Protein kinase CK2 in TGFβ-induced lens epithelial-mesenchymal transition

Cataract is a leading cause of reversible blindness. Secondary cataract is the result of migration, proliferation, and epithelial-mesenchymal transformation (EMT) of lens epithelial cells (LECs), induced by transforming growth factor β (TGFβ). The extracellular signal-regulated kinases 1 and 2 (ERK...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alexander Oscar, Vasil Haykin, Violeta Dimitrova, Iva Petkova, Yani Zdravkov, Stanislava Kostova, Nevyana Veleva, Vanyo Mitev, Chavdar Elenkov, Antonia Isaeva
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	cataract fibrosis tgfβ emt erk1/2 ck2 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/9c7fb317bcc5430b83b0c2693e892292
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9c7fb317bcc5430b83b0c2693e892292
record_format	dspace
spelling	oai:doaj.org-article:9c7fb317bcc5430b83b0c2693e8922922021-11-26T11:19:47ZProtein kinase CK2 in TGFβ-induced lens epithelial-mesenchymal transition1310-28181314-353010.1080/13102818.2021.2003720https://doaj.org/article/9c7fb317bcc5430b83b0c2693e8922922021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13102818.2021.2003720https://doaj.org/toc/1310-2818https://doaj.org/toc/1314-3530Cataract is a leading cause of reversible blindness. Secondary cataract is the result of migration, proliferation, and epithelial-mesenchymal transformation (EMT) of lens epithelial cells (LECs), induced by transforming growth factor β (TGFβ). The extracellular signal-regulated kinases 1 and 2 (ERK 1/2) and casein kinase 2 (CK2) are important for fundamental cellular processes. This study aimed to evaluate the role of CK2 in the EMT of LECs. Primary LECs cultures were obtained from age-related and type 2 diabetes-induced cataracts. Diabetic LECs were capable of spontaneous in vitro differentiation. Treatment of LECs with TGFβ2-stimulated ERK1/2 activity, which correlated with the expressed α-smooth muscle actin (α-SMA). siRNA-mediated attenuation of the endogenous catalytic subunit (α) of CK2-inhibited ERK1/2 activity and decreased α-SMA expression. This study shows evidence that the function of ERK1/2 is regulated by the constitutively active heterotetrameric protein kinase CK2 in this cellular phenotype. This study enriches the knowledge on LECs physiology in normal and in pathological conditions.Alexander OscarVasil HaykinVioleta DimitrovaIva PetkovaYani ZdravkovStanislava KostovaNevyana VelevaVanyo MitevChavdar ElenkovAntonia IsaevaTaylor & Francis Grouparticlecataractfibrosistgfβemterk1/2ck2BiotechnologyTP248.13-248.65ENBiotechnology & Biotechnological Equipment, Vol 35, Iss 1, Pp 1680-1685 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cataract fibrosis tgfβ emt erk1/2 ck2 Biotechnology TP248.13-248.65
spellingShingle	cataract fibrosis tgfβ emt erk1/2 ck2 Biotechnology TP248.13-248.65 Alexander Oscar Vasil Haykin Violeta Dimitrova Iva Petkova Yani Zdravkov Stanislava Kostova Nevyana Veleva Vanyo Mitev Chavdar Elenkov Antonia Isaeva Protein kinase CK2 in TGFβ-induced lens epithelial-mesenchymal transition
description	Cataract is a leading cause of reversible blindness. Secondary cataract is the result of migration, proliferation, and epithelial-mesenchymal transformation (EMT) of lens epithelial cells (LECs), induced by transforming growth factor β (TGFβ). The extracellular signal-regulated kinases 1 and 2 (ERK 1/2) and casein kinase 2 (CK2) are important for fundamental cellular processes. This study aimed to evaluate the role of CK2 in the EMT of LECs. Primary LECs cultures were obtained from age-related and type 2 diabetes-induced cataracts. Diabetic LECs were capable of spontaneous in vitro differentiation. Treatment of LECs with TGFβ2-stimulated ERK1/2 activity, which correlated with the expressed α-smooth muscle actin (α-SMA). siRNA-mediated attenuation of the endogenous catalytic subunit (α) of CK2-inhibited ERK1/2 activity and decreased α-SMA expression. This study shows evidence that the function of ERK1/2 is regulated by the constitutively active heterotetrameric protein kinase CK2 in this cellular phenotype. This study enriches the knowledge on LECs physiology in normal and in pathological conditions.
format	article
author	Alexander Oscar Vasil Haykin Violeta Dimitrova Iva Petkova Yani Zdravkov Stanislava Kostova Nevyana Veleva Vanyo Mitev Chavdar Elenkov Antonia Isaeva
author_facet	Alexander Oscar Vasil Haykin Violeta Dimitrova Iva Petkova Yani Zdravkov Stanislava Kostova Nevyana Veleva Vanyo Mitev Chavdar Elenkov Antonia Isaeva
author_sort	Alexander Oscar
title	Protein kinase CK2 in TGFβ-induced lens epithelial-mesenchymal transition
title_short	Protein kinase CK2 in TGFβ-induced lens epithelial-mesenchymal transition
title_full	Protein kinase CK2 in TGFβ-induced lens epithelial-mesenchymal transition
title_fullStr	Protein kinase CK2 in TGFβ-induced lens epithelial-mesenchymal transition
title_full_unstemmed	Protein kinase CK2 in TGFβ-induced lens epithelial-mesenchymal transition
title_sort	protein kinase ck2 in tgfβ-induced lens epithelial-mesenchymal transition
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/9c7fb317bcc5430b83b0c2693e892292
work_keys_str_mv	AT alexanderoscar proteinkinaseck2intgfbinducedlensepithelialmesenchymaltransition AT vasilhaykin proteinkinaseck2intgfbinducedlensepithelialmesenchymaltransition AT violetadimitrova proteinkinaseck2intgfbinducedlensepithelialmesenchymaltransition AT ivapetkova proteinkinaseck2intgfbinducedlensepithelialmesenchymaltransition AT yanizdravkov proteinkinaseck2intgfbinducedlensepithelialmesenchymaltransition AT stanislavakostova proteinkinaseck2intgfbinducedlensepithelialmesenchymaltransition AT nevyanaveleva proteinkinaseck2intgfbinducedlensepithelialmesenchymaltransition AT vanyomitev proteinkinaseck2intgfbinducedlensepithelialmesenchymaltransition AT chavdarelenkov proteinkinaseck2intgfbinducedlensepithelialmesenchymaltransition AT antoniaisaeva proteinkinaseck2intgfbinducedlensepithelialmesenchymaltransition
_version_	1718409551030517760

Protein kinase CK2 in TGFβ-induced lens epithelial-mesenchymal transition

Ejemplares similares