members, were induced by the inflorescence-removal treatment. Additionally, 215 DEGs involved in saponin terpenoid backbone biosynthetic pathways were identified. Most genes involved in the mevalonic acid (MVA) and methylerythritol phosphate (MEP) pathways were activated by inflorescence removal. The co-expression analysis showed that the low expression levels of flavonoid biosynthesis-related genes (e.g., C4H and F3H) decreased the biosynthesis and accumulation of some flavonoids after inflorescence removal. The results not only provide new insights into the fundamental mechanisms underlying the poorly studied inflorescence-removal process in P. notoginseng and other rhizome crops, but they also represent an important resource for future research on gene functions during inflorescence-removal treatments and the reproductive stage.
format	article
author	Yu Bai Haijiao Liu Jianying Pan Shiyan Zhang Yixin Guo Yisha Xian Zhirong Sun Zilong Zhang
author_facet	Yu Bai Haijiao Liu Jianying Pan Shiyan Zhang Yixin Guo Yisha Xian Zhirong Sun Zilong Zhang
author_sort	Yu Bai
title	Transcriptomics and Metabolomics Changes Triggered by Inflorescence Removal in Panax notoginseng (Burk.)
title_short	Transcriptomics and Metabolomics Changes Triggered by Inflorescence Removal in Panax notoginseng (Burk.)
title_full	Transcriptomics and Metabolomics Changes Triggered by Inflorescence Removal in Panax notoginseng (Burk.)
title_fullStr	Transcriptomics and Metabolomics Changes Triggered by Inflorescence Removal in Panax notoginseng (Burk.)
title_full_unstemmed	Transcriptomics and Metabolomics Changes Triggered by Inflorescence Removal in Panax notoginseng (Burk.)
title_sort	transcriptomics and metabolomics changes triggered by inflorescence removal in panax notoginseng (burk.)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/9c785599b5b54bf196b7e6425d116ce3
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Transcriptomics and Metabolomics Changes Triggered by Inflorescence Removal in Panax notoginseng (Burk.)

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